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pEGFP-ninein coiled-coil 1

Plasmid

#73521

Purpose

Encodes the first coiled-coil domain of mouse ninein with an EGFP tag at its N-terminus

Depositor

Michel Bornens

Article

Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

Insert

ninein coiled-coil 1 (Nin Mouse)

Tags

EGFP tag

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2016

Availability

Academic Institutions and Nonprofits only
pET28-MRPP3-myc-His

Plasmid

#67864

Purpose

bacterial expression of PRORP (KIAA0391), full coding sequence (mitoch. form) + C-term. myc-His tag

Depositor

Walter Rossmanith

Article

Holzmann et al Cell. 2008 Oct 31;135(3):462-74. doi: 10

Insert

MRPP3 (KIAA0391 Human)

Tags

His and myc

Expression

Bacterial

Mutation

Amino acid 46 of recombinant MRPP3 is preceded by…

Promoter

T7

Available Since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAP G116A

Plasmid

#123098

Purpose

Expresses 3xFLAG-GABARAP G116A in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Glycine 116 to Alanine

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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rat APOBEC1-EGFP

Plasmid

#112857

Purpose

plasmid for the expression of a rat APOBEC1-EGFP C-terminal fusion protein

Depositor

Silvestro Conticello

Article

Severi et al RNA Biol. 2015;12(4):389-97. doi: 10.108

Insert

apolipoprotein B mRNA editing enzyme, catalytic polypeptide 1 (Apobec1 Rat)

Expression

Mammalian

Promoter

CMV promoter

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 V5-DEST mir-1-1 reporter

Plasmid

#46646

Purpose

expression of query pri-miRNA

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir-1-1 reporter

Use

RNAi; Expression of query pri-mirna sequences in …

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pCIS-krPAM2

Plasmid

#25457

Depositor

Richard Mains

Article

Milgram et al J Cell Biol. 1992 May . 117(4):717-28.

Insert

PAM2 (Pam Rat)

Expression

Mammalian

Mutation

Exon A deleted (exon A extends from nt 1475 to n…

Available Since

July 13, 2010

Availability

Academic Institutions and Nonprofits only
EV-A71 3C protease; aliases: Enterovirus A71 3C protease, non cleavable C-term 6xHis tag

Plasmid

#204816

Purpose

Bacterial expresison of Enterovirus A71 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

EV A71 3C protease

Expression

Bacterial

Available Since

Jan. 17, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCF806-shABC1

Plasmid

#186713

Purpose

Expresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC1 = shARAF.381-shBRAF.2015-shCRAF.2446). The shABC2 set is better than shABC1.

Depositor

Christof Fellmann

Article

Amen et al Cell Rep Methods. 2022 Jun 21;2(7):10023

Insert

ARAF, BRAF, CRAF (BRAF, RAF1, ARAF Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-LC3A G120A

Plasmid

#123090

Purpose

Expresses 3xFLAG-LC3A G120A in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Glycine 120 to Alanine

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLV_PP1-mCitrine

Plasmid

#172472

Purpose

Mammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.

Depositor

Sean R Collins

Article

Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

Insert

prolactinSS-PP1-mCitrine (parapinopsina Zebrafish)

Use

Lentiviral

Tags

mCitrine and prolactin signal sequence (cleaved p…

Expression

Mammalian

Promoter

EF1alpha

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR223 GABARAPL2 G116

Plasmid

#123088

Purpose

Gateway entry clone encoding human GABARAPL2 G116

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Use

Gateway Cloning

Mutation

Deleted amino acid 117. Stop codon after G116

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-GABARAPL2 G116A

Plasmid

#123104

Purpose

Expresses 3xFLAG-GABARAPL2 G116A in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 2 (GABARAPL2 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Glycine 116 to Alanine

Promoter

CMV

Available Since

May 13, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Laccase2 Exons 1-3

Plasmid

#91799

Purpose

Drosophila laccase2 minigene plasmid that expresses both a linear RNA and a circular RNA

Depositor

Jeremy Wilusz

Article

Liang et al Mol Cell. 2017 Nov 9. pii: S1097-2765(17

Insert

Laccase2 (stw Fly)

Expression

Insect

Promoter

Metallothionein Promoter (pMT)

Available Since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV 3xFLAG-LC3A G120

Plasmid

#123091

Purpose

Expresses 3xFLAG-LC3A G120 in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Deleted amino acid 121. Stop codon after G120.

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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CJ29 (let-7 perfect site)

Plasmid

#17766

Depositor

David Bartel

Article

Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

Insert

let-7 perfect site (let-7 Fly)

Use

Luciferase; Drosophila metallothionein gene promo…

Tags

renilla luciferase

Expression

Insect

Available Since

April 15, 2008

Availability

Academic Institutions and Nonprofits only
pCMV 3xFLAG-GABARAPL1 G116

Plasmid

#123102

Purpose

Expresses 3xFLAG-GABARAPL1 G116 in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

Deleted amino acid 117. Stop codon after G116

Promoter

CMV

Available Since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-N-Myc_775-1102 USP7

Plasmid

#131250

Purpose

Mammalian expression of the USP7 UBL3-5 domains with an N-terminal Myc tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)

Tags

Myc

Expression

Mammalian

Mutation

Encodes USP7 UBL domains 3-5 (USP7 amino acids 77…

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Puro GFP-GABARAPL1 G116A

Plasmid

#123245

Purpose

Expression vector with PGK promoter for low expression of EGFP-GABARAPL1 G116A. For lentivirus production and stable transduction in mammalian cells.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Insert

Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Glycine 116 to Alanine

Promoter

PGK

Available Since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter mir16-1 wt

Plasmid

#46683

Purpose

Expresses wild-type miR-16-1 in mammalian cells

Depositor

David Bartel

Article

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

mir16-1 wt (MIR16-1 Human, Synthetic)

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pU6-mir451 EF1Alpha-puro-T2A-BFP

Plasmid

#164792

Purpose

Express miR-451 under the U6 promoter in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

microRNA 451a (MIR451A Human)
puro-T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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