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We narrowed to 12,101 results for: VARS

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Showing: 5881 - 5900 of 12101 results

  7. pCMV-RR-A3A-N57G

    Plasmid
    #193654
    Purpose
    Expression of base editor RR-A3A-N57G in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Article
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Insert
    RR-dLbCas12a-hA3A-N57G, EGFP
    Expression
    Mammalian
    Mutation
    dLbCas12a-G532R/K595R, hAPOBEC3A-N57G
    Promoter
    CMV
    Available Since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193654-plasmid-map-sequence-id-422067

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  8. pCMV-RR-A3A-Y130F

    Plasmid
    #193655
    Purpose
    Expression of base editor RR-A3A-Y130F in mammalian cells for base editing, along with non-fused EGFP fluorescence
    Depositor
    Liangxue Lai
    Article
    Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:
    Insert
    RR-dLbCas12a-hA3A-Y130F, EGFP
    Expression
    Mammalian
    Mutation
    dLbCas12a-G532R/K595R, hAPOBEC3A-Y130F
    Promoter
    CMV
    Available Since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    193655-plasmid-map-sequence-id-422124

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  9. pCMV6-AN-DDK_S172A_M173A RAD23A

    Plasmid
    #201574
    Purpose
    Expresses a variant of human RAD23A containing mutations S172A and M173A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23A
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    UV excision repair protein RAD23 homolog A (RAD23A Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Contains mutations S172A and M173A
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201574-plasmid-map-sequence-id-395069

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  13. pCMV6-AN-DDK_C344A_F354A RAD23A

    Plasmid
    #201579
    Purpose
    Expresses a variant of human RAD23A containing mutations C344A and F354A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23A
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1
    Insert
    UV excision repair protein RAD23 homolog A (RAD23A Human)
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Contains mutations C344A and F354A
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    201579-plasmid-map-sequence-id-395063

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  14. HypaR-SpCas9

    Plasmid
    #126757
    Purpose
    Expression plasmid for human codon-optimized increased fidelity Hypa2SpCas9 (without U6-sgRNA coding sequence, with silent mutations)
    Depositor
    Ervin Welker
    Article
    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:
    Insert
    Hypa2SpCas9
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    R661A, N692A, M694A, Q695A, H698A
    Promoter
    CBh
    Available Since
    Sept. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    126757-plasmid-map-sequence-id-412372

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  16. pUC19-π-EB1_EF1α-mCherry

    Plasmid
    #197429
    Purpose
    Homology-directed repair template targeting EF1α-based π-element to MAPRE1 exon 5
    Depositor
    Torsten Wittmann
    Article
    Dema et al Elife. 2023 Jan 30;12:e84143. doi: 10.75
    Insert
    EF1α-based π-element with HDR templates flanking MAPRE1 exon 5 insertion site (MAPRE1 Human)
    Use
    CRISPR and Synthetic Biology
    Tags
    GCN4 leucine zipper, LOV2, Zdk1, and mCherry
    Available Since
    June 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    197429-plasmid-map-sequence-id-388078

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  19. pGADT7-δ

    Plasmid
    #197259
    Purpose
    Expression of GAL4 transcriptional activation domain (AD)-AP-3 δ fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Insert
    AP-3 δ (AP3D1 Human)
    Tags
    GAL4 transcriptional activation domain (AD) fragm…
    Expression
    Yeast
    Mutation
    Val 549 Ile substitution; silent substitutions in…
    Promoter
    ADH1
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197259-plasmid-map-sequence-id-387016

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  20. pBridge-tyrosinase.σ3A

    Plasmid
    #197513
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-3 σ3A fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-3 σ3A
    Tags
    GAL4-DNA binding domain fragment, HA tag, and nuc…
    Expression
    Yeast
    Mutation
    silent substitution in codon 2 of tyrosinase tail…
    Promoter
    ADH1 and MET25
    Available Since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197513-plasmid-map-sequence-id-388877

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Showing: 5881 - 5900 of 12101 results