We narrowed to 18,492 results for: REV

1. pET_His6-MBP-TEV-Cas6-RT-Cas1_WT

   Plasmid

   #186274

   Purpose

   Expresses Thiomicrospira Cas6-RT-Cas1 in E. coli

   Depositor

   Jennifer Doudna

   Article

   Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

   Insert

   Thiomicropira Cas6-RT-Cas1
   

   Tags

   His6-MBP

   Expression

   Bacterial

   Available Since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. DpbCas12e

   Plasmid

   #188495

   Purpose

   Expresses FLAG tagged DpbCas12e

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas12e
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. PlmCas12e

   Plasmid

   #188496

   Purpose

   Expresses FLAG tagged PlmCas12e

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas12e
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. DpbdCas12e KRAB

   Plasmid

   #188505

   Purpose

   Expresses FLAG tagged DpbdCas12e KRAB

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12e
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D672A/E769A/D935A

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. Cas12j3

   Plasmid

   #188497

   Purpose

   Expresses FLAG tagged Cas12j3

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas12j3
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pPpT4- HTA1prom-TIR1-FLAG

   Plasmid

   #189725

   Purpose

   Enables expression of OsTIR1 from the HTA1 promoter, used to implement the AID system in Komagataella phaffii (original name pLLpLL001)

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

   Insert

   OsTIR1
   

   Tags

   FLAG

   Expression

   Yeast

   Promoter

   HTA1

   Available Since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPpT4- TEF2prom-TIR1-FLAG

   Plasmid

   #189726

   Purpose

   Enables expression of OsTIR1 from the TEF2 promoter, used to implement the AID system in Komagataella phaffii (original name pLL002)

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Lehmayer et al iScience. 2022 Aug 6;25(9):104888. doi:

   Insert

   OsTIR1
   

   Tags

   FLAG

   Expression

   Yeast

   Promoter

   TEF2

   Available Since

   Sept. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pILGFP4D42

   Plasmid

   #185840

   Purpose

   Testing the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

   Depositor

   Claudia Vickers

   Article

   Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

   Insert

   PURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pIALD2E

   Plasmid

   #185866

   Purpose

   Knocking out ALD6

   Depositor

   Claudia Vickers

   Article

   Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

   Insert

   ALD(-125, 40)- ALD6(1054, 1749)
   

   Expression

   Yeast

   Mutation

   None

   Available Since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pILGFP10CF71

    Plasmid

    #185860

    Purpose

    Testing the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pILGFP10CF72 (B9)

    Plasmid

    #185858

    Purpose

    Testing the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M5(5*PZ4)>(BamHI)yEGFP>TURA3
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pILGFP10CF72

    Plasmid

    #185857

    Purpose

    Testing the SkGAL2 promoter inserted with 4 PZ4 elements using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M4(4*PZ4)>(BamHI)yEGFP>TURA3
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pILGFP10E1B

    Plasmid

    #185856

    Purpose

    Testing the SkGAL2 promoter inserted with 3 PZ4 elements using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M2(3*PZ4)>(BamHI)yEGFP>TURA3
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pILGFP10E1A

    Plasmid

    #185855

    Purpose

    Testing the SkGAL2 promoter inserted with 2 PZ4 elements using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2M1(2*PZ4)> (BamHI)yEGFP>TURA3
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pILGFP3A2 (1)

    Plasmid

    #185851

    Purpose

    Testing the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCfB9358(VII-1_Markerfree_BackBone)

    Plasmid

    #161599

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VII-1, (Chr VII: 438509..438490)

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCfB9359(VIII-1_Markerfree_BackBone)

    Plasmid

    #161600

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site VIII-1, (Chr VIII: 293615..293634)

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCfB9361(XIII-1_Markerfree_BackBone)

    Plasmid

    #161602

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XIII-1, (Chr XIII: 306804..306823)

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCfB9363(XVI-1_Markerfree_BackBone)

    Plasmid

    #161604

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XVI-1, (Chr XVI: 406267..406286)

    Depositor

    Irina Borodina

    Article

    Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. KBB439

    Plasmid

    #185093

    Purpose

    NMA111-GFP wild type under control of GAL1 promoter (complements nma111 deletion)

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    GAL1::NMA111-GFP

    Promoter

    GAL1

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. KBB462

    Plasmid

    #185095

    Purpose

    NMA111-GFP with NLS1 and NLS2 mutated under control of GAL1 promoter

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    GAL1::nma111Dnls1Dnls2-GFP

    Promoter

    GAL1

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. KBB394

    Plasmid

    #185086

    Purpose

    NMA111 with both nuclear localization signals mutated to alanines fused to GFP (mutagenesis of KBB280)

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Nma111 KKR 9-11 AAA; KRK 28-30 AAA

    Available Since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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23. KBB106

    Plasmid

    #185065

    Purpose

    Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion including C-terminal half of Nup1 C-terminal FG domain

    Depositor

    Ken Belanger

    Article

    Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

    Insert

    NUP1
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Nup1 C-terminal region, truncated so C-terminal h…

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pTS2373-Tier1-PhCMV-Gal4

    Plasmid

    #169581

    Purpose

    Tier-1 vector encoding PhCMV-driven Gal4 DNA binding domain (PhCMV-Gal4-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    regulatory protein GAL4
    

    Expression

    Mammalian

    Promoter

    PhCMV

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. pnEA-NpM-Scp20_18-49-GB1_AF

    Plasmid

    #148720

    Purpose

    Bacterial Expression of Scp20_18-49

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

    Insert

    Scp20_18-49 (CAF20 Budding Yeast)
    

    Expression

    Bacterial

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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26. pnEA-NpM-ScEap1p_91-150_AF

    Plasmid

    #148710

    Purpose

    Bacterial Expression of ScEap1p_91-150

    Depositor

    Elisa Izaurralde

    Article

    Gruner et al Nucleic Acids Res. 2018 Jul 27;46(13):68

    Insert

    ScEap1p_91-150 (EAP1 Budding Yeast)
    

    Expression

    Bacterial

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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27. pnYC-NpM-DmPAN3_349-790-P652G_T

    Plasmid

    #147570

    Purpose

    Bacterial Expression of DmPAN3_349-790-P652G

    Depositor

    Elisa Izaurralde

    Article

    Christie et al Mol Cell. 2013 Aug 8;51(3):360-73. doi:

    Insert

    DmPAN3_349-790-P652G (CG11486 Fly)
    

    Expression

    Bacterial

    Available Since

    March 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. Rp51a_10bp 5'SS_No BP or pBP_pUC18

    Plasmid

    #100989

    Purpose

    Rp51a with a tGGTAtGTta->AGGTAAGTAT 5'SS mutant with potential to form 10bps with the U1 snRNA. Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation

    Depositor

    Aaron Hoskins

    Article

    Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

    Insert

    RP51A (RPS17A Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    tGGTAtGTta->AGGTAAGTAT 5'SS mutant, TACTA…

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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29. Rp51a_SUS1 5'ss_No_BP_pBP-pUC18

    Plasmid

    #100990

    Purpose

    Contains model Rp51a with a GGTAtGT->tGTAtGa 5'SS mutant (SUS1 5'SS sequence). Also contains TACTAAC->GTTAGTG BP mutataion and a TACAAAC->GTTTGTG pseudo BP mutation

    Depositor

    Aaron Hoskins

    Article

    Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

    Insert

    RP51A (RPS17A Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    GGTAtGT->tGTAtGa 5'SS mutant, TACTAAC->…

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pMC8b-XI1up

    Plasmid

    #176166

    Purpose

    yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-1

    Depositor

    Florian David

    Article

    Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

    Insert

    5' homology for integration site XI-1
    

    Use

    Synthetic Biology

    Available Since

    Feb. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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