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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 63,044 results for: cloning

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Showing: 5781 - 5800 of 63044 results

  1. pFHMSP-LIC-C

    Plasmid
    #26100
    Purpose
    Donor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    8x His tag and MKFLVNVALVFMVVYISYIYAAA (Honeybee …
    Expression
    Insect
    Promoter
    polyhedrin
    Available since
    Aug. 20, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles

  2. pShuttle-CMV-GFP U6promoter

    Plasmid
    #128049
    Purpose
    Adenoviral shuttle vector for shRNA expression from U6 promoter with GFP marker
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Use
    Adenoviral
    Expression
    Mammalian
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    128049-plasmid-map-sequence-id-255046

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  6. pCDH-EF1-IRES plum

    Plasmid
    #128072
    Purpose
    gfp was exchanged to plum
    Depositor
    Oskar Laur
    Insert
    plum
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    no
    Available since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128072-plasmid-map-sequence-id-255193

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  9. pelBHisTEV

    Plasmid
    #157741
    Purpose
    empty vector; TEV-protease-removable N-term PelB signal sequence +His10; potential C-term His6; T7lac promoter; Kan-R; avoids added residues in final protein since cloning uses BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Tags
    PelB signal sequence + His10-tag + TEV protease c…
    Expression
    Bacterial
    Promoter
    T7lac
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    157741-plasmid-map-sequence-id-334005

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  10. pFHMSP-LIC-N

    Plasmid
    #26095
    Purpose
    Donor vector to generate recombinant baculovirus by site-specific transposition into a baculovirus shuttle vector For use with Bac-to-Bac Expression System insect cells for secreted protein expression
    Depositor
    Cheryl Arrowsmith
    Type
    Empty backbone
    Use
    Baculovirus expression
    Tags
    His and TEV cleavage site
    Expression
    Insect
    Promoter
    polyhedrin
    Available since
    Sept. 15, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. Escherichia coli DH10B-MOB

    Bacterial strain
    #229395
    Purpose
    Escherichia coli DH10B DAP-auxotrophic strain containing an integrated RK2/RP4 mobilization vector to conjugate plasmids with an IncP origin of transfer (modified pTA-Mob 2.0, AddGene Plasmid #149662)
    Depositor
    Michael Springer
    Publication
    Soltysiak et al ACS Synth Biol. 2025 Apr 18;14(4):1173-1
    Bacterial resistance
    60 ug/mL of DAP
    Species
    Escherichia coli
    Available since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only

  12. pVL1393-6xMyc

    Plasmid
    #128035
    Purpose
    Baculovirus expression vector with C-terminal 6xMyc tag
    Depositor
    Oskar Laur
    Type
    Empty backbone
    Tags
    6xMyc tag
    Expression
    Insect
    Available since
    Nov. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    128035-plasmid-map-sequence-id-254860

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  15. pREXC3H

    Plasmid
    #47078
    Purpose
    FX cloning Lactococcus lactis expression vector (VBEx) with nisin promoter and C-terminal 3C protease cleavage site and 10x His tags
    Depositor
    Raimund Dutzler, Eric Geertsma
    Publication
    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.
    Type
    Empty backbone
    Use
    Lactococcus lactis expression
    Tags
    3C protease site (PreScission site) and decaHis (…
    Promoter
    NIS (nisin)
    Available since
    Jan. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    47078-plasmid-map-sequence-id-67104

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  16. pUC19_GZG

    Plasmid
    #117226
    Purpose
    shble flanked by an endogenous GAPDH promoter and terminator system
    Depositor
    Jackie Collier
    Insert
    shble
    Use
    Thraustochytrid expression
    Promoter
    GAPDH
    Available since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    117226-plasmid-map-sequence-id-224760

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  17. pT/BH

    Plasmid
    #26554
    Depositor
    Perry Hackett
    Insert
    pT/BH
    Expression
    Bacterial
    Available since
    Dec. 7, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  18. pT/HB

    Plasmid
    #26555
    Depositor
    Perry Hackett
    Insert
    pT/HB
    Expression
    Bacterial
    Available since
    Dec. 7, 2011
    Availability
    Academic Institutions and Nonprofits only

  20. plex.mcs sv40promoter-luc

    Plasmid
    #129429
    Purpose
    Luc expression under SV40 promoter
    Depositor
    Oskar Laur
    Insert
    LUC
    Use
    Lentiviral
    Available since
    March 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    129429-plasmid-map-sequence-id-256107

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Showing: 5781 - 5800 of 63044 results