161,424 results

1. pU6-Cj-sgRNA

   Plasmid

   #89753

   Purpose

   U6 promoter driven expression of Cj-SgRNA cloned with BsmbI

   Depositor

   Seokjoong Kim

   Article

   Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

   Insert

   Cj-SgRNA
   

   Use

   Sgrna expression under u6 promoter

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   May 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. GLUT1-GFP

   Plasmid

   #89440

   Purpose

   GLUT1 construct

   Depositor

   Jeffrey Rathmell

   Article

   Wieman et al Biochem J. 2009 Mar 1;418(2):345-67. doi

   Insert

   GLUT1 (Slc2a1 Rat)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available since

   May 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. AiP1653 - pAAV-AiE2333m-minBG-SYFP2-WPRE-BGHpA

   Plasmid

   #214506

   Purpose

   AiE2333m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

   Depositor

   The Allen Institute for Brain Science, Bosiljka Tasic

   Article

   Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   minBG

   Available since

   Aug. 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYPQ230-RRV

   Plasmid

   #188543

   Purpose

   Gateway entry clone (attL1 & attR5) for LbCas12a-RRV mediated genome editing

   Depositor

   Yiping Qi

   Article

   Zhang et al Genome Biol. 2023 Apr 30;24(1):102. doi:

   Insert

   LbCas12a-RRV
   

   Use

   CRISPR; Gateway compatible lbcas12a-rrv entry clo…

   Tags

   NLS

   Expression

   Plant

   Mutation

   G146R, D156R, R182V

   Promoter

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBAD-LacI

   Plasmid

   #46394

   Purpose

   Overproduction of LacI

   Depositor

   Florian Hollfelder

   Article

   Gatti-Lafranconi et al Microb Cell Fact. 2013 Jul 8;12:67. doi:

   Insert

   LacI
   

   Use

   Tags

   6xHIS tag

   Expression

   Bacterial

   Mutation

   silent T to C mutation at position 19

   Promoter

   arabinose promoter

   Available since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDule2-para-aminoPhe

   Plasmid

   #85503

   Purpose

   Plasmid for incorporating the non-canonical amino acid para-aminoPhe with the Mj pAF synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Mehl et al J Am Chem Soc. 2003 Jan 29;125(4):935-9.

   Insert

   p-aminoPhe tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Use

   Tags

   None

   Expression

   Bacterial

   Mutation

   Y32T, E107T, D158P, I159L, L162A

   Promoter

   lpp (constitutive)

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMIG-hNFATc2

   Plasmid

   #74050

   Purpose

   retroviral expression plasmid for human NFATc2/C

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   human NFATc2, isoform C (NFATC2 Human)
   

   Use

   Retroviral

   Tags

   no tag

   Expression

   Mammalian

   Mutation

   silent mutation A1723C in the sequence of NM_1730…

   Promoter

   pMSCV-LTRs

   Available since

   June 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUC18/promEco/Lysostaphin

   Plasmid

   #125765

   Purpose

   Production of staphylolytic protein lysostaphin from a constitutive promoter

   Depositor

   Benedykt Wladyka

   Article

   Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

   Insert

   lysostaphin
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   sequence corresponding to the mature form protein

   Promoter

   pemIK-Sa1 promoter

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. CXCR7-Tango

   Plasmid

   #66265

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   CXCR7 (ACKR3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3

    Plasmid

    #73208

    Purpose

    Expresses dL5(E52D)-mCer3 fusion protein in mitochondria of mammalian cells. (MBIC5, dL5**, FAP)

    Depositor

    Marcel Bruchez

    Article

    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46

    Insert

    COXIV-COX8-dL5-2XG4S-mCer3 (COX8A Synthetic, Human)
    

    Use

    Tags

    The FAP and mCerulean3 are fused with 2 copies of…

    Expression

    Mammalian

    Mutation

    The dL5** FAP is E50D, L89S, also known as E52D/L…

    Promoter

    CMV

    Available since

    March 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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