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pLsCas13a-gRNA-1

Plasmid

#164865

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for LsCas13a in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

LsCas13a-gRNA-1

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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SMAD4 KO Neo

Plasmid

#16646

Depositor

Bert Vogelstein

Article

Zawel et al Proc Natl Acad Sci U S A. 2002 Mar 5. 99

Insert

Smad4 knock-out construct (SMAD4 Human)

Expression

Bacterial

Available Since

March 13, 2008

Availability

Academic Institutions and Nonprofits only
pMMRSF1-16

Plasmid

#20177

Depositor

Hideo Iwai

Article

Muona et al Chembiochem. 2008 Dec 15. 9(18):2958-61.

Insert

src homology domain 3 (CRK Human)

Tags

His and NpuDnaEC16

Expression

Bacterial

Available Since

Aug. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA-Evf2

Plasmid

#99478

Purpose

Mammalian expression of the Evf2 long non-coding RNA

Depositor

Jhumku Kohtz

Article

Feng et al Genes Dev. 2006 Jun 1;20(11):1470-84. Ep

Insert

Evf2

Expression

Mammalian

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCS2 xRor2-EGFP

Plasmid

#67163

Purpose

mammalian expression / in vitro transcription

Depositor

Alexandra Schambony

Article

Feike et al Biochem Biophys Res Commun. 2010 Oct 1;4

Insert

Tyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)

Use

In vitro transcription

Tags

EGFP

Expression

Mammalian

Available Since

July 16, 2015

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 N281

Plasmid

#17524

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal 281 amino acids

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pAS1_4xh7SKPylT_EF1_BsmBI_Intein_DD

Plasmid

#162810

Purpose

STELLA destination plasmid containing restriction sites for golden gate cloning. Suitable for amber suppression (4xMmaPylT cassette); for transient expression

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

BsmBI_Intein_DD

Use

Piggybac

Expression

Mammalian

Promoter

EF1alpha

Available Since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pBRIT Pax7 N391

Plasmid

#17525

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal 391 amino acids

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF2F12

Plasmid

#71060

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Chd3 (Chd3 Fly)

Use

Gateway entry vector

Available Since

Dec. 3, 2015

Availability

Academic Institutions and Nonprofits only
pTH736-CEN-staHIS3

Plasmid

#38228

Depositor

Tobias von der Haar

Article

Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

Insert

HIS3 (HIS3 Budding Yeast, Synthetic)

Tags

HA

Expression

Yeast

Promoter

TDH3 (=GPD)

Available Since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF2D09

Plasmid

#71017

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

MED15 (MED15 Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3D10

Plasmid

#71004

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

MED23 (MED23 Fly)

Use

Gateway entry vector

Available Since

Dec. 2, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1B02

Plasmid

#70782

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

MED9 (MED9 Fly)

Use

Gateway entry vector

Available Since

Nov. 24, 2015

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF3A03

Plasmid

#70957

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Hira (Hira Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
pAJ01

Plasmid

#14908

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

strongyloides stercoralis era-1 3' UTR

Expression

Worm

Available Since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pFOSGE_COF3F06

Plasmid

#70920

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

Orc4 (Orc4 Fly)

Use

Gateway entry vector

Available Since

Nov. 19, 2015

Availability

Academic Institutions and Nonprofits only
PE_3: Plux_lac_bD

Plasmid

#113604

Purpose

PE_3: Plux_lac_bD

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Plux_lac_bD

Expression

Bacterial

Promoter

Hybrid Promoter : Plux_lac_bD

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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PE_13: Plux_lac_tet_bD

Plasmid

#113594

Purpose

PE_13: Plux_lac_tet_bD

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter : Plux_lac_tet_bD

Expression

Bacterial

Promoter

Hybrid Promoter : Plux_lac_tet_bD

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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PE_27: Plas_lac_tet_eG::sfYFP

Plasmid

#104888

Purpose

PE_27: Plas_lac_tet_eG::sfYFP

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter Plas_lac_tet_eG

Expression

Bacterial

Promoter

Hybrid Promoter: Plas_lac_tet_eG

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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PE_28: Plux_lac_tet_eG::sfYFP

Plasmid

#104889

Purpose

PE_28: Plux_lac_tet_eG::sfYFP

Depositor

Matthew Bennett

Article

Chen et al Nat Commun. 2018 Jan 4;9(1):64. doi: 10.

Insert

Hybrid Promoter Plux_lac_tet_eG

Expression

Bacterial

Promoter

Plux_lac_tet_eG::sfYFP

Available Since

Jan. 15, 2019

Availability

Academic Institutions and Nonprofits only

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