We narrowed to 17,423 results for: tes

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pSEVA221AID-T7RNAP

Plasmid

#167977

Purpose

Expresses fusion protein AID-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Publication

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

AID-T7RNAP

Tags

T7 tag and T7RNAP

Expression

Bacterial

Promoter

TetR-Ptet

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pBS NL4-3 envFS eGFP PR-D25A

Plasmid

#101334

Purpose

pBS NL4-3 envFS eGFP with mutation that disrupts Protease catalytic activity

Depositor

Jeremy Luban

Publication

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3 envFS

Use

Lentiviral

Mutation

Protease D25A

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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TAL-L1-1.3-KRAB

Plasmid

#103034

Purpose

[Edit] Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by KRAB domain

Depositor

Maria-Elena Torres-Padilla

Publication

Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

Insert

TAL-L1-1.3-KRAB

Use

T3 in vitro transcription for expression in mamma…

Tags

HA and KRAB

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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TAL-L1-2.4-VP64

Plasmid

#103039

Purpose

Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by VP64 activator

Depositor

Maria-Elena Torres-Padilla

Publication

Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

Insert

TAL-L1-2.4-VP64

Use

T3 in vitro transcription for expression in mamma…

Tags

HA and VP64

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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TAL-L1-1.3-VP64

Plasmid

#103033

Purpose

Plasmid for IvT reaction with T3. Encodes LINE-1 specific TALE sequence followed by VP64 activator

Depositor

Maria-Elena Torres-Padilla

Publication

Jachowicz et al Nat Genet. 2017 Aug 28. doi: 10.1038/ng.

Insert

TAL-L1-1.3-VP64

Use

T3 in vitro transcription for expression in mamma…

Tags

HA and VP64

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pPBC-G6s

Plasmid

#62812

Purpose

pPBC-G6s expresses PiggyBac inverted terminal repeat-flanked GCaMP6s under the control of the CAG promoter.

Depositor

Karen Wilcox

Publication

Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

Insert

ITR-CAG-GCaMP6s-ITR

Expression

Bacterial and Mammalian

Promoter

CAG

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CRY2-CreN

Plasmid

#75268

Purpose

The second half of the photo-activatable cre recombinase

Depositor

Michael Bruchas

Publication

Schindler et al Sci Rep. 2015 Sep 9;5:13627. doi: 10.103

Insert

second half of the cre recombinase fused to CRY2 (cryptochrome 2) that has absorbed blue light (Kennedy et al., 2010)

Use

AAV

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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IVT-nCas9(H840A)

Plasmid

#207455

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Publication

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

H840A

Expression

Bacterial

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pHY002_Strep_Avi_hsDCAF15

Plasmid

#174479

Purpose

Expresses DCAF15 in insect cells

Depositor

Eric Fischer

Publication

Faust et al Nat Chem Biol. 2020 Jan;16(1):7-14. doi:

Insert

DCAF 15 (DCAF15 Human)

Tags

StrepII-Avi-TEV

Expression

Insect

Available since

Sept. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1E

Plasmid

#160442

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1 chromoprotein in E. coli

Depositor

Anthony Forster

Publication

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pmiRA1-MYC

Plasmid

#141121

Purpose

Lentiviral overexpression of MYC in mammalian cells.

Depositor

Jianjun Chen

Publication

Weng et al Cell Stem Cell. 2018 Feb 1;22(2):191-205

Insert

MYC (MYC Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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Flag-Rap1b V12

Plasmid

#118323

Purpose

Expression of bovine Rap1b

Depositor

Philip Stork

Publication

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

Rap1b V12 (RAP1B Bovine)

Tags

Flag

Expression

Mammalian

Mutation

G12V

Promoter

CMV

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pICH51288

Plasmid

#50269

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (double), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pENTR-Arl13b1

Plasmid

#40870

Depositor

Tamara Caspary

Publication

Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

Insert

Arl13b (Arl13b Mouse)

Use

Gateway Cloning

Available since

Oct. 29, 2012

Availability

Academic Institutions and Nonprofits only
pLNC Wnt-3

Plasmid

#17993

Depositor

Jan Kitajewski

Publication

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-3 (WNT3 Human)

Use

Retroviral

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pEGFP_mEGFP-GluA1c

Plasmid

#114136

Purpose

Mammalian expression of the cytosolic C terminus of GluA1 N-terminally tagged by mEGFP.

Depositor

Haining Zhong

Publication

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mEGFP tagged GluA1 C-terminus

Expression

Mammalian

Available since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-T7-hAIM2-EGFP

Plasmid

#51608

Purpose

Express T7 and GFP-tagged hAIM2 in mammalian cells

Depositor

Emad Alnemri

Publication

Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

Insert

AIM2 (AIM2 Human)

Use

Retroviral

Tags

EGFP and T7

Expression

Mammalian

Promoter

MESV

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only

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pEGFP-NMHC-IIA-S1916A

Plasmid

#101040

Purpose

Expresses GFP-MYH9 construct (human myosin IIA) carrying mutation of serine 1916 to alanine, driven by CMV promoter. Thus inhibits PKC phosphorylation of the tail. Also carries S1915A mutation.

Depositor

Tom Egelhoff

Publication

Rai et al J Biol Chem. 2017 Feb 24;292(8):3099-311

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Non-muscle myosin IIA heavy chain (MYH9 Human)

Tags

GFP

Expression

Mammalian

Mutation

S1916A, S1917A

Promoter

CMV

Available since

Nov. 22, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Cry2 mCherry GRK2 ct in pcDNA3.1

Plasmid

#64212

Purpose

mCherry fused between Cry2 and GRK2ct to study cell migration

Depositor

Narasimhan Gautam

Publication

O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

Cry2 PHR (CRY2 Mustard Weed)
GRK2 ct (GRK2 Human)

Tags

mcherry

Expression

Mammalian

Promoter

CMV and cmv

Available since

June 30, 2015

Availability

Academic Institutions and Nonprofits only
pET-6xHis-GFP(-30)

Plasmid

#199167

Purpose

used to express a supercharged GFP variant in E. coli

Depositor

Allie Obermeyer

Publication

Yeong et al ACS Cent Sci. 2020 Dec 23;6(12):2301-231

Insert

GFP(-30)

Tags

MGHHHHHHGGA

Mutation

T19D, K36E, N49E, K51E, K62E, R83D, K89D, N159D, …

Promoter

T7

Available since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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