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pRK5-HA-Ubiquitin-K6R

Plasmid

#121153

Purpose

Mammalian expression of HA tagged ubiquitin K6R

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Expression

Mammalian

Mutation

K6 mutated to an R

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC25A43

Plasmid

#132030

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A43 (SLC25A43 Human)

Expression

Mammalian

Available Since

Oct. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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plentiCRISPR-v2 Puro EIF2AK4/GCN2_sgRNA

Plasmid

#218528

Purpose

sgRNA targeting human EIF2AK4/GCN2

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

EIF2AK4 (EIF2AK4 Human)

Use

CRISPR and Lentiviral

Available Since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-FLT3-V5/HIS

Plasmid

#201985

Purpose

expression of human FLT3 receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

FLT3 receptor tyrosine kinase (FLT3 Human)

Tags

V5, 6xHis

Expression

Mammalian

Promoter

CMV

Available Since

June 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLVNP3.0-PEmax

Plasmid

#206883

Purpose

Expresses FLAG-tagged PEmax fused to Gag through a linker sequence

Depositor

Jacob Giehm Mikkelsen

Article

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Insert

PEmax

Use

CRISPR and Lentiviral

Tags

FLAG

Promoter

CMV

Available Since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Ubiquitin-K6

Plasmid

#121151

Purpose

Mammalian expression of HA tagged ubiquitin with K6 only, other lysines mutated to arginines

Depositor

Josef Kittler

Article

Birsa et al J Biol Chem. 2014 May 23;289(21):14569-8

Insert

Ubiquitin C (UBC Human)

Expression

Mammalian

Mutation

All Ks apart from K6 mutated to Rs

Available Since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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EF1A_Hygro_P2A_RUNX1_R118G_Barcode

Plasmid

#170970

Purpose

Overexpression of RUNX1 R118G mutant protein.

Depositor

Prashant Mali

Article

Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

Insert

RUNX1 (RUNX1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

R118G

Promoter

Ef1A

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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EF1A_Hygro_P2A_RUNX1_G100V_Barcode

Plasmid

#170955

Purpose

Overexpression of RUNX1 G100V mutant protein.

Depositor

Prashant Mali

Article

Ozturk et al Cell Rep. 2024 Jul 4;43(7):114436. doi:

Insert

RUNX1 (RUNX1 Human)

Use

Lentiviral

Expression

Mammalian

Mutation

G100V

Promoter

Ef1A

Available Since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcw57.1-RquA-FLAG

Plasmid

#229685

Purpose

Doxycycline-inducible expression of RquA-FLAG

Depositor

Jessica Spinelli

Article

Valeros et al Cell. 2025 Jan 10:S0092-8674(24)01420-X.

Insert

RquA-FLAG

Use

Lentiviral

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pDule2 - Mj Acd A9

Plasmid

#197652

Purpose

E. coli machinery vector for the genetic encoding of acridone at TAG codons using the M. jannaschii Acd-A9 synthetase/tRNA pair

Depositor

Ryan Mehl

Article

Sungwienwong et al Org Biomol Chem. 2017 May 3;15(17):3603-

Inserts

Mj Acd A9 synthetase
Methanocaldococcus jannaschii tRNA

Tags

None

Expression

Bacterial

Mutation

Y32A, L65D, F108L, Q109E, D158S, L162T

Promoter

GlnS and lpp

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-R26-FLEX-mStr

Plasmid

#135619

Purpose

Donor vector for genomic targeting of a CAG-FLEX-mStrawberry cassette to the mouse Rosa26 locus

Depositor

Lin He

Article

Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

Insert

CAG-FLEX-mStrawberry flanked by Rosa26 homology arms (Gt(ROSA)26Sor Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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Lenti-pHSE::Cre-mRuby3

Plasmid

#164136

Purpose

Lentiviral construct for building stable cell lines expressing Cre recombinase and mRuby3 in the presence of heat induction

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Insert

HSE promoter-Cre-T2A-mRuby3

Use

CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

Expression

Mammalian

Promoter

HSE synthetic promoter

Available Since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ALK-V5/HIS

Plasmid

#200115

Purpose

Expression of human ALK receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

human ALK receptor tyrosine kinase (ALK Human)

Tags

V5/His

Expression

Mammalian

Promoter

CMV

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX VAMP7 (1-188)

Plasmid

#42315

Depositor

Thierry Galli

Article

Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

Insert

VAMP7 Cytoplasmic domain (1-181) (VAMP7 Human)

Tags

GST

Expression

Bacterial

Mutation

Δ181-220

Promoter

Ptac

Available Since

March 12, 2013

Availability

Academic Institutions and Nonprofits only
pMXs_FLAG-SFXN1

Plasmid

#110634

Purpose

Retroviral expression of Flag-SFXN1 in mammalian cells

Depositor

David Sabatini

Article

Kory et al Science. 2018 Nov 16;362(6416). pii: 362

Insert

SFXN1 (SFXN1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

codon-optimized cDNA (no amino acid mutations)

Available Since

Nov. 19, 2018

Availability

Academic Institutions and Nonprofits only

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PGL4-4X Mut Renilla

Plasmid

#70040

Purpose

Control plasmid for testing 5'UTR folding elements

Depositor

Hans-Guido Wendel

Article

Wolfe et al Nature. 2014 Sep 4;513(7516):65-70. doi:

Insert

Random sequence matched for GC-content

Use

Luciferase

Tags

renilla luciferase

Expression

Mammalian

Promoter

SV40

Available Since

Dec. 17, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP VAMP7 (1-120)

Plasmid

#42317

Depositor

Thierry Galli

Article

Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

Insert

VAMP7 Longin domain (1-120) (VAMP7 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Δ120-220

Promoter

CMV

Available Since

March 1, 2013

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC22A8

Plasmid

#131989

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC22A8 (SLC22A8 Human)

Expression

Mammalian

Available Since

Oct. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-mTagBFP2-FRB-Rab7

Plasmid

#214273

Purpose

Mammalian expression of mTagBFP2-FRB-Rab7 (late endosome marker)

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

mTagBFP2-FRB-Rab7A (RAB7A Canis lupus familiaris (Dog))

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pPN089

Plasmid

#91614

Purpose

Express sgRNA targeting human FURIN

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

FURIN (FURIN Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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pLV.tetO.lmx1a

Plasmid

#234847

Purpose

3rd generation lentiviral vector, expresses Lmx1a under control of TetON promoter

Depositor

Malin Parmar

Article

Nolbrant et al Stem Cell Reports. 2020 Oct 13;15(4):869

Insert

Lmx1a (Lmx1a Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

May 15, 2025

Availability

Academic Institutions and Nonprofits only

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INS-CRa-Lsg-MS2

Plasmid

#247478

Purpose

sgRNA for INS activation cloned into LsgRNA-MS2 backbone

Depositor

Ting Zhou

Article

Wu et al Stem Cell Reports. 2025 Apr 25:102499. d

Insert

sgRNA targeting INS promoter (INS Human, Synthetic)

Use

CRISPR

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pLVX-Flag-MIB1-IRES-Puro

Plasmid

#140240

Purpose

Lentiviral vector expressing flag-tagged MIB1

Depositor

Urs Greber

Article

Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

Insert

Flag-MIB1 (MIB1 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 15, 2020

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-NfiA

Plasmid

#64901

Purpose

Inducible expression of murine CDS encoding for NfiA. Toghther with TetO-FUW-Sox9 and TetO-FUW-NfiB allows reprograming into iAstro. cells.

Depositor

Vania Broccoli

Article

Caiazzo et al Stem Cell Reports. 2015 Jan 13;4(1):25-3

Insert

Nfia (Nfia Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

TetO

Available Since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pT-Rex Dest30 dn-cHSF1

Plasmid

#134736

Purpose

Mammalian expression vector for inducible expression of a constitutively active dominant-negative HSF1

Depositor

Matthew D. Shoulders

Article

Moore et al ACS Chem Biol. 2016 Jan 15;11(1):200-10.

Insert

dn-cHSF1 (HSF1 Human)

Expression

Mammalian

Mutation

Deletion of amino acids 186-202, 379−529

Promoter

CMV/TO

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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COX8mls-Flag-APEX2-SOPP3

Plasmid

#229075

Purpose

Expresses APEX2-SOPP3 in the mitochondrial matrix

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express APEX2-SOPP3 in the mitochondrial matrix (APEX2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC25A45

Plasmid

#132054

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A45 (SLC25A45 Human)

Expression

Mammalian

Available Since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC6A19

Plasmid

#132231

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC6A19 (SLC6A19 Human)

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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hGFAP-fLuc

Plasmid

#40589

Depositor

Albee Messing

Article

Cho et al J Neurochem. 2009 Jul;110(1):343-51. Epu

Insert

hGFAP promoter (GFAP Human)

Use

Luciferase

Tags

Firefly luciferase

Mutation

Contains human GFAP promoter fragment (-2163 to +…

Promoter

GFAP promoter

Available Since

Sept. 20, 2012

Availability

Academic Institutions and Nonprofits only

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mCherry-PH(VPS13C)

Plasmid

#232869

Purpose

Expresses the PH domain of VPS13C in mammalian cells

Depositor

Pietro De Camilli

Article

Wang et al Nat Cell Biol. 2025 May;27(5):776-789. d

Insert

the PH domain of VPS13C (VPS13C Human)

Tags

mCherry

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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