We narrowed to 8,011 results for: ALP

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pET21a - EtfA

Plasmid

#89616

Purpose

Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein A in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

Electron transport flavoprotein alpha subunit

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a - EtfB

Plasmid

#89617

Purpose

Expresses recombinant 6HIS tagged T. cruzi electron transfer flavoprotein B in E. coli upon IPTG induction

Depositor

Judy Lieberman

Article

Dotiwala et al Nat Med. 2016 Feb;22(2):210-6. doi: 10.1

Insert

electron-transfer-flavoprotein, alpha polypeptide

Tags

6HIS and T7 tag (gene 10 leader)

Expression

Bacterial

Available Since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO ChETA-EYFP

Plasmid

#26968

Purpose

AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activation

Depositor

Karl Deisseroth

Article

Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

Has Service

AAV1, AAV5, and AAV9

Insert

hChR2(E123T/H134R)-EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Mutation

E123T, H134R

Promoter

EF1alpha

Available Since

Jan. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pLV-REPORT(EF1a no GFP) mnuctdTomato

Plasmid

#172342

Purpose

mnuctdTomato

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

monomeric nuclear Tomato
HygR

Use

Lentiviral and Synthetic Biology

Tags

SV40 NLS

Expression

Mammalian

Promoter

EF1alpha

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAM/CBA-eGFP-miRaSyn(human)x3-WPRE-bGHpA

Plasmid

#194247

Purpose

Expresses 3 miRNAs targeting human alpha-Synuclein

Depositor

Michael J Fox Foundation MJFF

Insert

miRaSynuclein(human) (SNCA Human)

Use

AAV and RNAi

Promoter

CAG

Available Since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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SGTA-EGFP

Plasmid

#210373

Purpose

Expresses SGTA fused with EGFP in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SGTA (SGTA Human)

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pMpGWB113

Plasmid

#68567

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pEFIRES-P-mCherry-HPos

Plasmid

#87159

Purpose

Fluorescent marker of lipid droplets (LDs)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

HPos

Tags

mCherry

Expression

Mammalian

Promoter

EF-1-alpha

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pLVE-eGFP

Plasmid

#52581

Purpose

Lentiviral transfer vector driving expression of eGFP in mammalian cells

Depositor

Charles Gersbach

Article

Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

Insert

eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

EF-1alpha

Available Since

Jan. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pYESir2-puro

Plasmid

#1769

Depositor

Bob Weinberg

Article

Vaziri et al Cell 2001 Oct 19;107(2):149-59.

Insert

Sir2 (SIRT1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

June 3, 2005

Availability

Academic Institutions and Nonprofits only

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pK-myc-Par3b

Plasmid

#19388

Depositor

Ian Macara

Article

Joberty et al Nat Cell Biol 2000 Aug;2(8):531-9.

Insert

Partitioning-defective protein 3b (PARD3 Human)

Tags

Myc

Expression

Mammalian

Mutation

Full length, isoform b.

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only

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PERK 1: PERK.WT.9E10.pCDNA

Plasmid

#21814

Depositor

David Ron

Article

Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

Insert

PERK (Eif2ak3 Mouse)

Tags

Myc (9E10)

Expression

Mammalian

Available Since

March 29, 2010

Availability

Academic Institutions and Nonprofits only

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pMpGWB308

Plasmid

#68636

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pET12a-PAPI

Plasmid

#19961

Depositor

Jonathan Tait

Article

Wood et al Blood. 1996 Sep 1. 88(5):1873-80.

Insert

Annexin V (ANXA5 Human)

Expression

Bacterial

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag Jnk2a2

Plasmid

#13755

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2a2 (MAPK9 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

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pMSP1E3D1_D73C

Plasmid

#39328

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E3D1_D73C (APOA1 synthetic gene)

Tags

6xHis

Expression

Bacterial

Mutation

cys mutant of MSP1E3D1

Promoter

T7

Available Since

Sept. 11, 2012

Availability

Academic Institutions and Nonprofits only
pEGFP-Akt

Plasmid

#39531

Depositor

Julian Downward

Article

Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

Insert

Akt (Akt1 Mouse)

Tags

6G linker and EGFP

Expression

Mammalian

Available Since

Aug. 15, 2012

Availability

Academic Institutions and Nonprofits only
pBad-mCherry-XL (R125K)

Plasmid

#188054

Purpose

Expresses His-tagged, Arabinose-inducible mCherry-XL (R125K) in bacteria

Depositor

Ralph Jimenez

Article

Mukherjee et al J Phys Chem B. 2022 Jun 16. doi: 10.1021

Insert

mCherry-XL (R125K)

Tags

6xHis

Expression

Bacterial

Mutation

differs from mCherry by R125K, W143S, I161V, Q163…

Promoter

araBAD

Available Since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pc DNA FRT TO- APP695

Plasmid

#114193

Purpose

Creation of a stable cell line expressing APP mutation (AD in vitro study)

Depositor

Aleksandra Radenovic

Insert

Amyloid-Precursor-Protein (APP Human)

Expression

Mammalian

Mutation

wild type splice variant 695: 695AA (2085bp, lack…

Promoter

CMV

Available Since

Oct. 24, 2018

Availability

Academic Institutions and Nonprofits only

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mApple-TNFa-RUSH

Plasmid

#166902

Purpose

RUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-mApple

Depositor

Jennifer Lippincott-Schwartz

Article

Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

Insert

mApple

Expression

Mammalian

Available Since

April 14, 2021

Availability

Academic Institutions and Nonprofits only

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