We narrowed to 14,273 results for: cas9

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pDIV272

Plasmid

#226183

Purpose

Expresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette.

Depositor

Uffe Mortensen

Article

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

tRNA-sgRNA-tRNA

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

TEF1p (Komagataella phaffii)

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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EC64434

Plasmid

#209518

Purpose

Cloning in complete guide expression cassettes, with Cas9 & Cas12a(D156R)

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-dSa-VPR mini.-1X snRP1

Plasmid

#99687

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains with 17 nt snRP1 poly adenylation signal

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VPR mini

Mutation

dead Cas9

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pYZ164

Plasmid

#98408

Purpose

Cas9-gRNA his3 plasmid for his3 deletion in S. pombe

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Insert

gRNA targeting Sp.his3 (his3 Fission Yeast)

Use

CRISPR

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pSH624-ssr

Plasmid

#199241

Purpose

Expresses the gene codying for the Ssr recombinase from Pseudomonas putida DOT-T1E

Depositor

Susanne Häußler

Article

Pankratz et al Nat Protoc. 2023 Oct 5. doi: 10.1038/s41

Insert

single-stranded DNA recombineering

Expression

Bacterial

Promoter

lacIq-Ptrc

Available Since

Oct. 10, 2023

Availability

Academic Institutions and Nonprofits only

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modRNAc0-ABE8e-P2A-GFP

Plasmid

#178177

Purpose

IVT template plasmid for making ABE8e-P2A-GFP modRNA

Depositor

Xiaojun Lian

Article

Haideri et al Cell Rep Methods. 2022 Sep 7;2(9):100290

Insert

ABE8e

Use

Ivt template plasmid

Tags

P2A-GFP

Promoter

T7 promoter

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pSF280

Plasmid

#167675

Purpose

Intermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMH4

Plasmid

#52529

Purpose

serves as PCR-template to generate HR donors for C-terminal 2xFLAG-tag

Depositor

Klaus Foerstemann

Article

Bottcher et al Nucleic Acids Res. 2014 Apr 19.

Insert

2x FLAG-tag

Use

Blunt cloning kit

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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pYTK-DN4

Plasmid

#180285

Purpose

KanR multi-gene yeast expression vector (GFP-dropout)

Depositor

Andreas Milias-Argeitis

Article

Novarina et al STAR Protoc. 2022 Jun 10;3(2):101358. do

Insert

ConLS' - GFP - ConRE' - KanR - CEN6/ARS4

Expression

Bacterial and Yeast

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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BCJJ163

Plasmid

#117512

Purpose

AtRPS5a promoter Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:GGAG:pRPS5a:AATG:BsaI

Expression

Plant

Promoter

AtRPS5a

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pDD378

Plasmid

#91827

Purpose

Codon-optimized HaloTag donor for the SapTrap cloning system

Depositor

Bob Goldstein

Article

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

HaloTag-C1

Use

CRISPR

Expression

Worm

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pCL025 AAVS1-9xTetO-RSV-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

Plasmid

#188743

Purpose

Donor vector for integrating RSV promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Surface marker and mCitrine reporter

Use

Synthetic Biology

Expression

Mammalian

Promoter

RSV

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCL026 AAVS1-9xTetO-hPGK-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

Plasmid

#188744

Purpose

Donor vector for integrating PGK promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Surface marker and mCitrine reporter

Use

Synthetic Biology

Expression

Mammalian

Promoter

PGK

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCL051 AAVS1-9xTetO-nTATAchr21-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

Plasmid

#188745

Purpose

Donor vector for integrating nonTATAchr21 promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Surface marker and mCitrine reporter

Use

Synthetic Biology

Expression

Mammalian

Promoter

nonTATAchr21

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pCL053 AAVS1-9xTetO-nTATAchrX-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRb-T2A-Citrine-PolyA

Plasmid

#188746

Purpose

Donor vector for integrating nonTATAchrX promoter-driven dual surface marker/mCitrine fluorescent reporter at AAVS1 locus

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Insert

Surface marker and mCitrine reporter

Use

Synthetic Biology

Expression

Mammalian

Promoter

nonTATAchrX

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-module1

Plasmid

#222838

Purpose

Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 1)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-module2

Plasmid

#222839

Purpose

Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 2)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-module3

Plasmid

#222840

Purpose

Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 3)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-module4

Plasmid

#222841

Purpose

Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 4)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-module5

Plasmid

#222842

Purpose

Subcloning for Golden Gate assembly of CRISPR/Cas9 vectors (module 5)

Depositor

Hidenori Takeuchi

Type

Empty backbone

Use

CRISPR

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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