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pcDNA3.1(+)-PKAcat(G1A)-mCherry

Plasmid

#181852

Purpose

mCherry-tagged PKA catalytic subunit lacking N-terminal myristoylation site; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

PKAcat-mCherry (PRKACA Human)

Tags

mCherry

Expression

Mammalian

Mutation

Glycine residue immediately after start site in P…

Promoter

CMV

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-SynaptoZip

Plasmid

#177318

Purpose

AAV expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by human Synapsin promoter

Depositor

Antonio Malgaroli

Article

Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

Insert

mCherry-SynaptoZip (Vamp2 Rat)

Use

AAV

Tags

Myc and mCherry

Expression

Mammalian

Promoter

hSyn

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-22-DExCon-mNeonGreen-R11a

Plasmid

#179897

Purpose

Donor with homologous arms for Rab11a to knock in DExCon-mNeonGreen module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11A, member RAS oncogene family (RAB11A Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Promoter

TRE3GS

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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JAM2_pLENTI-CAG-IRES-GFP

Plasmid

#176997

Purpose

Mammalian lentiviral expression vector encoding JAM2

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

JAM2 (JAM2 Human)

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-3exo-Tetra-com-CLCN5-sp-g1

Plasmid

#176236

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

Plasmid

#176237

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-Exo-tetra-com-hCLCN5-sp-g1

Plasmid

#176238

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pSIN-Gpi1

Plasmid

#175934

Purpose

knockout mouse Gpi1

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Gpi1 gRNA (Gpi1 Mouse)

Use

Retroviral

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pGMC00003

Plasmid

#166720

Purpose

sgRNA against mouse Kitl

Depositor

Raj Chari

Article

Rice et al Nat Commun. 2018 Nov 30;9(1):5099. doi:

Insert

Kitl (Kitl Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGMC00004

Plasmid

#166721

Purpose

sgRNA against mouse Kitl

Depositor

Raj Chari

Article

Rice et al Nat Commun. 2018 Nov 30;9(1):5099. doi:

Insert

Kitl (Kitl Mouse)

Use

CRISPR

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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PWPXLD-EGFR-P667A

Plasmid

#133750

Purpose

EGFR with mutation at proline 667 converted to alanine and tagged with HA cloned into PWPXLD plasmid

Depositor

Chay Kuo

Article

Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

Insert

Mutant P667A EGFR-HA (EGFR Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Mutation

proline 667 converted to alanine (Please see depo…

Promoter

EF1-alpha

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-f1(+)-ACTB-iTagRFP-T

Plasmid

#115915

Purpose

donor to insert iTagRFP-T at the ACTB locus in human cells

Depositor

Erik Sontheimer

Article

Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

Insert

ACTB-TagRFP-T (ACTB Human)

Use

Donor template

Tags

TagRFP-T

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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pKI-cjACTB-EGFP-3'short

Plasmid

#129749

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

ACTB (ACTB C. jacchus (common marmost))

Use

Marmoset targeting

Tags

EGFP

Available Since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pKI-cjACTB-EGFP-5'short

Plasmid

#129748

Purpose

Gene targeting in marmoset cells

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Sci Rep. 2019 Feb 6;9(1):1528. doi: 10.1

Insert

ACTB (ACTB C. jacchus (common marmost))

Use

Marmoset targeting

Tags

EGFP

Available Since

Oct. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLC-Flag-CSNK1A1 G40N-P2A-Blast

Plasmid

#123321

Purpose

Lentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Blast casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

changed Glycine 40 to Asparagine

Promoter

CMV

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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eGFP-PRM-3R

Plasmid

#112156

Purpose

Mammalian eGFP expression plasmid for PRM-3R.

Depositor

Michael Rosen

Article

Li et al Nature. 2012 Mar 7;483(7389):336-40. doi

Insert

PRM-3R (ABL1 Human)

Expression

Mammalian

Mutation

contains only the PRM domain of ABl1

Promoter

CMV

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR160

Plasmid

#106054

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR160; Opto-GPR160; E8)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR160 (GPR160 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR83

Plasmid

#106035

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR83; Opto-GPR83; D1)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR83 (GPR83 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S824E-S828E

Plasmid

#91999

Purpose

Expresses FLAG-HA-AGO2 (S824E-S828E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E, S828E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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MSCV-Puro-FH-AGO2-S824E-S831E

Plasmid

#92000

Purpose

Expresses FLAG-HA-AGO2 (S824E-S831E)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Retroviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

S824E, S831E

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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