Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 31,602 results for: REP

Clear keywords
Showing: 5701 - 5720 of 31602 results

  1. pIFU001

    Plasmid
    #174298
    Purpose
    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) with the E484K mutation and can be combined with pJS697 (Addgene plasmid # 168778) through homologous recombination
    Depositor
    Timothy Whitehead
    Article
    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:
    Insert
    Spike RBD N343Q, E484K (S Severe acute respiratory syndrome coronavirus 2)
    Tags
    HA and cMyc
    Expression
    Yeast
    Mutation
    N343Q, E484K
    Available Since
    Sept. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    174298-plasmid-map-sequence-id-342193

    Enlarge

  3. Donor-CD55-NeoR

    Plasmid
    #153551
    Purpose
    Used as a donor vector for the targeted knock-in of a CD55 mutation. This plasmid carries a neomycin resistance gene cassette
    Depositor
    Hiroyuki Konishi
    Article
    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7
    Insert
    mutant CD55 (a 1415-bp fragment from intron 3, exon 4 and intron 4, with a 1-bp mutation in exon 4), divided into 5' and 3' arms
    Use
    AAV and Cre/Lox; Donor plasmid/targeting vector
    Tags
    N/A
    Mutation
    a 1-bp mutation in exon 4
    Promoter
    No promoter
    Available Since
    March 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    153551-plasmid-map-sequence-id-326384

    Enlarge

  4. Donor-PIGA-NeoR

    Plasmid
    #153549
    Purpose
    Used as a donor vector for the targeted knock-in of a PIGA mutation. This plasmid carries a neomycin resistance gene cassette
    Depositor
    Hiroyuki Konishi
    Article
    Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7
    Insert
    mutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6), divided into 5' and 3' arms
    Use
    AAV and Cre/Lox; Donor plasmid/targeting vector
    Tags
    N/A
    Mutation
    a 3-bp mutation in exon 6
    Promoter
    No promoter
    Available Since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    153549-plasmid-map-sequence-id-319273

    Enlarge

  5. pCC_11 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9-NLS-2A-Puro-WPRE

    Plasmid
    #139096
    Purpose
    Expresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Article
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    KRAB-dxCas9 3.7
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…
    Available Since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139096-plasmid-map-sequence-id-273894

    Enlarge

  9. pCDNA3_shRNA resistant nmPKA-Cα-mEGFP

    Plasmid
    #114144
    Purpose
    The G2A mutant of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.
    Depositor
    Haining Zhong
    Article
    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi
    Insert
    mouse PKA Cα-mEGFP with G2A mutation and silent mutation to resist shRNA knockdown (Prkaca Mouse)
    Expression
    Mammalian
    Mutation
    G2A mutation and silent mutation to resist shRNA …
    Available Since
    Oct. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    114144-plasmid-map-sequence-id-224274

    Enlarge

  12. AAV2_hSyn_Aurora_CA_Citrine

    Plasmid
    #98219
    Purpose
    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with green light, inactivation max with 625 nm (accelarates closure to ms). Not recommended for in vivo use. Codon optimized for mammalian expression.
    Depositor
    Simon Wiegert
    Article
    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.
    Insert
    Synthetic construct Aurora_C128A gene
    Use
    AAV
    Tags
    Citrine
    Expression
    Mammalian
    Mutation
    V59S, E83N, E90Q, E101S, V117R, E123S, C128A, P24…
    Promoter
    human synapsin
    Available Since
    Aug. 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    98219-plasmid-map-sequence-id-256052

    Enlarge

  17. Clover2-N1

    Plasmid
    #54537
    Purpose
    Localization: N1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 H231L based on Bajar et. al. Sci Rep. 2016.
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    Clover2
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54537-plasmid-map-sequence-id-127234

    Enlarge

  18. Clover2-C1

    Plasmid
    #54711
    Purpose
    Localization: C1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 H231L based on Bajar et. al. Sci Rep. 2016.
    Depositor
    Michael Davidson
    Type
    Empty backbone
    Tags
    Clover2
    Expression
    Mammalian
    Available Since
    June 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. KBB258

    Plasmid
    #185072
    Purpose
    Bacterial expression of N-terminus and FXFG repeats of NUP1 nucleoporin as GST-Nup1-N+Rep fusion
    Depositor
    Ken Belanger
    Insert
    NUP1
    Tags
    GST
    Expression
    Yeast
    Mutation
    Nup1 N-term+FXFG repeats
    Available Since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    185072-plasmid-map-sequence-id-368132

    Enlarge

  20. pAAV2/5

    Plasmid
    #232922
    Purpose
    AAV packaging plasmid expressing AAV2 Rep protein and AAV5 capsid protein
    Depositor
    Melina Fan, Addgene Research Program
    Insert
    Rep2/Cap5
    Use
    AAV
    Expression
    Mammalian
    Available Since
    Sept. 17, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    232922-plasmid-map-sequence-id-458562

    Enlarge

Showing: 5701 - 5720 of 31602 results