We narrowed to 19,553 results for: Mut

Showing: 5781 - 5800 of 19553 results

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2XMyc-LRRK2-RQ/TG

Plasmid

#25369

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Mutation

Threonine 1343 mutated to Glycine Arginine 139…

Available Since

June 29, 2010

Availability

Academic Institutions and Nonprofits only
pAdSh.U6.gRNAS1

Plasmid

#58252

Purpose

U6 promoter-driven single guide RNA complementary to the human AAVS1 locus

Depositor

Manuel Goncalves

Article

Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

Insert

U6.gRNAS1 cassette

Use

Adenoviral and CRISPR

Expression

Mammalian

Available Since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only

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pDGE463

Plasmid

#153234

Purpose

Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

Depositor

Sylvestre Marillonnet

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pDUP1

Plasmid

#223516

Purpose

inactive cogfp under control of Ptet, cogfp under control of Ptac

Depositor

Yolanda Schaerli

Article

Mihajlovic et al eLife. 2025 Apr 2;13:RP97216. doi: 10.75

Inserts

CoGFP
CoGFP inactivated

Tags

His tag

Expression

Bacterial

Mutation

anti-dimerization mutations S129G, 22 C154S, D156…

Promoter

Ptac, Ptac and Ptet, and Ptet

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223 PAK5

Plasmid

#60532

Purpose

Donor vector for human Pak5

Depositor

John Brognard

Article

Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

Insert

Pak5 (PAK5 Human)

Use

Gateway donor vector

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only
hGFAP R239C

Plasmid

#169476

Purpose

Single p.Arg239Cys(R239C) mutations was individually inserted by site-directed mutagenesis into the wild-type hGFAP

Depositor

Federico Herrera

Article

Letra-Vilela et al Experimental Results (2020), Volume 1, e

Insert

EGFP-hGFAP (GFAP Human)

Tags

EGFP

Expression

Mammalian

Mutation

Changed R 239 to C

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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hLOXL2-S300P

Plasmid

#200061

Purpose

LOXL2 mutant

Depositor

Lakshmi Santhanam

Article

Wang et al Commun Biol. 2023 Apr 7;6(1):375. doi: 1

Insert

human lysyl oxidase like 2 (LOXL2 Human)

Expression

Mammalian

Mutation

S300P mutation

Available Since

April 12, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-miR-34 MT

Plasmid

#78259

Purpose

psiCHECK2 dual luciferase reporter harboring a mutated miR-34 target element, cloned into the XhoI/NotI restriction sites, the 3'UTR of the Renilla Luciferase gene

Depositor

Joanne Weidhaas

Article

Salzman et al Nat Commun. 2016 Mar 21;7:10954. doi: 10

Insert

mutated miR-34 target site

Use

Luciferase; Microrna activity

Expression

Mammalian

Mutation

nucleotides 2-5 and 10-11 changed from CCGT to GG…

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)_mtZFN(-)2G

Plasmid

#62799

Purpose

mtZFN2G architecture bearing (-) FokI domain

Depositor

Michal Minczuk

Article

Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

Insert

mtZFN(-)2G

Tags

Internal FLAG tag immediately after F1B mitochond…

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(-)_mtZFN(+)2G

Plasmid

#62798

Purpose

mtZFN2G architecture bearing (+) FokI domain

Depositor

Michal Minczuk

Article

Gammage et al EMBO Mol Med. 2014 Apr;6(4):458-66. doi:

Insert

mtZFN(+)2G

Tags

Internal HA tag immediately after F1B mitochondri…

Expression

Mammalian

Promoter

CMV

Available Since

March 11, 2015

Availability

Academic Institutions and Nonprofits only

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pDGE479

Plasmid

#153270

Purpose

M4E shuttle vector containing the Solanum lycopersicum U3 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pDGE331

Plasmid

#153240

Purpose

M1E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pRCP

Plasmid

#195862

Purpose

Encodes chromogenic protein (CP). CP inducible with rhamnose.

Depositor

Gregory Phillips

Article

DeWolf et al J Microbiol Biol Educ. 2023 Oct 25;24(3)

Insert

Chromogenic Protein

Expression

Bacterial

Available Since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pET-FLAG-SpCas9-HF1

Plasmid

#126770

Purpose

Expression of increased fidelity SpCas9-HF1 in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

Insert

SpCas9-HF1

Use

CRISPR

Tags

3xFLAG, 6xHis, MBP, and NLS

Expression

Bacterial

Mutation

N497A, R661A, Q695A, Q926A

Promoter

T7

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pWL89A mmPing20

Plasmid

#145788

Purpose

mPing Yeast Transposition Assay

Depositor

Charles Nathan Hancock

Article

Johnson et al Plant Direct. 2021 Jan 19;5(1):e00300. d

Insert

mmPing20

Expression

Yeast

Mutation

T162C, T258A, T287C, T300A, T304A, T310A, G372A

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pHH29_32 series plasmid

Plasmid

#120901

Purpose

Also known as pHH29_32_HG27, one of the pHH29_32 series plasmids. The component plasmid of a unregulated or a regulated device

Depositor

Domitilla Del Vecchio

Article

Huang et al Nat Commun. 2018 Dec 21;9(1):5415. doi:

Inserts

Ec-TTL-P109
sRNA-A targeting sequence
RBS TIR=974 of GFPop1 gene
GFPop1
RBS TIR=6474 of ECF32mut gene
ECF32mut
T7 terminator
resource competitor

Use

Synthetic Biology

Expression

Bacterial

Available Since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT/TO-p97wt-mycStrep

Plasmid

#31837

Depositor

Hemmo Meyer

Article

Ritz et al Nat Cell Biol. 2011 Aug 7;13(9):1116-23.

Insert

p97

Tags

Strep and myc

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Oct. 17, 2011

Availability

Academic Institutions and Nonprofits only
XE241 Rat frizzled-1-GFP-CS2P+

Plasmid

#16817

Depositor

Randall Moon

Article

Kaykas et al Nat Cell Biol. 2004 Jan . 6(1):52-8.

Insert

frizzled-1 (Fzd1 Rat)

Use

Xenopus expression

Tags

GFP

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pLmoCascade-IL1RNcrRNA

Plasmid

#126487

Purpose

Encodes L.monocytogenes CRISPR Type I-B crRNA targeting IL1RN

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

Lmo IL1RN cr03

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-ExRai-AKAR(T/A)

Plasmid

#118406

Purpose

Negative-control mutant for ExRai-AKAR biosensor.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-AKAR(T/A)

Tags

6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Mutation

Contains Thr-to-Ala mutation in substrate sequenc…

Promoter

CMV

Available Since

April 25, 2019

Availability

Academic Institutions and Nonprofits only

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