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pCEP4_OCT4_T234A_S235A

Plasmid

#40630

Depositor

James Thomson

Article

Brumbaugh et al Proc Natl Acad Sci U S A. 2012 May 8;109

Insert

POU5F1_S234A_T235A (POU5F1 Human)

Expression

Mammalian

Mutation

S234A_T235A

Promoter

EF1-alpha

Available Since

July 26, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-CLASP2 512-650 9xS/A

Plasmid

#24410

Depositor

Torsten Wittmann

Article

Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

Insert

CLASP2 (512-650) 9xS/A (CLASP2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

Nonphosphorylatable CLASP2 deletion mutant that c…

Available Since

April 23, 2010

Availability

Academic Institutions and Nonprofits only

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AAV_Efs_hSpCas9_NLS_FLAG-SV40

Plasmid

#97307

Purpose

AAV vector for encoding a human codon-optimized SpCas9 driven by EFs promoter

Depositor

Hui Yang

Article

Yao et al Cell Res. 2017 May 19. doi: 10.1038/cr.2

Insert

humanized S. pyogenes Cas9

Use

AAV and Mouse Targeting

Tags

Flag

Expression

Mammalian

Promoter

EFS

Available Since

Aug. 2, 2017

Availability

Academic Institutions and Nonprofits only

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Lenti-sgLkb1/Cre

Plasmid

#66894

Purpose

Expresses an Lkb1-targeting gRNA and Cre-recombinase

Depositor

Monte Winslow

Article

Chiou et al Genes Dev. 2015 Jul 15;29(14):1576-85. d

Insert

sgLkb1

Use

CRISPR, Cre/Lox, Lentiviral, and Mouse Targeting

Expression

Mammalian

Promoter

U6

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-H-RAN-GFP1

Plasmid

#106418

Purpose

H-RAN-GFP1 (RANbody GFP1::Sm-HAtag)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

H-RAN-GFP1

Tags

HA, His, and Multiple HA

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGIG-Sema7A-ΔSEMA

Plasmid

#190647

Purpose

Expresses Mouse Sema7A with SEMA domain deleted

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema7A (Sema7a Mouse)

Expression

Mammalian

Mutation

SEMA Domain Deleted

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGIG-Sema7A-ΔIG

Plasmid

#190648

Purpose

Expresses Mouse Sema7A with IG Domain Deleted

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema7A (Sema7a Mouse)

Expression

Mammalian

Mutation

IG Domain Deleted

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGIG-Sema7A-ΔPSI

Plasmid

#190649

Purpose

Expresses Mouse Sema7A with PSI domain deleted

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema7A (Sema7a Mouse)

Expression

Mammalian

Mutation

PSI Domain Deleted

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-HA-Sema7A

Plasmid

#190644

Purpose

Expresses Mouse Sema7A with HA Tag

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema7A (Sema7a Mouse)

Tags

HA

Expression

Mammalian

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pET-15b-aδNb072

Plasmid

#204627

Purpose

Expresses the anti-IgD nanobody 072 (aδNb072) in the bacterial periplasm. aδNb072 is an orthosteric inhibitor of human anti-Phl p 7 antibodies of clone 102.1F10.

Depositor

James McDonnell

Article

Vester et al Antibodies (Basel). 2023 Nov 16;12(4):75

Insert

aδNb072

Tags

His6 and TEV protease cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pSEVA424::tsi5

Plasmid

#192960

Purpose

Expresses the protein Tsi51-76 for biological function studies in a broad-spectrum of organisms

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

tsi5

Expression

Bacterial

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET29a(+)::D1141A

Plasmid

#192963

Purpose

Test the activity of the putative aspartyl protease motif DPXGL-(18)-DPXGL

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

Tse5_D1141A

Tags

9xhis + TEV tag

Expression

Bacterial

Mutation

D1141A

Available Since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pET29a(+)::9xhis-Tse5

Plasmid

#192962

Purpose

Heterologous expression of Tse5 in E. coli Lemo21 cells for purification of Tse5-CT

Depositor

David Albesa-Jové

Article

Gonzalez-Magana et al Commun Biol. 2022 Nov 5;5(1):1189. doi:

Insert

Tse5

Tags

9xhis + TEV tag

Expression

Bacterial

Available Since

Dec. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-H2BmGreenLantern

Plasmid

#177332

Purpose

To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.

Depositor

Murray Blackmore, Gregory Petsko, Pantelis Tsoulfas

Article

Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

Insert

H2B (Hist1h2bq Rat)

Use

AAV

Tags

mGreenLantern

Expression

Mammalian

Mutation

This fusion protein was optimized to the Human co…

Promoter

CAG

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pNH605-HSE-YFP

Plasmid

#172131

Purpose

Fluorescent reporter for Hsf1 activity

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

4xHSE-YFP

Use

Vector for genomic integration into the yeast gen…

Expression

Yeast

Promoter

CYC1 core promotor with 4 tandem repeats of the H…

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMSR0

Plasmid

#161984

Purpose

Allele-Coupled Exchange for chromosomal manipulation of Clostridioides difficile ribotype 027 and other ribotypes. Uses inducible expression of CD2517.1 toxin as a counter-selection marker

Depositor

Olga Soutourina

Article

Peltier et al Commun Biol. 2020 Nov 27;3(1):718. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Oct. 11, 2021

Availability

Academic Institutions and Nonprofits only

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RAPpGEX

Plasmid

#162241

Purpose

Expresses LDL-receptor associated protein (RAP), full length in mammalian cells

Depositor

Andreas Faissner

Article

Hennen et al J Biol Chem. 2013 Jun 7;288(23):16538-45

Insert

lrpap1 (Lrpap1 Mouse)

Tags

GST-tag

Expression

Mammalian

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pYX233-DHFR

Plasmid

#163761

Purpose

Gal-inducible yeast expression of cytosolic DHFR

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

DHFR (Dhfr Mouse)

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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PLX306 iCre tetO tomato rtTA

Plasmid

#158050

Purpose

lenti-viral construct with Cre recombinase and doxycycline inducible expression of tdTomato

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

tdTomato

Tags

none

Expression

Mammalian

Promoter

tetO promoter

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pRRLSIN.cPPT.PGK-GFP-PTS1.WPRE

Plasmid

#133282

Purpose

Lentiviral expression of a peroxisomal eGFP

Depositor

Etienne Sokal

Article

Demaret et al Histochem Cell Biol. 2020 Mar 2. pii: 10

Insert

Peroxisome targeting signal 1

Use

Lentiviral

Tags

Peroxisome targeting signal 1

Expression

Mammalian

Promoter

None

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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