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pAG414GPD-FvCYP722C2(pYL878)

Plasmid

#178299

Purpose

Expresses FvCYP722C2 from F. vesca in Saccharomyces cerevisiae. Centromeric TRP, PGPD-FvCYP722C2-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

FvCYP722C2

Expression

Yeast

Promoter

GAP

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET28b-trAtCCD8(pYL736)

Plasmid

#178287

Purpose

Expresses trAtCCD8 in E. coli. pET28b carrying t N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

trAtCCD8

Expression

Bacterial

Promoter

T7

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-OsCYP711A3(pYL761)

Plasmid

#178300

Purpose

Expresses OsCYP711A3 from O. sativa in Saccharomyces cerevisiae. Centromeric TRP, PGPD-OsCYP711A3-TCYC1

Depositor

Yanran Li

Article

Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

Insert

OsCYP711A3

Expression

Yeast

Promoter

GAP

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pUAST tdTomato mouse Rab35 WT

Plasmid

#53514

Depositor

Matthew Scott

Article

Zhang et al Science. 2009 Sep 4;325(5945):1250-4. do

Insert

Rab35 (Rab35 Mouse)

Tags

tdtomato

Expression

Insect

Promoter

GAL4

Available Since

June 3, 2014

Availability

Academic Institutions and Nonprofits only
KpAvs4

Plasmid

#188796

Purpose

Avs4 from Klebsiella pneumoniae UNMC_8078

Depositor

Feng Zhang

Article

Gao et al Science. 2022 Aug 12;377(6607):eabm4096.

Insert

KpAvs4

Expression

Bacterial

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAW212

Plasmid

#113234

Purpose

E. coli expression vector for untagged E. coli Cas1 + Cas2 with CRISPR array, leader GGTAG->CCATC

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

Cas1
Cas2
Leader-repeat

Expression

Bacterial

Mutation

GGTAG->CCATC

Available Since

Aug. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pFF749

Plasmid

#61452

Purpose

IPTG-inducible msd_kanR(ON)_dRT

Depositor

Timothy Lu

Article

Farzadfard et al Science. 2014 Nov 14;346(6211):1256272.

Insert

msd_kanR(ON)_dRT

Use

Synthetic Biology

Mutation

D197A D198A

Promoter

pLacO

Available Since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pBbB3a-GFP

Plasmid

#35346

Depositor

Jay Keasling

Article

Lee et al J Biol Eng. 2011 Sep 20;5:12.

Insert

GFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

ProS

Available Since

Feb. 20, 2012

Availability

Academic Institutions and Nonprofits only

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pWT004j

Plasmid

#107887

Purpose

R5 plasmid. Contains PBAD-EGFP-115-135insertion, T206G and is part of the CAMERA system for rewritable multi-event analog recording in bacteria

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PBAD-EGFP-115-135insertion, T206G

Expression

Bacterial

Mutation

insertion of nt 115-135, T206G nt change

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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ACOT7A

Plasmid

#42389

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Nicola Burgess-Brown

Insert

ACOT7A (ACOT7 Human)

Tags

His6-TEV

Expression

Bacterial

Mutation

See Comments

Available Since

March 18, 2013

Availability

Academic Institutions and Nonprofits only
RaCCR1_EYFP_pcDNA3.1

Plasmid

#159122

Purpose

This plasmid encodes RaCCR1 rhodopsin domain

Depositor

John Spudich

Article

Sineshchekov et al mBio. 2020 Apr 21;11(2). pii: mBio.00657

Insert

RaCCR1

Tags

EYFP

Expression

Mammalian

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a(+)/FaGH17A

Plasmid

#86462

Purpose

Expression of the Beta-1,3-Endo-Laminarinase FaGH17A in E.coli

Depositor

Jan-Hendrik Hehemann

Article

Becker et al Appl Environ Microbiol. 2017 Feb 17. pii

Insert

FaGH17A

Tags

His-Tag

Expression

Bacterial

Promoter

T7

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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MazF-K56A

Plasmid

#164274

Purpose

To express recombinant MazF K56A point mutant protein

Depositor

Guanzheng Luo

Article

Zhang et al Sci Adv. 2019 Jul 3;5(7):eaax0250. doi:

Insert

MazF

Expression

Bacterial

Mutation

K56A

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pNR186

Plasmid

#79552

Purpose

Output plasmid: contains 5-state register that regulates expression of GFP. Doesn't behave as expected.

Depositor

Timothy Lu

Article

Roquet et al Science. 2016 Jul 22;353(6297):aad8559.

Insert

GFPmut3b

Expression

Bacterial

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pJMP2480

Plasmid

#160080

Purpose

Z. mobilis CRISPRi, promoter C, empty guide

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Expression

Bacterial

Available Since

Nov. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19 gene 60 delta gap

Plasmid

#49091

Purpose

Template for generating gene 60 mRNA, coding gap deletion

Depositor

Nils Walter

Article

Todd et al RNA. 2013 May;19(5):685-700. doi: 10.126

Insert

Bacteriophage T4 gene 60

Mutation

Coding gap and coding gap STOP have been deleted.

Promoter

T7

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Sestrin2-E451A

Plasmid

#73674

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Tags

HA

Expression

Mammalian

Mutation

E451A changed Glutamate 451 to Alanine

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA 3.1(-) TRIM30D

Plasmid

#87084

Purpose

Murine homologues of human Trim5

Depositor

Jeremy Luban

Article

Lascano et al J Virol. 2015 Oct 14;90(1):308-16. doi:

Insert

TRIM30D (Trim30d Mouse)

Expression

Mammalian

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Sestrin2-L261A

Plasmid

#73672

Purpose

overexpression

Depositor

David Sabatini

Article

Wolfson et al Science. 2016 Jan 1;351(6268):43-8. doi:

Insert

Sesn2 (SESN2 Human)

Tags

HA

Expression

Mammalian

Mutation

L261A changed Leucine 261 to Alanine

Promoter

CMV

Available Since

March 30, 2016

Availability

Academic Institutions and Nonprofits only
pClodANL-FGF2YE

Plasmid

#107872

Purpose

Bacterial expression of FGF2phosphomimic-NanoLuc

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Insert

FGF2-phosphomimic

Use

Luciferase

Tags

NanoLuc

Expression

Bacterial

Mutation

Y82E

Promoter

pro D

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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