We narrowed to 66,557 results for: des.1
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Plasmid Kit#1000000054PurposeA collection that combines Golden Gate with Multisite Gateway cloning for assembly of complex fusion proteins or transgenesis constructs.DepositorApplicationCloning and Synthetic BiologyVector TypeBacterial Expression, Eukaryotic Expression, Mammalian Expression, Zebrafish ExpressionCloning TypeGolden GateAvailable SinceSept. 10, 2014AvailabilityAcademic Institutions and Nonprofits only
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pMSCV-Hygromycin-TTF-1
Plasmid#119173PurposeRetroviral mammalian expression vector containing wt-human TTF-1 cDNADepositorAvailable SinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDONR223-NEK2
Plasmid#23658DepositorInsertNEK2 (NEK2 Human)
UseGateway CloningMutationInsert encodes truncated protein relative to NP_0…Available SinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
AW01_pU.CAG.eSpCas9(1.1).rBGpA
Plasmid#199255PurposeExpression construct encoding the high specificity eSpCas9(1.1) nucleaseDepositorInsertHigh specificity eSpCas9(1.1) nuclease
UseCRISPRTags3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianMutationK848A K1003A R1060APromoterCAG promoterAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
XLone-BSD SPI1 P2A mCherry
Plasmid#140031PurposeTunable and temporal expression control of SPI1 and mCherryDepositorAvailable SinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_ManII-SBP-mCherry
Plasmid#65253Purposesynchronize trafficking of ManII from the ER (RUSH system)DepositorInsertStreptavidin-KDEL and truncated Mannosidase II
TagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianMutationcontains only amino acids 1 to 116 of ManIIPromoterCMVAvailable SinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
GAPDH
Plasmid#155622PurposeFor use in RBP tethering screenDepositorAvailable SinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_CTTN-1/1
Plasmid#91550PurposeProtein expression and purification of human SH3 domain construct CTTN-1/1DepositorInsertCTTN-1/1 (CTTN Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBI-CMV1-mir374b-CMV2-mir1
Plasmid#164672PurposeExpress miR-374b and miR-1-1 in mammalian cellsDepositorAvailable SinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSCV-LMP1-IRES-mCherry
Plasmid#220351PurposeExpresses EBV latent gene LMP1 in mammalian cellsDepositorAvailable SinceJune 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFlag-CMV2-WBP1
Plasmid#27477DepositorAvailable SinceMarch 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEM rat islet1 (phoII) [TJ#1]
Plasmid#16268DepositorInsertislet1 (Isl1 Rat)
ExpressionBacterialAvailable SinceFeb. 4, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2CR-QUASM1:GCaMP7s-NLS-P2A-mKate2-NLS
Plasmid#241915PurposeZebrafish transgenesis plasmid encoding GCaMP7s-NLS and mKate2-NES separated by the P2A peptide, driven by the QUASM1 enhancer, and including a cmlc2:mKate2 cardiac transgenesis markerDepositorInsertQUASM1 enhancer driving GCaMP7s-NLS and mKate2-NLS
UseZebrafish tol2 transgenesisPromoterQUASM1Available SinceOct. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
Plasmid#159838PurposeThree-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arraysDepositorInsertpDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
ExpressionWormMutationNot applicableAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
Desmoplakin II donor only control (FL-based no force control)
Plasmid#118722PurposeThe donor (YPet(short)) only control for the no force control of the FL-based human desmoplakin II tension sensor provides the donor only lifetime used in lifetime measurements to determine FRET.DepositorInserthuman Desmoplakin II (1-1353)-[YPet(short)-FL-mCherry(Y72L)] (DSP Human)
UseRetroviralTagsYPet(short)-FL-mCherry(Y72L)ExpressionMammalianMutationtruncation after aa1353; Y72L mutation in mCherry…PromoterCMVAvailable SinceDec. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Dest47- R5A-K5A N23 C11orf83-GFP
Plasmid#65846PurposeMammalian expression of the mutated N terminal part (R5A-K5A N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)DepositorAvailable SinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Dest47- R5D-K6D N23 C11orf83-GFP
Plasmid#65847PurposeMammalian expression of the mutated N terminal part (R5D-K5D N23, transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein (C-ter)DepositorAvailable SinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT-DEST EVC2ala7-TEV-YFP-Flag
Plasmid#41013DepositorInsertEllis van Creveld syndrome 2 (EVC2 Mouse)
TagsTEV-YFP-FlagExpressionMammalianMutationchanged amino acids 1189-1192 to AlanineAvailable SinceNov. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT-DEST EVC2ala5-TEV-YFP-Flag
Plasmid#41012DepositorInsertEllis van Creveld syndrome 2 (EVC2 Mouse)
TagsTEV-YFP-FlagExpressionMammalianMutationchanged amino acids 1185-1188 to AlanineAvailable SinceNov. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT-DEST EVC2ala4-TEV-YFP-Flag
Plasmid#41011DepositorInsertEllis van Creveld syndrome 2 (EVC2 Mouse)
TagsTEV-YFP-FlagExpressionMammalianMutationchanged amino acids 1183-1186 to AlanineAvailable SinceNov. 15, 2012AvailabilityAcademic Institutions and Nonprofits only