We narrowed to 7,813 results for: ALP

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pmCherry-NT-Tagging

Plasmid

#165870

Purpose

Template for amplification of mCherry N-terminal tagging cassette

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Vector - NT Tagging

Use

Synthetic Biology

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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CMVTCRa_candidate8

Plasmid

#165005

Purpose

Expression of candidate TCR #8 TCRalpha cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRAV (TRA Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCL21-decoder

Plasmid

#159448

Purpose

Insert: Pact1-lexA-ER-B112-phosphodegron-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is degraded upon addition of alpha factor. Backbone: pRG201 (addGene). Marker: Met15.

Depositor

Joerg Stelling

Article

Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

Insert

Pact1-lexA-ER-B112-phosphodegron-tCyc1

Use

Synthetic Biology

Expression

Yeast

Promoter

act1

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCL33-decoder

Plasmid

#159451

Purpose

Insert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.

Depositor

Joerg Stelling

Article

Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

Insert

Pfus1mut-tetR-nls-malE-tCyc1

Use

Synthetic Biology

Expression

Yeast

Promoter

Pfus1mut

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCL38-decoder

Plasmid

#159452

Purpose

Insert: Pfus1tet-lexA-ER-B112-tCyc1.The transcription factor LexA-ER-B112 (see addgene FRP880) is inducible by alpha factor, and repressible by TetR. Backbone: pRG235 (addgene). Marker: Leu2

Depositor

Joerg Stelling

Article

Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:

Insert

Pfus1tet-LexA-ER-B112-tCyc1

Use

Synthetic Biology

Expression

Yeast

Promoter

Pfus1tet

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4xh7SKPylT_EF1_SMIM38*_Intein_DD

Plasmid

#162814

Purpose

STELLA plasmid for amber suppression of C-terminally labelled SMIM38

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

SMIM38(C-TAG)-Intein-DD

Use

Piggybac

Expression

Mammalian

Mutation

SMIM38(Ochre52,Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub(G75,76A)-BsmBI

Plasmid

#162803

Purpose

Control STELLA destination plasmid for N-terminally labelled proteins

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub(G75,76A)-BsmBI

Use

Piggybac

Expression

Mammalian

Mutation

Ubiquitin (G75,76A)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS1_4x7SKPylT_EF1_Ub-*SMIM38

Plasmid

#162808

Purpose

STELLA plasmid for amber suppression of N-terminally labelled SMIM38

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)SMIM38

Use

Piggybac

Expression

Mammalian

Mutation

SMIM38 (Met1, Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pAS_4x7SKPylT_EF1_Ub-*PIGBOS

Plasmid

#162809

Purpose

STELLA plasmid for amber suppression of N-terminally labelled PIGBOS

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

Ub-(N-TAG)PIGBOS

Use

Piggybac

Expression

Mammalian

Mutation

PIGBOS (Met1, Amber)

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1 Puro shRNA PIK3C2A KD5

Plasmid

#162006

Purpose

PIK3C2alpha shRNA

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

PIK3C2A (PIK3C2A Canis Lupus)

Use

Lentiviral

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pJUMP18-lacZ_O

Plasmid

#127027

Purpose

Basic Part O- ORF; lacZ alpha-peptide; blue/white screening.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part lacZ_o

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pTD3685

Plasmid

#113391

Purpose

alpha factor inducible Ty3

Depositor

Suzanne Sandmeyer

Article

Bilanchone et al PLoS Genet. 2015 Sep 30;11(9):e1005528.

Insert

Ty3

Expression

Yeast

Available Since

Oct. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pUPD2-ATPAMTS (12)

Plasmid

#101696

Purpose

ATPAMTS in domestication vector

Depositor

Elena Caro Bernat

Article

Perez-Gonzalez et al BMC Biotechnol. 2017 Nov 13;17(1):80. do

Insert

CCAT-[MTS from S. cerevisiae ATP synthase subunit alpha (YBL099W)]-AATG

Expression

Yeast

Available Since

July 24, 2018

Availability

Academic Institutions and Nonprofits only

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pTU1-C-lacZ

Plasmid

#72937

Purpose

Level 1 Destination vector - Position C

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 1 - C site

Use

Synthetic Biology

Mutation

Beta-lactamase - G717C (nucleotide) - silent muta…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTU1-D-lacZ

Plasmid

#72938

Purpose

Level 1 Destination vector - Position D

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Insert

Level 1 - D site

Use

Synthetic Biology

Mutation

Beta-lactamase - G717C (nucleotide) - silent muta…

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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mOrange2-Cx43-7

Plasmid

#57951

Purpose

Localization: Gap Junctions, Excitation: 549, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Cx43

Tags

mOrange2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
mKO2-Cx43-7

Plasmid

#57867

Purpose

Localization: Gap Junctions, Excitation: 551, Emission: 565

Depositor

Michael Davidson

Article

Shaner et al Nat Methods. 2008 Jun;5(6):545-51. doi:

Insert

Cx43

Tags

mKO2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only
mCerulean3-Cx43-7

Plasmid

#55411

Purpose

Localization: Gap Junctions, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

Cx43

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
TAL3385

Plasmid

#42848

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-tnfb-right (tnfb Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
TAL3384

Plasmid

#42847

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-tnfb-left (tnfb Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2013

Availability

Academic Institutions and Nonprofits only