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pC247

Plasmid

#65890

Purpose

Strong P2 activator plasmid for two-strain system

Depositor

Matthew Bennett

Article

Chen et al Science. 2015 Aug 28;349(6251):986-9. do

Insert

RhlI (rhlI )

Tags

LAA tagged

Expression

Bacterial

Promoter

Prhl/lac-s

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pAW209

Plasmid

#113188

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLKO-shcIAP1A

Plasmid

#44129

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

cIAP1

Use

Lentiviral and RNAi

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
Ypet-RBD

Plasmid

#105291

Purpose

To measure Rho activity by binding and FRET analysis

Depositor

Kenneth Yamada

Article

Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

Insert

RBD (Rtkn Mouse)

Tags

Ypet

Expression

Mammalian

Mutation

aa encoding 7-89

Available Since

Feb. 21, 2018

Availability

Academic Institutions and Nonprofits only

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KaiB-FLAG

Plasmid

#41885

Depositor

Michael Rust

Article

Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

Insert

Kai B

Tags

FLAG

Expression

Bacterial

Promoter

t7 promoter

Available Since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
NOR gate-V2.0

Plasmid

#44452

Depositor

Drew Endy

Article

Bonnet et al Science. 2013 May 3;340(6132):599-603. d

Insert

NOR-gate

Use

Synthetic Biology

Expression

Bacterial

Available Since

May 13, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-TadA-TadA*

Plasmid

#125661

Purpose

Expresses the TadA-TadA* dimer in mammalian cells

Depositor

David Liu

Article

Rees et al Sci Adv. 2019 May 8;5(5):eaax5717. doi:

Insert

TadA-TadA*

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) TRIM12A

Plasmid

#87061

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM12A (Trim12a Mouse)

Expression

Mammalian

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) TRIM12C

Plasmid

#87080

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM12C (Trim12c Mouse)

Expression

Mammalian

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pNR160

Plasmid

#79542

Purpose

Output plasmid: contains 5-state register made up of TP901 and BxbI recognition sites.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Type

Empty backbone

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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pDestTol2pA2-LA-nanoLuc-RA

Plasmid

#128692

Purpose

Donor plasmid that promotes integration of the NanoLuc coding sequence at the apobb.1 locus

Depositor

Steven Farber

Article

Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi:

Insert

apobb.1 NanoLuc

Use

Multisite-gateway assembly

Tags

NanoLuc

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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KvAvs3

Plasmid

#188792

Purpose

Avs3 from Klebsiella variicola PF-1

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

KvAvs3

Expression

Bacterial

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pBABE-FLAG-hG9a

Plasmid

#33023

Depositor

Martin Walsh

Article

Nishio et al Proc Natl Acad Sci U S A. 2004 Aug 3;101

Insert

hG9a

Use

Retroviral

Tags

Flag

Expression

Mammalian

Promoter

5'LTR

Available Since

April 2, 2012

Availability

Academic Institutions and Nonprofits only
pJW1504

Plasmid

#62358

Purpose

RPS2 HA-TEV-AVI pop-in/pop-out plasmid

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

RPS2 fragment

Use

Yeast targeting

Tags

HA-TEV-AVI

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pFF746

Plasmid

#61451

Purpose

aTc-inducible SCRIBE_galK(ON)

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

SCRIBE_galK(ON)

Use

Synthetic Biology

Promoter

pTetO

Available Since

March 6, 2015

Availability

Academic Institutions and Nonprofits only

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NaAtm1

Plasmid

#78308

Purpose

Atm1 type ABC Exporter

Depositor

Douglas Rees

Article

Lee et al Science. 2014 Mar 7;343(6175):1133-6. do

Insert

Atm1 type ABC Exporter

Tags

6x Histidine Tag

Promoter

T7 Promoter

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only
PB-PGK-FKBP-Ztetv-Cit-tvhc-DHFR

Plasmid

#116052

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

FKBP-Ztetv-Cit-tvhc-DHFR

Expression

Mammalian

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pDA12

Plasmid

#113663

Purpose

Cloning vector for bacterial expression with Pdhfr promoter

Depositor

Miguel Valvano

Article

Aubert et al Infect Immun. 2008 May;76(5):1979-91. do

Type

Empty backbone

Expression

Bacterial

Promoter

Pdhfr

Available Since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pJW1514

Plasmid

#62384

Purpose

pCR2.1 linker-tev-linker-3X-FLAG::KanMX

Depositor

Jonathan Weissman

Article

Williams et al Science. 2014 Nov 7;346(6210):748-51. do

Insert

tev 3x FLAG

Use

Yeast c-terminal tagging vector

Tags

linker-tev-linker-3xflag

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pNR284

Plasmid

#79550

Purpose

Output plasmid: contains 16-state register that regulates expression of GFP.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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