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We narrowed to 62,288 results for: cloning

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Showing: 5781 - 5800 of 62288 results

  5. pDONR P4-P1R-mKate2

    Plasmid
    #48344
    Purpose
    Contributes the coding sequence for the mKate2 fluorescent protein as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Depositor
    Anna Planas, Tomas Santalucia
    Article
    Buj et al BMC Mol Biol. 2013 Aug 20;14(1):18. doi:
    Insert
    mKate2
    Use
    Gateway Cloning
    Mutation
    It contains a Kozak sequence but no stop codon.
    Promoter
    none
    Available Since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    48344-plasmid-map-sequence-id-70388

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  8. pOT2-poly-cis

    Plasmid
    #141177
    Purpose
    To clone any gene or STTM sequence or artificial miRNA under CaMV 35S promoter
    Depositor
    Guiliang Tang
    Type
    Empty backbone
    Use
    RNAi and Synthetic Biology
    Expression
    Plant
    Promoter
    CaMV 35S promoter
    Available Since
    Oct. 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    141177-plasmid-map-sequence-id-299765

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  9. pSPIH6

    Plasmid
    #190676
    Purpose
    E. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)
    Depositor
    John Vederas
    Article
    Lamer et al BMC Biotechnol. 2023 Apr 5;23(1):11. doi
    Inserts
    Small ubiquitin-like modifier
    Mxe GyrA intein with C-terminal chitin binding domain and His tag
    Tags
    Chitin binding domain, His tag, and N-terminal Hi…
    Expression
    Bacterial
    Mutation
    Added C-terminal his tag and None
    Promoter
    T7
    Available Since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    190676-plasmid-map-sequence-id-383624

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  12. pREVtre GFP

    Plasmid
    #128069
    Purpose
    Retroviral tet inducible EGFP
    Depositor
    Oskar Laur
    Insert
    tet inducible EGFP
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    no
    Available Since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    128069-plasmid-map-sequence-id-255185

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  13. FUGW ds red

    Plasmid
    #128067
    Purpose
    Lentiviral expression of dsRed Express2
    Depositor
    Oskar Laur
    Insert
    ds red
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    no
    Available Since
    Aug. 28, 2019
    Availability
    Academic Institutions and Nonprofits only
    128067-plasmid-map-sequence-id-255182

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  14. pCoofy42

    Plasmid
    #55190
    Purpose
    Pichia pastoris expression vector for parallel SLIC cloning. C-terminal tags can be added during SLIC.
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Tags
    His10-Stop-OneStrep-Stop-S-tag-Stop-CBP-HPC4-Stop…
    Expression
    Yeast
    Promoter
    AOX1
    Available Since
    July 28, 2014
    Availability
    Academic Institutions and Nonprofits only
    55190-plasmid-map-sequence-id-85572

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  15. pCMVlac-dirTopo-AU1-aGFP

    Plasmid
    #61971
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Available Since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61971-plasmid-map-sequence-id-101286

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  16. pCMVlac-dirTopo-P2A-aGFP

    Plasmid
    #61972
    Purpose
    A directional cloning vector of PCR-amplified cDNAs for mammalian expression
    Depositor
    Hiroshi Udo
    Inserts
    lacZ alpha
    EGFP
    Expression
    Mammalian
    Mutation
    It contains silent mutations
    Available Since
    Feb. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    61972-plasmid-map-sequence-id-101285

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Showing: 5781 - 5800 of 62288 results