We narrowed to 59,350 results for: cloning

1. pD(Gib)_3'beta

   Plasmid

   #223867

   Purpose

   4G-cloning donor for 3'-Gibson overhang, beta-sequence

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   3'beta sequence and spacer
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pD(Gib)_3'gamma

   Plasmid

   #223868

   Purpose

   4G-cloning donor for 3'-Gibson overhang, gamma-sequence

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   3'gamma sequence and spacer
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pD(Gib)_3'delta

   Plasmid

   #223869

   Purpose

   4G-cloning donor for 3'-Gibson overhang, delta-sequence

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   3'delta sequence and spacer
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. p663-UBC-MTS-miniTurbo-V5_IDG-K

   Plasmid

   #214456

   Purpose

   Gateway destination clone of MTS-miniTurbo (mitochondrial control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   MTS-miniTurbo-V5
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. p663-UBC-ER-miniTurbo-V5_IDG-K

   Plasmid

   #214452

   Purpose

   Gateway destination clone of ER-miniTurbo (endoplasmic reticulum control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   ER-miniTurbo-V5
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p663-UBC-MTS-TurboID-V5_IDG-K

   Plasmid

   #214457

   Purpose

   Gateway destination clone of MTS-TurboID (mitochondrial control) tagged with C-terminal V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   MTS-TurboID-V5
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGGW_SM_BglII

   Plasmid

   #49314

   Purpose

   Subcloning vector for Golden Gate-based site-directed mutagenesis; BglII and KpnI to release the insert

   Depositor

   Joachim Wittbrodt

   Article

   Kirchmaier et al PLoS One. 2013 Oct 7;8(10):e76117. doi:

   Type

   Empty backbone

   Use

   Cloning vector

   Available Since

   Sept. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-mRFP

   Plasmid

   #13032

   Purpose

   Mammalian expression vector for cloning your gene fused to mRFP

   Depositor

   Doug Golenbock

   Has Service

   Cloning Grade DNA

   Insert

   Monomer Red Fluorescent Protein
   

   Expression

   Mammalian

   Available Since

   Nov. 7, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-YFP

   Plasmid

   #13033

   Purpose

   Mammalian expression vector for cloning your gene fused to YFP

   Depositor

   Doug Golenbock

   Has Service

   Cloning Grade DNA

   Insert

   Yellow fluorescent protein
   

   Expression

   Mammalian

   Available Since

   Nov. 3, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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10. EF1a- gfp-2a-puro

    Plasmid

    #129443

    Purpose

    expression of GFP and Puro R

    Depositor

    Oskar Laur

    Insert

    gfp-puro
    

    Use

    Lentiviral

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCDH-CMV-mCherry-EF1 Hygro

    Plasmid

    #129440

    Purpose

    express mCherry

    Depositor

    Oskar Laur

    Insert

    mcherry
    

    Use

    Lentiviral

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28GSTlinker-LIC

    Plasmid

    #119970

    Purpose

    Structure determination and a long linker aiming to keep GST tag free in DNA binding screening experiments.

    Depositor

    Cheryl Arrowsmith

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Jan. 9, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. 60-p3E_INTRON-EmptyRNAi

    Plasmid

    #163380

    Purpose

    p3E-Vector (Gateway R2-L3) for Tol2-kit cloning. Vector for performing RNAi (gene silencing - knockdown) in zebrafish via cloning and expression of synthetic miR(s).

    Depositor

    Jean Giacomotto

    Type

    Empty backbone

    Use

    RNAi

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDH-UBC-LUC-EF1 Hygro

    Plasmid

    #129436

    Purpose

    express LUC

    Depositor

    Oskar Laur

    Insert

    LUC
    

    Use

    Lentiviral

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. R777-E211 Hs.RAC3

    Plasmid

    #70495

    Purpose

    Gateway ORF clone of human RAC3 [NM_005052.2] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RAC3 (RAC3 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pOT2-poly-cis

    Plasmid

    #141177

    Purpose

    To clone any gene or STTM sequence or artificial miRNA under CaMV 35S promoter

    Depositor

    Guiliang Tang

    Type

    Empty backbone

    Use

    RNAi and Synthetic Biology

    Expression

    Plant

    Promoter

    CaMV 35S promoter

    Available Since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. R777-E327 Hs.SPRY1

    Plasmid

    #70611

    Purpose

    Gateway ORF clone of human SPRY1 [NM_001258038.1] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    SPRY1 (SPRY1 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. R777-E253 Hs.RASSF10

    Plasmid

    #70537

    Purpose

    Gateway ORF clone of human RASSF10 [NM_001080521.2] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RASSF10 (RASSF10 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. R777-E357 Hs.VAV1

    Plasmid

    #70641

    Purpose

    Gateway ORF clone of human VAV1 [NM_005428.3] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    VAV1 (VAV1 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. R777-E249 Hs.RASGRP4

    Plasmid

    #70533

    Purpose

    Gateway ORF clone of human RASGRP4 [NM_001146202.1] with stop codon (for native or N-terminal fusions)

    Depositor

    Dominic Esposito

    Insert

    RASGRP4 (RASGRP4 Human)
    

    Use

    Gateway entry clone

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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