Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 22,271 results for: emb

Clear keywords
Showing: 5641 - 5660 of 22271 results

  1. pGS1022

    Plasmid
    #59576
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Twin-Strep tag and a URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Publication
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    Twin-Strep tag optimized for PCR amplification followed by URA3 selection marker
    Use
    E. coli cloning vector
    Tags
    Twin-Strep tag
    Promoter
    None
    Available since
    Aug. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    59576-plasmid-map-sequence-id-92983

    Enlarge

  2. pEMS1229

    Plasmid
    #29115
    Purpose
    Expression of the reporter gene under the control of the MiniPromoter in this plasmid has not been tested
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple203 (SLC7A5 Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-NLS
    Available since
    Oct. 3, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    29115-plasmid-map-sequence-id-13530

    Enlarge

  4. CibN

    Plasmid
    #209671
    Purpose
    Expresses CIB1 fused with Split-TurboID-N and pmGFP in mammalian cells
    Depositor
    Sanjeevi Sivasankar
    Publication
    Shafraz et al J Cell Sci. 2023 Oct 1;136(19):jcs261430
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CIB1 (CIB1 Mustard Weed)
    Tags
    Split-TurboID-N and plasma membrane-targeted EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    209671-plasmid-map-sequence-id-415908

    Enlarge

  5. HA-BACE1-mBFP

    Plasmid
    #196697
    Purpose
    Expresses Bace1 with an HA tag at N-terminal for single molecule tracking and other measusers in mammalian cells
    Depositor
    Martino Calamai
    Publication
    Capitini et al iScience. 2023 Apr 11;26(5):106611. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Beta-secretase 1 (BACE1 Human)
    Tags
    HA and mBFP
    Expression
    Mammalian
    Available since
    April 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    196697-plasmid-map-sequence-id-386452

    Enlarge

  6. pCS2+-Pard3-EGFP-PIF6

    Plasmid
    #154914
    Purpose
    Full length zebrafish Pard3 (Gene ID: 403050), tagged with EGFP fluorescent protein and Arabidopsis PIF6 (as in construct Addgene ID 154913).
    Depositor
    Clare Buckley, Jonathan Clarke
    Publication
    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    par-3 family cell polarity regulator alpha, b (pard3ab Zebrafish)
    Use
    Xenopus/avian/zebrafish
    Tags
    1-100 amino acids of Arabidopsis PIF6 and EGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    154914-plasmid-map-sequence-id-308721

    Enlarge

  7. pTagGFP2-N SIN1

    Plasmid
    #124919
    Purpose
    For expression of wild-type SIN1-GFP
    Depositor
    Jin Chen
    Publication
    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MAPKAP1 (MAPKAP1 Human)
    Tags
    TagGFP2
    Expression
    Mammalian
    Mutation
    Insert ORF was sirently mutated to be resistent t…
    Available since
    May 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    124919-plasmid-map-sequence-id-244573

    Enlarge

  8. pEGFP-N1 VAMP2 R125TAG

    Plasmid
    #69876
    Purpose
    express in mammalian cells for unnatural amino acid incorporation into VAMP2-GFP
    Depositor
    Silvio Rizzoli
    Publication
    Vreja et al ACS Nano. 2015 Nov 24;9(11):11034-41. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    VAMP2-GFP (Vamp2 Rat)
    Use
    Synthetic Biology
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Amber stop codon in the linker between VAMP2 and …
    Available since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. RAMA-bio-His

    Plasmid
    #50737
    Purpose
    Expresses enzymatically monobiotinylated full length RAMA ectodomain
    Depositor
    Gavin Wright
    Publication
    Zenonos et al Malar J. 2014 Mar 12;13(1):93. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RAMA
    Tags
    ratCD4d3+4,biotinylation sequence, 6xHis
    Expression
    Mammalian
    Mutation
    all potential N-linked glycosylation sites (N-X-S…
    Promoter
    CMV
    Available since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50737-plasmid-map-sequence-id-76050

    Enlarge

  13. pRN3P-NLS-EGFP-deadBtgh-3NLS

    Plasmid
    #194470
    Purpose
    Encodes for catalytically inactive bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.
    Depositor
    Mathieu Boulard
    Publication
    Formichetti et al Dev Cell 10 Jan 2024
    Insert
    BtGH84
    Use
    For in vitro transcription from t3 promoter after…
    Tags
    SV40 Nuclear Localization Signal, SV40 Nuclear Lo…
    Mutation
    Aspartic Acid 242 (in Dennis et al.) changed to A…
    Available since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    194470-plasmid-map-sequence-id-384877

    Enlarge

  14. pTK165_Mem-mCherry-4.1G-CTD

    Plasmid
    #46362
    Depositor
    Iain Cheeseman
    Publication
    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    4.1G C-terminal domain (EPB41L2 Human)
    Tags
    Mem (N-terminal 20 amino acids of neuromodulin fo…
    Expression
    Mammalian
    Mutation
    C-terminal domain from 886-1005 amino acids
    Promoter
    CMV
    Available since
    Aug. 5, 2013
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    46362-plasmid-map-sequence-id-65994

    Enlarge

  15. hSyn ArcLightCo (Q239)-T2A-nls-mCherry

    Plasmid
    #85844
    Purpose
    Genetically encoded voltage sensor ArcLight- codon optimized
    Depositor
    Vincent Pieribone
    Publication
    Platisa et al ACS Chem Neurosci. 2017 Jan 3. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ArcLightCo-Q239
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Ci-VSP contains R217Q mutation; super ecliptic pH…
    Promoter
    hSyn1
    Available since
    Jan. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    85844-plasmid-map-sequence-id-170992

    Enlarge

  16. pOPS0379

    Plasmid
    #115510
    Purpose
    Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing sfGFP
    Depositor
    Philip Poole
    Publication
    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi
    Insert
    Rlv3841pNifH(pOGG082) sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026
    Expression
    Bacterial
    Mutation
    Domesticated for Golden Gate cloning
    Available since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    115510-plasmid-map-sequence-id-224287

    Enlarge

  19. pcDNA_RGG-GFP-RGG_withTEVsite

    Plasmid
    #124939
    Purpose
    Expresion of RGG_GFP-RGG
    Depositor
    Matthew Good, Daniel Hammer, Benjamin Schuster
    Publication
    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    RGG-GFP-RGG (using LAF-1 RGG domain) (laf-1 Nematode)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    TEV site between RGG and EGFP
    Available since
    June 25, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    124939-plasmid-map-sequence-id-247690

    Enlarge

  20. ORAI1-YFP V107M

    Plasmid
    #114180
    Purpose
    ORAI1 channel with C-terminal YFP, carrying the mutation V107M in the protein 1st transmembrane domain, responsible for a constitutive activity and associated with tubular aggregate myopathy (TAM).
    Depositor
    Nicolas Demaurex
    Publication
    Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ORAI1-YFP V107M (ORAI1 Human)
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    V107M
    Available since
    Nov. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    114180-plasmid-map-sequence-id-218674

    Enlarge

Showing: 5641 - 5660 of 22271 results