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pcDNA5/FRT/TO_Lyn11-HaloTag7

Plasmid

#175533

Purpose

Expression of HaloTag7 at the inner leaflet of the plasma membrane of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Lyn11-HaloTag7

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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mRuby2-pBAD

Plasmid

#54771

Purpose

Localization: Protein Expression Vector , Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Type

Empty backbone

Tags

6xHis and mRuby2

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-CEP41_Halo

Plasmid

#135446

Purpose

Mamalian cell expression of HaloTag7 localized at the centrosome

Depositor

Kai Johnsson

Article

Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

Insert

CEP41-HaloTag7

Tags

CEP41

Expression

Mammalian

Promoter

CMV/TetO

Available Since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_b4g-HaloTag9

Plasmid

#175546

Purpose

Expression of HaloTag9 at the Golgi apparatus of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Beta4Gal-HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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mTFP1-C1

Plasmid

#54613

Purpose

Localization: C1 Cloning Vector, Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Type

Empty backbone

Tags

mTFP1

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

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mKusabira Orange-C1 (mKO)

Plasmid

#54834

Purpose

Localization: C1 Cloning Vector, Excitation: 548, Emission: 561

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

Type

Empty backbone

Tags

mKusabira Orange

Expression

Mammalian

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pEJS2699-CMV-Nme2Cas9-E932R

Plasmid

#228720

Purpose

Expresses Nme2 Cas9 nuclease with E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Nme2Cas9-E932R nuclease

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2696-CMV-Nme2SmuCas9-D56R

Plasmid

#228717

Purpose

Expresses Nme2Smu Cas9 nuclease with D56R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Nme2SmuCas9-D56R nuclease

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, D56R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2697-CMV-Nme2SmuCas9-E932R

Plasmid

#228718

Purpose

Expresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Nme2SmuCas9-E932R nuclease

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2698-CMV-Nme2Cas9-D56R

Plasmid

#228719

Purpose

Expresses Nme2 Cas9 nuclease with D56R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv November 07, 2024

Insert

Nme2Cas9-D56R nuclease

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9 D56R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-SiT-N-15

Plasmid

#55325

Purpose

Localization: Golgi TGN, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

SiT (ST6GAL1 Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

aa 1-45 of SiT

Promoter

CMV

Available Since

Oct. 24, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA3-HA-Uch37

Plasmid

#19415

Depositor

Joan Conaway, Ronald Conaway

Article

Yao et al Nat Cell Biol. 2006 Sep . 8(9):994-1002.

Insert

Uch37 (UCHL5 Human)

Tags

2XHA

Expression

Mammalian

Available Since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Beta4Gal-HaloTag7

Plasmid

#175536

Purpose

Expression of HaloTag7 at the at the Golgi apparatus of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

Beta4Gal-HaloTag7

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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MiCy-pBAD

Plasmid

#54565

Purpose

Localization: Protein Expression Vector , Excitation: 472, Emission: 495

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Karasawa et al Biochem J. 2004 Jul 1. 381(Pt 1):307-12.

Type

Empty backbone

Tags

6xHis and MiCy

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only
pNTI819

Plasmid

#216628

Purpose

Cal-ID (V5) for mammalian expression and lentivirus production

Depositor

Nicholas Ingolia

Article

Kim et al Nat Chem Biol. 2024 Jul;20(7):894-905. d

Insert

Cal-ID

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_HaloTag9_SKL

Plasmid

#175542

Purpose

Peroxysomal expression of HaloTag9 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag9-SKL

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pDD284

Plasmid

#66825

Purpose

TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Article

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

TagRFP-T-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized TagRFP-T

Expression

Worm

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only
mEmerald-Cx43-7

Plasmid

#54055

Purpose

Localization: Gap Junctions, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Fong et al Autophagy. 2012 May 1;8(5):794-811. doi:

Insert

Cx43

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pcDNA5/FRT/TO_IgKchL_HA_HaloTag7_myc_PDGFRtmb

Plasmid

#175534

Purpose

Expression of HaloTag7 at the outer leaflet of the plasma membrane of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

IgKchL_HA-HaloTag7-myc_PDGFRtmb

Expression

Mammalian

Available Since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-MyD88-FW/AA

Plasmid

#12289

Depositor

Bruce Beutler

Article

Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

MyD88 (MYD88 Human)

Tags

HA

Expression

Mammalian

Mutation

F285A/W286A

Available Since

Jan. 25, 2007

Availability

Academic Institutions and Nonprofits only