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pRDA_052

Plasmid

#136474

Purpose

Lentiviral expression of AsCas12a gRNA

Depositor

John Doench

Publication

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

Puromycin resistance

Use

Lentiviral and Synthetic Biology

Promoter

EF1a

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLX302_FOXA1-V5

Plasmid

#70090

Purpose

Expression of human FOXA1 with C-terminal V5 tag, puromycin selection marker, 3rd generation lentiviral vector

Depositor

William Hahn

Publication

Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

CMV

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Flag-hPIASy

Plasmid

#15208

Depositor

Ke Shuai

Publication

Liu et al Proc Natl Acad Sci U S A. 2001 Mar 13. 9

Insert

PIASy (PIAS4 Human)

Tags

Flag

Expression

Mammalian

Available since

Feb. 8, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a BFP

Plasmid

#31481

Depositor

Andrew Scharenberg

Publication

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Traffic Light Reporter 1.1 (Sce target) EF BFP

Use

Lentiviral

Mutation

mCherry contains M9S and M16L to reduce backgroun…

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pSKI015

Plasmid

#11570

Depositor

Detlef Weigel

Publication

Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

Insert

CaMV 35S enhancers

Use

Activation tagging

Expression

Plant

Available since

May 4, 2006

Availability

Academic Institutions and Nonprofits only

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His6-MBP-uTEV3

Plasmid

#135464

Purpose

Bacterial expression of uTEV3 (full-length) protease

Depositor

Alice Ting

Publication

Sanchez et al Nat Methods. 2019 Dec 9. pii: 10.1038/s4

Insert

His6-MBP-uTEV3

Tags

MBP, His6

Expression

Bacterial

Mutation

S219V mutation (improves stability) , I138T/S153N…

Promoter

T5

Available since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRsetB-PercevalHR

Plasmid

#49081

Purpose

Bacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

Depositor

Gary Yellen

Publication

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

PercevalHR

Tags

7xHis

Expression

Bacterial

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pHAGE-E-cadherin-RFP

Plasmid

#79603

Purpose

pHAGE lentiviral vector with dsRed driven by the human E-cadherin promoter. When used in combination with FUGW-d2GFP-ZEB1 3' UTR, comprises the Z-cad dual sensor. Use 2nd generation packaging system

Depositor

Jeffrey Rosen

Publication

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

CDH1 promoter region (CDH1 Human)

Use

Lentiviral

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223-PDGFRB

Plasmid

#23893

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDGFRB (PDGFRB Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pF155 pcDNA3.1(+)SOD1G93A

Plasmid

#26401

Depositor

Elizabeth Fisher

Publication

Stevens et al PLoS One. 2010 . 5(3):e9541.

Insert

SOD1 (SOD1 Human)

Expression

Mammalian

Mutation

G93A

Available since

Nov. 23, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pET21a-Traptavidin

Plasmid

#24753

Depositor

Mark Howarth

Publication

Chivers et al Nat Methods. 2010 May;7(5):391-3. doi: 1

Insert

Traptavidin

Tags

His-6

Expression

Bacterial

Mutation

Streptavidin with 2 amino acid mutations: S52G an…

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
pRDA_052

Plasmid

#136474

Purpose

Lentiviral expression of AsCas12a gRNA

Depositor

John Doench

Publication

DeWeirdt et al Nat Biotechnol. 2020 Jul 13. pii: 10.103

Insert

Puromycin resistance

Use

Lentiviral and Synthetic Biology

Promoter

EF1a

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

Enlarge
pCVL Traffic Light Reporter 1.1 (Sce target) Ef1a BFP

Plasmid

#31481

Depositor

Andrew Scharenberg

Publication

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

Traffic Light Reporter 1.1 (Sce target) EF BFP

Use

Lentiviral

Mutation

mCherry contains M9S and M16L to reduce backgroun…

Available since

Aug. 22, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

Enlarge
pcDNA3.1-Peredox-mCherry-NLS

Plasmid

#32384

Depositor

Gary Yellen

Publication

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

Peredox-mCherry-NLS

Tags

Nuclear Localization Seq

Expression

Mammalian

Promoter

CMV

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

Enlarge
pRsetB-PercevalHR

Plasmid

#49081

Purpose

Bacterial expression vector of N-terminal His-tagged PercevalHR, a fluorescent sensor of ATP-to-ADP ratio.

Depositor

Gary Yellen

Publication

Tantama et al Nat Commun. 2013;4:2550. doi: 10.1038/nc

Insert

PercevalHR

Tags

7xHis

Expression

Bacterial

Available since

Nov. 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBABE-puro LEF1

Plasmid

#27023

Depositor

Joan Massague

Publication

Nguyen et al Cell. 2009 Jul 10. 138(1):51-62.

Insert

LEF1 (LEF1 Human)

Use

Retroviral

Expression

Mammalian

Available since

Dec. 17, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pDONR223-PDGFRB

Plasmid

#23893

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDGFRB (PDGFRB Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

Enlarge
pET21a-Traptavidin

Plasmid

#24753

Depositor

Mark Howarth

Publication

Chivers et al Nat Methods. 2010 May;7(5):391-3. doi: 1

Insert

Traptavidin

Tags

His-6

Expression

Bacterial

Mutation

Streptavidin with 2 amino acid mutations: S52G an…

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

Enlarge
pcDNA3.1-Peredox-mCherry-NLS

Plasmid

#32384

Depositor

Gary Yellen

Publication

Hung et al Cell Metab. 2011 Oct 5;14(4):545-54.

Insert

Peredox-mCherry-NLS

Tags

Nuclear Localization Seq

Expression

Mammalian

Promoter

CMV

Available since

Sept. 27, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pJC175e

Plasmid

#79219

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Publication

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PPSP gIII, luxAB

Expression

Bacterial

Promoter

PPSP

Available since

April 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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