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pGC4780

Plasmid

#113408

Purpose

Translation reporter with inducible translation coupling (high native GFP expression)

Depositor

Adam Arkin, Guillaume Cambray

Article

Cambray et al Nat Biotechnol. 2018 Nov;36(10):1005-101

Insert

158_33121123_1

Use

Synthetic Biology

Tags

fused to codon deptimized sfGFP with Flexible lin…

Expression

Bacterial

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pBMN-flag beta-catenin K49R

Plasmid

#44750

Depositor

Eric Fearon

Article

Winer et al J Biol Chem. 2006 Sep 8;281(36):26181-7.

Insert

beta-catenin

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

K49R

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pTH646-2µ-RLuc

Plasmid

#40600

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

Renilla Luciferase

Expression

Yeast

Promoter

ADH1

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pAJ20

Plasmid

#14919

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

Strongyloides stercoralis Ribosomal protein small subunit

Tags

GFP(S65C)

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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XFIVP Smad3

Plasmid

#14826

Depositor

Rik Derynck

Article

Feng et al Genes Dev. 1998 Jul 15. 12(14):2153-63.

Insert

Smad3 (SMAD3 Human)

Tags

VP16 activation domain

Expression

Mammalian

Available Since

May 15, 2007

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-mR

Plasmid

#60990

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound dTomato

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

dTomato

Use

Zebrafish

Tags

membrane tag

Promoter

Bactin2

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only
pBMN-flag beta-catenin K19R

Plasmid

#44749

Depositor

Eric Fearon

Article

Winer et al J Biol Chem. 2006 Sep 8;281(36):26181-7.

Insert

beta-catenin

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

K19R

Available Since

March 13, 2014

Availability

Academic Institutions and Nonprofits only
pAJ09

Plasmid

#14913

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis G protein alpha subunit promoter

Tags

GFP(S65C)

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pMTB-Multibow-hfR

Plasmid

#60998

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. . Encodes nuclear mKate2.

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

mKate2

Use

Zebrafish

Tags

histone 2B tag

Promoter

Bactin2

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
pLsCas13a-gRNA-1

Plasmid

#164865

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-1

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB8382

Plasmid

#126916

Purpose

Plasmid containing integration cassette at XI-3 integration site in Saccharomyces cerevisiae for overexpression of XdCrtI

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtI

Expression

Yeast

Available Since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pMTB-Multibow-mB

Plasmid

#60985

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound EBFP2.

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

EBFP2

Use

Zebrafish

Tags

membrane tag

Promoter

Bactin2

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only
LacD_WT_pET21a

Plasmid

#29785

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase

Tags

His

Expression

Bacterial

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
LacD_L165E_pET21a

Plasmid

#29786

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase

Tags

His

Expression

Bacterial

Mutation

L165E

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
LacD_L275S_pET21a

Plasmid

#29787

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase

Tags

His

Expression

Bacterial

Mutation

L275S

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
pTH736-CEN-staHIS3

Plasmid

#38228

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

HIS3 (HIS3 Budding Yeast, Synthetic)

Tags

HA

Expression

Yeast

Promoter

TDH3 (=GPD)

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pPI461

Plasmid

#113215

Purpose

generic knockin vector for C-terminal tagging with mCherry

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Generic knockin vector for c-terminal tagging wit…

Tags

mCherry

Available Since

Jan. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pPI457

Plasmid

#113200

Purpose

shuttle vector, C-terminal mScarlet tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector, c-termin…

Tags

mScarlet

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pPI418

Plasmid

#113199

Purpose

shuttle vector, N-terminal mScarlet tagging

Depositor

Rob Kay

Article

Paschke et al PLoS One. 2018 May 30;13(5):e0196809. do

Type

Empty backbone

Use

Dictyostelium discoideum shuttle vector, n-termin…

Tags

mScarlet

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAJ01

Plasmid

#14908

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis era-1 3' UTR

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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