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pBSFI-myr-akt-K179M

Plasmid

#49188

Purpose

cDNA of Akt1 with myristyolation signal and point mutation from K to M at position 179 in cloning vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Mutation

K179M

Promoter

T7

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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3580 pcDNA3 Bad S112A S136A

Plasmid

#8799

Depositor

Stanley Korsmeyer

Article

Harada et al Proc Natl Acad Sci U S A 2001 Aug 14;98(

Insert

Bad (Bad Mouse)

Expression

Mammalian

Mutation

S112A, S136A

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
mSecPrP-pCB6+

Plasmid

#13917

Depositor

Susan Lindquist

Article

Ma et al Proc Natl Acad Sci U S A 2001 Dec 18;98(

Insert

PrP (Prnp Mouse)

Expression

Mammalian

Mutation

3F4 epitag

Available Since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
pcihygroflagsynhDAT

Plasmid

#19990

Depositor

Jonathan Javitch

Article

Saunders et al Proc Natl Acad Sci U S A. 2000 Jun 6. 97

Insert

Dopamine transporter (SLC6A3 Human)

Tags

flag

Expression

Mammalian

Available Since

Sept. 22, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCV-hygro-PAX9

Plasmid

#75083

Purpose

[Edit] retroviral vector holding human PAX9 cDNA

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

PAX9 (PAX9 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Mutation

full length, wild type, without native 3' UTR

Available Since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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pKV015

Plasmid

#64766

Purpose

Recombination-Induced Tag Exchange (RITE) Cassette, HA-GFP to a T7-RFP tag, invariable V5 tag, Hygro selection

Depositor

Fred van Leeuwen

Article

Verzijlbergen et al Proc Natl Acad Sci U S A. 2010 Jan 5;107

Insert

RITE cassette

Use

Rite cassette

Promoter

none

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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GB1-PRD(800-868)

Plasmid

#141345

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(800-868).

Depositor

Lalit Deshmukh

Article

Elias et al Proc Natl Acad Sci U S A. 2020 Sep 11. p

Insert

GB1His6-TEV-ALIX(800-868)

Tags

GB1-His6-TEV

Expression

Bacterial

Available Since

Sept. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pRK010

Plasmid

#69874

Purpose

expression of Channelrhodopsin-2 XXL (extra high expression and long open state, D156C) mutant

Depositor

Robert Kittel, Georg Nagel

Article

Dawydow et al Proc Natl Acad Sci U S A. 2014 Sep 23;11

Insert

Channelrhodopsin-2

Use

Unspecified

Mutation

D156C (XXL)

Promoter

Drosophila Hsp70

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChrimsonR-LSSmOrange-NRN

Plasmid

#149693

Purpose

For packaging and expression of ChrimsonR-LSSmOrange featuring a neuritin (NRN) 3' UTR in neurons via AAV

Depositor

Robert Malenka

Article

Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

Insert

ChrimsonR

Use

AAV

Tags

LSSmOrange

Expression

Mammalian

Promoter

hSyn

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Myc-eIF3f

Plasmid

#35975

Depositor

Susana Valente

Article

Valente et al Proc Natl Acad Sci U S A. 2009 Mar 17;10

Insert

eukaryotic translation initiation factor 3, subunit F (EIF3F Human)

Tags

Myc

Expression

Mammalian

Available Since

April 9, 2012

Availability

Academic Institutions and Nonprofits only

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WNK2 K207A

Plasmid

#24570

Depositor

Joseph Costello

Article

Hong et al Proc Natl Acad Sci U S A. 2007 Jun 26. 1

Insert

WNK2` (WNK2 Human)

Tags

His6 and V5

Expression

Mammalian

Mutation

K207A

Available Since

June 11, 2010

Availability

Academic Institutions and Nonprofits only
p6643 MSCV-P C-FlagHA 31E7-Kozak

Plasmid

#35021

Depositor

Peter Howley

Article

White et al Proc Natl Acad Sci U S A. 2012 Jan 9.

Insert

HPV31 E7

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Promoter

MSCV LTR

Available Since

March 6, 2012

Availability

Academic Institutions and Nonprofits only
pEGFPC1-Ift52

Plasmid

#128875

Purpose

For mammalian expression of mouse Ift52

Depositor

Aimin Liu

Article

Ye et al Proc Natl Acad Sci U S A. 2014 Feb 11;11

Insert

Ift52 (Ift52 Mouse)

Tags

EGFP

Expression

Mammalian

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_vinculin

Plasmid

#65691

Purpose

Expresses Vinculin-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

vinculin (Vcl Mouse)

Use

Luciferase

Tags

CNL

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2016

Availability

Academic Institutions and Nonprofits only
pMBPCas2/3

Plasmid

#89238

Purpose

Plasmid contains the Cas2/3 gene from Pseudomonas aeruginosa PA14, with N-terminal Strep II tag, TEV protease cleavage site, and MBP fusion

Depositor

Blake Wiedenheft

Article

Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

Insert

Cas2/3

Tags

Strep II-MBP

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Chronos-mT-Sapphire

Plasmid

#149690

Purpose

For packaging and expression of Chronos-mT-Sapphire in neurons via AAV

Depositor

Robert Malenka

Article

Christoffel et al Proc Natl Acad Sci U S A. 2021 Jun 15;11

Insert

Chronos

Use

AAV

Tags

mT-Sapphire

Expression

Mammalian

Promoter

hSyn

Available Since

May 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAH36 (Pmyo-2::cGAL-C::let-858 3'UTR)

Plasmid

#107135

Purpose

cGAL-C driver under the control of C. elegans myo-2 promoter

Depositor

Paul Sternberg

Article

Wang et al Proc Natl Acad Sci U S A. 2018 Mar 26. p

Insert

NLS::gp41-1-C-intein::cGAL(AD)

Expression

Worm

Promoter

C. elegans myo-2 promoter

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pUBQ10::ZF108_3xFLAG_YPet

Plasmid

#106441

Purpose

pUBQ10 driven Zinc Finger YPet fusion that targets the FWA promoter in arabidopsis

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

ZF108_3xFLAG_YPet

Use

Synthetic Biology

Expression

Plant

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pBSFI-myr-akt-S473A

Plasmid

#49190

Purpose

cDNA of Akt1 with myristyolation signal and point mutation from S to A at position 473 in cloning vector

Depositor

Peter Vogt

Article

Aoki et al Proc Natl Acad Sci U S A. 1998 Dec 8;95(

Insert

Akt1 (Akt1 Mouse)

Use

Adaptor plasmid

Tags

HA tag and myristolation signal

Mutation

S473A

Promoter

T7

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only
pPRIME-CMV-Neo-FF3

Plasmid

#11665

Purpose

3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of Neomycin and miR30-based shRNA targeting firefly luciferase

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Insert

FF3

Use

Lentiviral

Tags

Neo

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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