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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 7,627 results for: Trac

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Showing: 5921 - 5940 of 7627 results

  6. TAAR8-DuET

    Plasmid
    #213383
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    TAAR8 (TAAR8 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213383-plasmid-map-sequence-id-430738

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  7. TAAR9-DuET

    Plasmid
    #213384
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    TAAR9 (TAAR9 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213384-plasmid-map-sequence-id-430739

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  16. pCSJ982

    Plasmid
    #176622
    Purpose
    Express yeast Ub-MPGLW-Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.
    Depositor
    Alexander Varshavsky
    Article
    Dong et al Proc Natl Acad Sci U S A. 2020 Jun 23;11
    Insert
    Ub-MPGLW-Fbp1
    Tags
    Ubiquitin N-terminal fusion and triple flag tag C…
    Expression
    Yeast
    Mutation
    Changed first 4 N-terminal amino acid residues of…
    Promoter
    TDH3tc3
    Available Since
    March 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    176622-plasmid-map-sequence-id-356527

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  18. F9F17F-GFP

    Plasmid
    #163679
    Purpose
    Expresses a TFR mutant with truncated cytosolic tail and TMD in mammalian cells
    Depositor
    Lei Lu
    Article
    Sun et al J Cell Sci. 2021 Oct 15;134(20). pii: 27
    Insert
    transferrin receptor mutant (TFRC Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    This chimera contains 9 amino acids from the cyto…
    Available Since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    163679-plasmid-map-sequence-id-322819

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  19. pSMT3-FBF2 SS/R

    Plasmid
    #166976
    Purpose
    Expresses FBF-2 with SS/R mutations in repeat 5 in E. coli for purification and crystallization studies
    Depositor
    Traci Hall
    Article
    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1
    Insert
    FBF-2 (fbf-2 Nematode)
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    FBF-2 PUM RNA binding domain; amino acids 164-575…
    Promoter
    T7
    Available Since
    April 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166976-plasmid-map-sequence-id-331635

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  20. pSMT3-FBF2 AQ/Y

    Plasmid
    #166975
    Purpose
    Expresses FBF-2 with AQ/Y mutations in repeat 5 in E. coli for purification and crystallization studies
    Depositor
    Traci Hall
    Article
    Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1
    Insert
    FBF-2 (fbf-2 Nematode)
    Tags
    His-SUMO
    Expression
    Bacterial
    Mutation
    FBF-2 PUM RNA binding domain; amino acids 164-575…
    Promoter
    T7
    Available Since
    April 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    166975-plasmid-map-sequence-id-331630

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Showing: 5921 - 5940 of 7627 results