We narrowed to 24,999 results for: Nes

1. pDONR223-BMP2KL

   Plasmid

   #23399

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   BMP2KL (BMP2KL Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

2. pDONR223-NME5

   Plasmid

   #23772

   Depositor

   William Hahn, David Root

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   NME5 (NME5 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pCDF_AtRaf1/Raf2/RbcX2/BSD2/RbcX1

   Plasmid

   #229510

   Purpose

   Expresses Arabidopsis thaliana chaperones: Raf1,Raf2,RbcX2,BSD2,RbcX1

   Depositor

   Laura Gunn

   Article

   Oh et al Mol Plant. 2024 Dec 2;17(12):1833-1849.

   Inserts

   Rubisco accumulation factor 1 (AT3G04550 Mustard Weed)
   Rubisco accumulation factor 2 (AT5G51110 Mustard Weed)
   RbcX2 (RbcX2 Mustard Weed)
   BSD2
   RbcX1
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   N terminus chloroplast transit peptide truncation

   Promoter

   T7

   Available Since

   June 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB-TR-hCAII

   Plasmid

   #232480

   Purpose

   Tetracycline inducible PiggyBac vector expressing human CAII gene including flag-tag (DYKDDDDK) on the 3' end

   Depositor

   Lukas Trantirek

   Article

   Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

   Insert

   Human carbonic anhydrase II (CA2 Human)
   

   Use

   Transposon system

   Tags

   DYKDDDDK (FLAG-tag)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-TetO

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHLsec hSOD1

   Plasmid

   #232481

   Purpose

   vector for transient transfection expressing human SOD1gene including flag-tag (DYKDDDDK) on the 3' end

   Depositor

   Lukas Trantirek

   Article

   Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1

   Insert

   Homo sapiens superoxide dismutase 1 (SOD1 Human)
   

   Use

   Tags

   DYKDDDDK (FLAG-tag)

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. YjdC-R82A_pET-14b

   Plasmid

   #233276

   Purpose

   Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 82 was substituted with alanine (R82A)

   Depositor

   Andrew Emili, Mohan Babu

   Article

   Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

   Insert

   YjdC (yjdC )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Arginine was replaced with Alanine at position 82…

   Promoter

   T7 promoter

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. YjdC-R64A_pET-14b

   Plasmid

   #233263

   Purpose

   Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 64 was substituted with alanine (R64A)

   Depositor

   Andrew Emili, Mohan Babu

   Article

   Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

   Insert

   YjdC (yjdC )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Arginine was replaced with Alanine at position 64…

   Promoter

   T7 promoter

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HemH-R212A_pET-14b

   Plasmid

   #233262

   Purpose

   Overexpress his-tag HemH protein with a site directed mutagenesis, arginine at position 212 was substituted with alanine (R121A)

   Depositor

   Andrew Emili, Mohan Babu

   Article

   Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

   Insert

   HemH (hemH )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Arginine was replaced with Alanine at position 21…

   Promoter

   T7 promoter

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p2X-KSR1-CA3-EGFP (2x-C-KSR)

   Plasmid

   #217756

   Purpose

   Fluorescent reporter for ceramide (putative, mammalian expression)

   Depositor

   Paula Nunes-Hasler

   Article

   Girik et al Int J Mol Sci. 2024 Mar 5;25(5):2996. do

   Insert

   Kinase suppressor of ras 1 CA3 domain (KSR1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Two tandem CA3 domain (aa 317-400) 1st linker GG…

   Promoter

   CMV

   Available Since

   June 10, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. K-to-R mutant NPRL3(1-569) in pLJM60, codon-optimized

    Plasmid

    #184563

    Purpose

    CMV-driven expression of an untagged mutant NPRL3 (core subunit of GATOR1) — codon-optimized sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.

    Depositor

    Kacper Rogala

    Article

    Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

    Insert

    C16orf35, CGTHBA, FFEVF3, HS-40, MARE, NPR3, RMD11 (NPRL3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    All lysines were mutated to arginines. Codon-opti…

    Promoter

    CMV

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. dCas9-p300 PHD-HAT

    Plasmid

    #179545

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of human p300 PHD-HAT domain (aa 1243-1664) (EP300 Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. dCas9-CBP RING-PHD-HAT

    Plasmid

    #179549

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP RING-PHD-HAT domain (aa 1191-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of human CBP RING-PHD-HAT domain (aa 1191-1701) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. dCas9-CBP PHD-HAT

    Plasmid

    #179548

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP PHD-HAT domain (aa 1279-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of human CBP PHD-HAT domain (aa 1279-1701) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. dCas9-p300 RING-PHD-HAT

    Plasmid

    #179546

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human p300 RING-PHD-HAT domain (aa 1155-1664) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of human p300 RING-PHD-HAT domain (aa 1155-1664) (EP300 Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLY9

    Plasmid

    #130907

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-2G6, and Ptet

    Available Since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLY59

    Plasmid

    #130927

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::MCP controlled by promoter J23106), and the reporter part (PpspA-2G6 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    sfgfp
    pspFΔHTH::MCP
    

    Use

    Synthetic Biology

    Tags

    ASV tag and MCP (MS2 coat protein)

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-2G6, and Ptet

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLY57

    Plasmid

    #130926

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA5B5 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA5B5, and Ptet

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLY56

    Plasmid

    #130925

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA4B4 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA4B4, and Ptet

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLY55

    Plasmid

    #130924

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA3B3, and Ptet

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLY53

    Plasmid

    #130911

    Purpose

    A CRISPR activation device with the necessary genes (dcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter J23106), and the reporter part (PpspA-LEA1B1 with sfgfp::ASV).

    Depositor

    Baojun Wang

    Article

    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:

    Inserts

    dcas9
    tetR
    pspFΔHTH::λN22plus
    sfgfp
    

    Use

    Synthetic Biology

    Tags

    ASV tag and pspFΔHTH::λN22plus

    Expression

    Bacterial

    Mutation

    Mutations D10A and H840A on WT cas9. Synonymous m…

    Promoter

    Anderson promoter: J23106, PpspA-LEA1B1, and Ptet

    Available Since

    Sept. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge