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pUC57-GFP-SFPQ-RT

Plasmid

#97090

Purpose

Encodes a HR repair template for knock-in of an EGFP insert that fused to the 5'-end of human SFPQ gene, without disturbing its promoter. Best used with px330-GFP-SFPQ vector.

Depositor

Archa Fox

Article

Li et al RNA. 2017 Mar 21. pii: rna.059477.116. d

Insert

EGFP-SFPQ-RT (SFPQ Human)

Use

CRISPR

Available Since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Arf1

Plasmid

#10830

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf1 (ARF1 Bovine)

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3 HA Arf1

Plasmid

#10830

Depositor

Thomas Roberts

Article

Furman et al J Biol Chem. 2002 Mar 8. 277(10):7962-9.

Insert

Arf1 (ARF1 Bovine)

Tags

HA

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only
pCAGGS_cAMPFIRE-M

Plasmid

#182284

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-M (cAMP sensor)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS_cAMPFIRE-M

Plasmid

#182284

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-M under a CAG promotor w/ WPRE translation enhancer.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-M (cAMP sensor)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pet28a-His6-Keap1

Plasmid

#62454

Purpose

His6 tagged Keap1 for E.coli expression

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Tag

Expression

Bacterial

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pet28a-His6-Keap1

Plasmid

#62454

Purpose

His6 tagged Keap1 for E.coli expression

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Tag

Expression

Bacterial

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pET15TV-L-AldA WT

Plasmid

#187976

Purpose

Expresses human aldolase A

Depositor

Audrey Lamb

Article

Fenton et al Protein Sci. 2022 Feb;31(2):357-370. doi

Insert

AldA WT (ALDOA Human)

Tags

Hexahistag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pET15TV-L-AldA WT

Plasmid

#187976

Purpose

Expresses human aldolase A

Depositor

Audrey Lamb

Article

Fenton et al Protein Sci. 2022 Feb;31(2):357-370. doi

Insert

AldA WT (ALDOA Human)

Tags

Hexahistag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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PZac2.1 hSyn1-Lck-GFP

Plasmid

#177333

Purpose

Expresses GFP on the plasma membrane of neurons via an Lck localization sequence

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Lck-GFP

Use

AAV

Tags

GFP

Expression

Bacterial

Promoter

hSyn1

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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PZac2.1 hSyn1-Lck-GFP

Plasmid

#177333

Purpose

Expresses GFP on the plasma membrane of neurons via an Lck localization sequence

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Lck-GFP

Use

AAV

Tags

GFP

Expression

Bacterial

Promoter

hSyn1

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEJS613_pTetR-P2A-BFPnls/sgTelo

Plasmid

#108649

Purpose

Telomere-targeting Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Telomere-targeting sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS613_pTetR-P2A-BFPnls/sgTelo

Plasmid

#108649

Purpose

Telomere-targeting Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Gao et al Nat Methods. 2018 Jun;15(6):433-436. doi

Inserts

Telomere-targeting sgRNA
TetR-P2A-BFP

Use

CRISPR and Lentiviral

Tags

No

Expression

Mammalian

Promoter

U6 and hPGK

Available Since

May 9, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS_cAMPFIRE-L-R279E

Plasmid

#182286

Purpose

Mammalian expression of cAMPFIRE-L-R279E (non-cAMP-binding) under a CAG promotor w/ WPRE translation enhancer.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS_cAMPFIRE-L-R279E

Plasmid

#182286

Purpose

Mammalian expression of cAMPFIRE-L-R279E (non-cAMP-binding) under a CAG promotor w/ WPRE translation enhancer.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-L-R279E (cAMP-deficient cAMPFIRE-L)

Expression

Mammalian

Available Since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144-CMV2-mir1

Plasmid

#164634

Purpose

Express miR-144 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

mir144 (MIR144 Human)
mir-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pBI-CMV1-mir144-CMV2-mir1

Plasmid

#164634

Purpose

Express miR-144 and miR-1-1 in mammalian cells

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

mir144 (MIR144 Human)
mir-1-1 (MIR1-1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MEKK1 WT

Plasmid

#12181

Depositor

Gary Johnson

Article

Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

Insert

MEKK1 (Map3k1 Mouse)

Tags

His and Xpress

Expression

Mammalian

Mutation

V1467L

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3 MEKK1 WT

Plasmid

#12181

Depositor

Gary Johnson

Article

Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

Insert

MEKK1 (Map3k1 Mouse)

Tags

His and Xpress

Expression

Mammalian

Mutation

V1467L

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only

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pET21a-cys-His6-OtUBD

Plasmid

#190091

Purpose

Expresses OtUBD with a non-cleavable N-terminal 6xHis tag as well as an engineered N-terminal cysteine in E. coli

Depositor

Mark Hochstrasser

Article

Zhang et al PLoS Biol. 2022 Jun 30;20(6):e3001501. d

Insert

OtUBD

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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