We narrowed to 9,355 results for: pho;

1. pLz47_polyPro_CsoS2_Npeptide

   Plasmid

   #140845

   Purpose

   Consensus N*-peptide after polyproline II helix, N-terminal His- tag used as BLI bait, pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   polyproline helix with CsoS2 N-peptide
   

   Use

   Tags

   Hexahistidine tag and Strep-tag II

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBz110_CsoS2_CTD

   Plasmid

   #140844

   Purpose

   His-tagged CsoS2 C-terminal domain (CTD), pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   CsoS2 C-terminal domain (CTD)
   

   Use

   Tags

   Hexahistidine tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 1-594

   Promoter

   T7

   Available since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHCKan-T7-CBD-hGLY

   Plasmid

   #134940

   Purpose

   T7 based expression of human glycine acyltransferase with an N-terminal chitin binding domain

   Depositor

   Michael Lynch

   Article

   Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

   Insert

   human glycine acyltransferase 2 (GLYATL2 )
   

   Use

   Tags

   chitin binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   t7

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2+ rat MYO1D

   Plasmid

   #118419

   Purpose

   For mammalian expression of rat myosin 1D

   Depositor

   Michael Tsang

   Article

   Saydmohammed et al Nat Commun. 2018 Aug 23;9(1):3381. doi:

   Insert

   rat myosin 1D (Myo1d Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTH825-CEN-minRLuc/slowstaCFLuc

   Plasmid

   #115370

   Purpose

   Expresses translation elongation-controlled Renilla luciferase and translation initiation-controlled firefly luciferase

   Depositor

   Tobias von der Haar

   Inserts

   Gcn4 5'-UTR + Firefly Luciferase
   Renilla luciferase (codon minimised)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   All codons have been changed for the least favour…

   Promoter

   ADH1 and TDH3

   Available since

   March 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTriEX Tom20 mVenus BoPD

   Plasmid

   #114466

   Purpose

   Mammalian expression of mitochondrially targeted, mVenus labelled BoPD

   Depositor

   Andrew Woolley

   Article

   Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

   Insert

   BoPD
   

   Use

   Tags

   HIS, TEV and FLAG and TOM20 and mVenus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAGH50

   Plasmid

   #107254

   Purpose

   Vipp1-GFP-FKBP12 construct (spec cassette downstream) used to anchor away Vipp1 (sll0617) of Synecocystis PCC6803.

   Depositor

   Erin O'Shea

   Article

   Gutu et al Mol Microbiol. 2018 Jan 22. doi: 10.1111

   Insert

   vipp1-gfp-fkbp12
   

   Use

   Suicide vector for destined for double homologous…

   Tags

   GFP and FKBP12

   Expression

   Bacterial

   Mutation

   Promoter

   native

   Available since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFS345

   Plasmid

   #89349

   Purpose

   Beetle Luciferase C-term tagging vector

   Depositor

   Nick Rhind

   Article

   Keifenheim et al Curr Biol. 2017 May 22;27(10):1491-1497.

   Insert

   Beetle luciferase
   

   Use

   C-terminal tagging vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 18, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET21a - MPX

   Plasmid

   #89619

   Purpose

   [Edit] Expresses recombinant 6HIS tagged T. cruzi mitochondrial tryparedoxin peroxidase protein in E. coli upon IPTG induction

   Depositor

   Judy Lieberman

   Article

   Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

   Insert

   Mitocondrial Tryparedoxin peroxidase
   

   Use

   Tags

   6HIS and T7 tag (gene 10 leader)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET21a - EtfA

    Plasmid

    #89616

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein A in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    Electron transport flavoprotein alpha subunit
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET21a - EtfB

    Plasmid

    #89617

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein B in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    electron-transfer-flavoprotein, alpha polypeptide
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET21a - TDR

    Plasmid

    #89627

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi thiol dependent reductase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    thiol dependent reductase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET21a - NFDD

    Plasmid

    #89624

    Purpose

    Expresses recombinant 6HIS tagged T. cruzi NAD-FAD–dependent dehydrogenase in E. coli upon IPTG induction

    Depositor

    Judy Lieberman

    Article

    Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

    Insert

    NAD/FAD dependent dehydrogenase
    

    Use

    Tags

    6HIS and T7 tag (gene 10 leader)

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPD1R

    Plasmid

    #86969

    Purpose

    Expresses PCNA1 protein fused phosphite dehydrogenase and putidaredoxin reductase in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Sci Rep. 2015 Mar 3;5:8648. doi: 10.1038

    Insert

    PCNA1 fused to phosphite dehydrogenase and putidaredoxin reductase
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    E175A/A176R in PTDH

    Promoter

    T7 promoter

    Available since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pQCXIP-Laforin Myc 3CS (C250S,C266S,C278S)

    Plasmid

    #84661

    Purpose

    mammalian expression and/or retrovirus preparation

    Depositor

    Ayano Satoh

    Article

    Toyota et al Sciencematters

    Insert

    Laforin (EPM2A Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    Nov. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pQCXIP-Laforin Myc 3CS_new (C266S, C278S, C329S)

    Plasmid

    #84662

    Purpose

    mammalian expression and/or retrovirus preparation

    Depositor

    Ayano Satoh

    Article

    Toyota et al Sciencematters

    Insert

    Laforin (EPM2A Human)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    5'LTR

    Available since

    Nov. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3-myc-mCE

    Plasmid

    #82459

    Purpose

    Expression of myc epitope and murine mRNA Capping Enzyme in mammalian cells

    Depositor

    Daniel Schoenberg

    Article

    Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

    Insert

    mRNA capping enzyme (Rngtt Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. ARK1a

    Plasmid

    #81016

    Purpose

    E.coli BW25113 + JBEI-12056 (pBbA5c-MevTo-BBa1002-pTrc-MKco-PMKco, JBx_041105) + JBEI-9348 (pTrc99a-PMDsc-NudB, JBx_039365)

    Depositor

    Taek Soon Lee

    Article

    Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

    Inserts

    MevTo-BBa1002-pTrc-Mkco-PMKco
    PMD-NudB
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    LacUV5 and Trc promoter

    Available since

    Aug. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET24a_CDP-CHase

    Plasmid

    #73161

    Purpose

    Expresses in E. coli, Bacillus cereus NP_831800 CDP-CHase

    Depositor

    Sandra Gabelli, Maurice Bessman

    Article

    Duong-Ly et al J Bacteriol. 2011 Jul;193(13):3175-85. d

    Insert

    pET24a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pET24_BD3179

    Plasmid

    #73142

    Purpose

    Expresses in E.coli B. bacteriovorus Bd3179

    Depositor

    Sandra Gabelli

    Article

    de la Peña et al PLoS One. 2015 Nov 2;10(11):e0141716. do

    Insert

    Bd3179
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2016

    Availability

    Academic Institutions and Nonprofits only