We narrowed to 13,494 results for: Mpl;

1. TDP-43_CTD_Q331K

   Plasmid

   #98673

   Purpose

   Expresses 6xHis-tagged C-terminal domain of TDP-43 with Q331K

   Depositor

   Nicolas Fawzi

   Article

   Conicella et al Structure. 2016 Sep 6;24(9):1537-49. doi

   Insert

   TDP-43 (TARDBP Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Q331K

   Available Since

   July 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRN3P-NLS-EGFP-deadBtgh-3NLS

   Plasmid

   #194470

   Purpose

   Encodes for catalytically inactive bacterial O-GlcNAcase BtGH84 fused to enhanced GFP and NLSs for effective nuclear localization, in backbone for T3 In Vitro Transcription.

   Depositor

   Mathieu Boulard

   Article

   Formichetti et al Dev Cell 10 Jan 2024

   Insert

   BtGH84
   

   Use

   For in vitro transcription from t3 promoter after…

   Tags

   SV40 Nuclear Localization Signal, SV40 Nuclear Lo…

   Mutation

   Aspartic Acid 242 (in Dennis et al.) changed to A…

   Available Since

   Jan. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. NSP6-FR-2Strep

   Plasmid

   #210317

   Purpose

   Expresses SARS-CoV-2 NSP6 in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   SARS-CoV-2 NSP6 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)
   

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Many synonymous changes due to codon optimization

   Available Since

   Jan. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. EGFP-Caveolin-3

   Plasmid

   #68396

   Purpose

   Fluorescently tagged Caveolin-3 for expression in mammalian cells

   Depositor

   Rob Parton

   Article

   Luetterforst et al J Cell Biol. 1999 Jun 28;145(7):1443-59.

   Insert

   Cav3 (Cav3 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-CaMKII-jGCaMP7f-WPRE

   Plasmid

   #203848

   Purpose

   Expression of GCaMP7f in CaMKII expressing cells

   Depositor

   Ivo Spiegel

   Article

   Cohen-Kashi Malina et al Neuron. 2021 Jul 7;109(13):2150-2164.e5.

   Insert

   jGCaMP7f
   

   Use

   AAV

   Available Since

   Aug. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pENTR_L1-Turbo-YFP-NLS-STOP-L2

   Plasmid

   #127361

   Purpose

   gateway entry vector for expressing nuclear TurboID-YFP under a promoter of choice

   Depositor

   Dominique Bergmann

   Article

   Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

   Insert

   TurboID (BirA mutant)
   

   Use

   Gateway entry vector

   Tags

   GS linker, NLS, V5, and YFP

   Mutation

   Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

   Promoter

   no promoter

   Available Since

   Sept. 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pUC-GFP-HS

   Plasmid

   #133305

   Purpose

   "Burdensome" GFP expressing plasmid carrying hok/sok toxin-antitoxin for plasmid stabilisation

   Depositor

   Chris Barnes

   Article

   Fedorec et al iScience. 2019 Apr 26;14:323-334. doi: 1

   Insert

   hok/sok toxin-antitoxin cassette
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. bArr1-V5

   Plasmid

   #201492

   Purpose

   Expression of the beta-arrestin1 containing a C-terminal V5 tag.

   Depositor

   Patrick Giguère

   Article

   Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

   Insert

   bArr1-V5 (ARRB1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMBEC2

   Plasmid

   #187596

   Purpose

   Base editing plasmid, constitutively expressing cytosine base editor and cas6. Contains GFP, flanked by BsaI restriction sites to introduce spacer and gRNAs. Kanamycin resistance

   Depositor

   Pablo Ivan Nikel

   Article

   Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

   Insert

   nCas9-BEC-UGI; cas6f, gfp
   

   Expression

   Bacterial

   Promoter

   trc

   Available Since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDuRCC-K

    Plasmid

    #81193

    Purpose

    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below., Please view…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CAS9PBKS

    Plasmid

    #68371

    Purpose

    CbAkt promoter driven Cas9-2A-GFP (from px458)

    Depositor

    Eric-Paul Bennett

    Article

    Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

    Insert

    Cas9
    

    Tags

    2A-EGFP

    Expression

    Mammalian

    Promoter

    CbAkt

    Available Since

    Feb. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-EF1a-FLEX-GT

    Plasmid

    #26198

    Depositor

    Edward Callaway

    Article

    Wall et al Proc Natl Acad Sci U S A. 2010 Dec 14;10

    Insert

    Enhanced green fluorescent protein, TVA (eGFP TVA is a mouse codon optimized version of an avian gene., Mouse)
    

    Use

    AAV and Cre/Lox

    Mutation

    TVA mammalian codon optimized

    Available Since

    Sept. 16, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pPEP-TEV cNup98 (1-498)

    Plasmid

    #38037

    Depositor

    Roderick Lim

    Article

    Kowalczyk et al Nat Nanotechnol. 2011 Jun 19;6(7):433-8.

    Insert

    Nucleoporin 98 (NUP98 Human)
    

    Tags

    His-tag, 3Cysteine

    Expression

    Bacterial

    Mutation

    amino acids 1-498

    Promoter

    T7

    Available Since

    Sept. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSR660

    Plasmid

    #65216

    Purpose

    Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-8 cap gene in Sf9 insect cells

    Depositor

    Robert Kotin

    Article

    Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

    Inserts

    modified AAV-2 rep78
    modified AAV-8 cap
    

    Use

    AAV

    Expression

    Insect

    Promoter

    P10 and polyhedrin

    Available Since

    June 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBabe-Puro-REDD1-HA

    Plasmid

    #133550

    Purpose

    Expresses HA tagged REDD1 in Mammalian cells

    Depositor

    James Brugarolas

    Article

    Vega-Rubin-de-Celis et al Biochemistry. 2010 Mar 23;49(11):2491-50

    Insert

    Regulated in development and DNA damage response 1 (REDD1) (DDIT4 Human)
    

    Use

    Retroviral

    Tags

    HA tag

    Expression

    Mammalian

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTag-BFP-C-h-Rab5a-c-Myc

    Plasmid

    #79801

    Purpose

    Express TagBFP labeled human Rab5a (GTPase located on early endosomal membranes).

    Depositor

    James Johnson

    Article

    Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

    Insert

    Rab5a (RAB5A Human)
    

    Tags

    TagBFP and myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pIDS-RESTwt full-length

    Plasmid

    #109161

    Purpose

    Encodes human full-length REST/NRSF to be expressed in a baculovirus/insect cell expression system

    Depositor

    Monika Golas

    Article

    Inui et al Protein Sci. 2017 May;26(5):997-1011. do

    Insert

    human full-length REST/NRSF (REST Human)
    

    Expression

    Insect

    Promoter

    p10

    Available Since

    Jan. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. qTAG-N-Solo-mStayGold-TJP1

    Plasmid

    #227300

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the TJP1 locus. For tight junction visualization. To be co-transfected with sgRNA plasmid px330-TJP1 (Addgene #227299)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TJP1 Homology Arms flanking a mStayGold Tag (TJP1 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAT003

    Plasmid

    #171628

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting TRAC and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-TRAC
    mNeon
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    CAG and EF1-a

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEGFP-GIT1

    Plasmid

    #15226

    Depositor

    Rick Horwitz

    Article

    Manabe et al J Cell Sci. 2002 Apr 1. 115(Pt 7):1497-5

    Insert

    G protein coupled receptor kinase-interacting protein 1 (GIT1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    L425V, E476K and L583P

    Available Since

    Sept. 13, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge