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Venus-PTP1b

Plasmid

#234740

Purpose

Mammalian expression of endoplasmic reticulum marker Venus-PTPb

Depositor

Nevin Lambert

Article

Lan et al Traffic. 2012 Nov;13(11):1450-6. doi: 10

Insert

Venus-PTP1b

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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p133Tol2-Olactb:MCS, hsp70l-mcherry-CreERT2

Plasmid

#227775

Purpose

Expression of beta actin driven BFP with multiple cloning site at 3' end. Also contains heat-shock activated mCherry reporter and CreERT2.

Depositor

Bushra Raj

Article

Siniscalco et al Development. 2024 Nov 22:dev.203102. doi

Inserts

BFP-MCS
mCherry-t2A-CreERT2

Use

Zebrafish expression

Promoter

Olactb and hsp70l

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pEJS2697-CMV-Nme2SmuCas9-E932R

Plasmid

#228718

Purpose

Expresses Nme2Smu Cas9 nuclease with E932R mutation in mammalian cells

Depositor

Erik Sontheimer

Article

Bamidele et al bioRxiv [Preprint]. 2024 Nov 7:2024.10.3

Insert

Nme2SmuCas9-E932R nuclease

Use

CRISPR

Expression

Mammalian

Mutation

Nme2-Cas9, Smu PID, E932R

Promoter

CMV

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pJMP3667

Plasmid

#222353

Purpose

Expression vector with GFP expressed under PabstBR promoter, hygR marker, and replicates in A. baumannii and E. coli.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Insert

sfGFP

Expression

Bacterial

Promoter

PabstBR

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pJMP12049

Plasmid

#222355

Purpose

Tn7 integrative vector with GFP expressed under PabstBR promoter and aprR marker on Tn7 transposon.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Insert

sfGFP

Expression

Bacterial

Promoter

PabstBR

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pmEGFP-C1-Nir3-LNS2

Plasmid

#220087

Purpose

C-terminal PA binding domain of Nir3

Depositor

Gerry Hammond

Article

Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

Insert

PITPNM2 (PITPNM2 Human)

Tags

mEGFP-GGGGSHM

Expression

Mammalian

Mutation

amino acids 925-1209

Promoter

CMV

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pKH161_FLAG-APEX2-YIPF3 WT

Plasmid

#214980

Purpose

Flag-APEX2 tagged YIPF3

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2-YIPF3 (YIPF3 Human)

Use

Lentiviral

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKH152_PB_flag-LC3B_LDSmut_IRES-BFP

Plasmid

#214977

Purpose

Flag tagged LDS mutant LC3B

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-LC3B_mut (MAP1LC3B Human)

Expression

Mammalian

Mutation

LDS mutant

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pIRESneo-FLAG/HA-Ago2-Y529E

Plasmid

#215842

Purpose

Expression of AGO2-Y529E mutant

Depositor

Kumiko Ui-Tei

Article

Nishi et al Nucleic Acids Res. 2015 Nov 16;43(20):98

Insert

Human AGO2 (AGO2 Human)

Expression

Mammalian

Mutation

Ago2-Y529E

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

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mt TEFM

Plasmid

#205058

Purpose

TEFM protein expression

Depositor

Smita Patel

Article

Sultana et al J Biol Chem. 2017 Nov 3;292(44):18145-18

Insert

TEFM (TEFM Human)

Expression

Bacterial

Mutation

Codon optimized

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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LLP617_αGCN4-mCherry

Plasmid

#211785

Purpose

SunTag counterpart binding domain, aGCN4, fused to catalytically inert mCherry control, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

SnoopCatcher-DNMT3A

Tags

2xOLLAS

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect DNMT3A…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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LLP778_Traptavidin-p65HSF1-CO

Plasmid

#211771

Purpose

AviTag counterpart binding domain, Traptavidin, fused to transcriptional activator p65HSF1, with sfGFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

Traptavidin-p65HSF1

Tags

3xV5

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect p65HSF…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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LLP776_SpyCatcher-p65HSF1-CO

Plasmid

#211769

Purpose

SpyTag counterpart binding domain, SpyCatcher, fused to transcriptional activator p65HSF1, with GFP selection

Depositor

Ryan Lister

Article

Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.

Insert

SpyCatcher-p65HSF1

Tags

3xFLAG

Expression

Mammalian

Mutation

Last 300 bp codon-optimised (CO) to detect p65HSF…

Promoter

pEF1a and pSV40

Available Since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pSEVA83-GG

Plasmid

#208016

Purpose

for cloning homology arms, with GFP as counter selection marker

Depositor

Pablo Ivan Nikel

Article

Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

Insert

msfGFP

Available Since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

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mc2 mCherry EF1a

Plasmid

#206225

Purpose

For expressing cloned exons as circular RNA. Alternatively, for expressing cDNA (if the EcoRI-XhoI restriction fragment is also removed).

Depositor

Simon Conn, Brett Stringer

Article

Stringer et al bioRxiv. 2023 Nov 22:2023.11.21.568171.

Type

Empty backbone

Use

Synthetic Biology

Expression

Mammalian

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4_1G11F2-3 heavy chain

Plasmid

#198253

Purpose

recombinant expression of heavy chain of monoclonal anti-AMP antibody 1G11F2-3

Depositor

Aymelt Itzen

Article

Hopfner et al iScience. 2020 Nov 13;23(12):101800. doi

Insert

heavy chain of monoclonal anti-AMP antibody 1G11F2-3 (mouse IgG2b)

Tags

IL10 signal peptide

Expression

Mammalian

Promoter

CMV

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBZS-YP_009702000

Plasmid

#198379

Purpose

Express YP_009702000 in bacterial cells.

Depositor

Takashi Ito

Article

Miura et al BMC Biotechnol. 2022 Nov 4;22(1):33. doi

Insert

YP_009702000 (CVA-1_913R )

Expression

Bacterial

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCytERM-hfYFP

Plasmid

#186520

Purpose

Visualization of the ER in cells using hyperfolder YFP (compatible with OSER assay)

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

Hyperfolder YFP

Tags

Cytochrome p450 first 29 animo acids (Oryctolagus…

Expression

Mammalian

Mutation

mGreenLantern-F46L/C48S/V68L/A69M/C70V/K101E/T105…

Promoter

CMV

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pgRNA-backbone

Plasmid

#172517

Purpose

Yeast plasmid encoding a 2-kb gRNA-placeholder flanked by BsmBI/Esp3I restriction sites to facilitate gRNA insertion

Depositor

Leonid Kruglyak

Article

Schubert et al Elife. 2022 Nov 3;11. pii: 79525. doi: 1

Insert

gRNA cassette

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Nov. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pAB167

Plasmid

#101672

Purpose

Opto-T7RNAP*(563), equal expr.

Depositor

Mustafa Khammash

Article

Baumschlager et al ACS Synth Biol. 2017 Nov 17;6(11):2157-2

Insert

Opto-T7RNAP*(563), equal expr.

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 15, 2022

Availability

Academic Institutions and Nonprofits only

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