We narrowed to 7,128 results for: SIM
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Plasmid#82335PurposeVector targeting the mtagBFP2 gene to the GAPDH locus of human cells. BFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.DepositorInsertmtagBFP2-IRESMuro
UseSynthetic BiologyExpressionMammalianAvailable SinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-EGFR*-CUbo
Plasmid#212821PurposeConstitutive or doxycycline-inducible expression of EGFR*-CUbo in mammalian cellsDepositorAvailable SinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp3d_Ub2_PLPro
Plasmid#156472PurposeBacterial expression of Sars-CoV2 Nsp3d_Ub2_PLPro protein with His-tag and GST-tagDepositorInsertNsp3d_Ub2_PLPro (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-Halo-hMRE11(DB1Δ/DB2Δ)
Plasmid#208395PurposeTo generate stable cell line expressing Halo-human MRE11(DB1Δ/DB2Δ) by Retroviral infection.DepositorInsertHuman MRE11 (MRE11 Human)
UseLuciferase and RetroviralExpressionMammalianMutationDB1delta/DB2deltaAvailable SinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-VQR-P2A-GFP-PGK-Puro
Plasmid#110861PurposeLentiviral vector for constitutive expression of Cas9-VQR-P2A-GFP (not codon optimized)DepositorInsertCas9-VQR
UseLentiviralTags3X FLAGMutationD1135V, R1335Q, T1337RPromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR-HOXD13(+7A)-IDR-mCherry-CRY2-NLS
Plasmid#145261PurposeMammalian Expression of Nuclear Opto HOXD13(+7A) IDR with SV40 NLSDepositorAvailable SinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_Nsp1
Plasmid#156465PurposeBacterial expression of Sars-CoV2 Nsp1 protein with His-tag and GST-tagDepositorInsertNsp1 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pROS11
Plasmid#107925PurposeamdS based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacerDepositorInsertgRNA-CAN1.Y, gRNA-ADE2.Y
UseCRISPRExpressionYeastAvailable SinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-mCherry-IRESMygro
Plasmid#82505PurposeVector targeting the mCherry gene to the GAPDH locus of human cells. mCherry is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.DepositorInsertmCherry-IRESMygro
UseSynthetic BiologyExpressionMammalianAvailable SinceSept. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
R979-X58-008: Hs.KRAS4b(1-188)
Plasmid#159691PurposeBaculovirus protein expression of KRAS4b (1-188)DepositorInsertHs.KRAS4b(1-188) (KRAS Human)
ExpressionInsectAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_N_CTD
Plasmid#156484PurposeBacterial expression of Sars-CoV2 N_CTD protein with His-tag and GST-tagDepositorInsertN_CTD (N Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pETM33_N_NTD
Plasmid#156483PurposeBacterial expression of Sars-CoV2 N_NTD protein with His-tag and GST-tagDepositorInsertN_NTD (N Severe acute respiratory syndrome coronavirus 2, Synthetic)
TagsHis-GSTExpressionBacterialMutationGene insert is codon optimized for Ecoli.PromoterT7/LacOAvailable SinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR-GAL4-IL35-IRES-mCherry-PGK-BFP
Plasmid#223210PurposeLentiviral vector - mCherry reporter and IL35 for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorAvailable SinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-GAL4-PDL1-IRES-mCherry-PGK-BFP
Plasmid#223208PurposeLentiviral vector - mCherry reporter and PDL1 for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorAvailable SinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-xBE3-P2A-Puro
Plasmid#110870PurposeLentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cellsDepositorInsertxCas9(3.7)-BE3
UseLentiviralMutationD10A, A262T, R324L, S409I, E480K, E543D, M694I, E…PromoterEF1sAvailable SinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCVhyg-HA-FLAG_Ubc9_WT
Plasmid#164936PurposeExpresses FLAG & HA tagged human uman Ubiquitin Conjugating Enzyme 9 (UBC9) wild type cDNADepositorAvailable SinceMay 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGAPTrap-eGFP-IRESMygro
Plasmid#82503PurposeVector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH. Hygromycin B resistance is encoded by the Mygro gene.DepositorInserteGFP-IRESMygro
UseSynthetic BiologyExpressionMammalianAvailable SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pROF346
Plasmid#155331PurposeBinary vector for expression of PULSE system (under the control of AtUbi10 promoter) controlling Venus-H2B. It constitutively expresses Cerulean-NLS and a plant selection cassette.DepositorInsertRB_KanR_Tnos-NLS-PIF6-E-PUbi10_Tnos-nCerulean-PUbi10_Tnos-NLS-VP16-PhyB-PUbi10_Tnos-EL222-NLS-SRDX-PUbi10_T35S-nVenus-POpto_LB
UseSynthetic BiologyExpressionPlantAvailable SinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Triple ORF LoxP ifgMosaic Donor (LH208)
Plasmid#99625PurposeTo clone the 3 ORFs for Cre dependent generation of ifgMosaicsDepositorInsertN-PhiM
UseCre/Lox, Mouse Targeting, and Synthetic BiologyAvailable SinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hAR-CA576-Z
Plasmid#89108Purposemammalian expression of full-length human androgen receptor with DNA binding domain mutation CA576DepositorAvailable SinceApril 21, 2017AvailabilityAcademic Institutions and Nonprofits only