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We narrowed to 10,081 results for: crispr plasmids

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Showing: 5921 - 5940 of 10081 results

  1. AICSDP-80:UBTF-HaloTag

    Plasmid
    #133963
    Purpose
    Homology arms and HaloTag-linker sequence for N-terminus tagging of human UBTF
    Depositor
    Allen Institute for Cell Science
    Insert
    UBTF Homology Arms with HaloTag-linker (UBTF Human)
    Use
    CRISPR
    Tags
    HaloTag-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Nov. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    133963-plasmid-map-sequence-id-264347

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  2. AICSDP-116:PCNA-mEGFP

    Plasmid
    #170818
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human PCNA
    Depositor
    Allen Institute for Cell Science
    Insert
    PCNA Homology Arms with mEGFP-linker (PCNA Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    June 11, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    170818-plasmid-map-sequence-id-334731

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  3. AICSDP-079:DCP1A-mEGFP

    Plasmid
    #193922
    Purpose
    Homology arms and mEGFP-SRA for N-terminus tagging of human DCP1A
    Depositor
    Allen Institute for Cell Science
    Insert
    DCP1A Homology Arms with mEGFP-SRA (DCP1A Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Dec. 22, 2022
    Availability
    Industry, Academic Institutions, and Nonprofits
    193922-plasmid-map-sequence-id-380539

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  4. AICSDP-72:TFAM-mEGFP

    Plasmid
    #159745
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TFAM
    Depositor
    Allen Institute for Cell Science
    Insert
    TFAM Homology Arms with linker-mEGFP (TFAM Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Oct. 13, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    159745-plasmid-map-sequence-id-314375

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  5. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

    Plasmid
    #174137
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-mScarlet
    Expression
    Mammalian
    Mutation
    SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…
    Promoter
    CMV and T7
    Available Since
    Sept. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    174137-plasmid-map-sequence-id-341916

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  9. AICSDP-167:CDH5-mEGFP

    Plasmid
    #217425
    Purpose
    Homology arms and mEGFP sequence for C-terminus tagging of human cadherin 5
    Depositor
    Allen Institute for Cell Science
    Insert
    CDH5 Homology Arms with mEGFP (CDH5 Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP
    Mutation
    homology arms contain point mutations to disrupt …
    Available Since
    Nov. 25, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    217425-plasmid-map-sequence-id-454212

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  10. pAN007

    Plasmid
    #220048
    Purpose
    Reporter plasmid that encodes for the GFP fused to an N-terminal flag-HA epitope. A stop codon is inserted between the epitope tag and the gfp sequence to terminate GFP translation.
    Depositor
    Blake Wiedenheft
    Article
    Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1
    Insert
    flag-HA-stop-GFP
    Expression
    Mammalian
    Available Since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220048-plasmid-map-sequence-id-434404

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  11. pAN013

    Plasmid
    #220050
    Purpose
    Reporter plasmid that expresses GFP and firefly luciferase linked with a viral 2A peptide. A stop codon is inserted at the 3'-end of the gfp gene, which permits GFP, but not luciferase expression.
    Depositor
    Blake Wiedenheft
    Article
    Nemudraia et al Science. 2024 Apr 25:eadk5518. doi: 10.1
    Insert
    GFP-stop-fLuc
    Expression
    Mammalian
    Available Since
    May 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    220050-plasmid-map-sequence-id-434403

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  13. pINSPECT:IL2_SigP:NLuc

    Plasmid
    #197266
    Purpose
    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human IL2 locus (exon 3). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Insert
    Interleukin-2 homology arms
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Expression
    Mammalian
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197266-plasmid-map-sequence-id-389716

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  14. pINSPECT:NEAT1-v2_SigP:NLuc

    Plasmid
    #197268
    Purpose
    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (long isoform only). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Insert
    NEAT1-v2 homology arms
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Expression
    Mammalian
    Available Since
    April 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    197268-plasmid-map-sequence-id-389661

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  15. pINSPECT:NEAT1-total_SigP:NLuc

    Plasmid
    #197267
    Purpose
    Donor plasmid for CRISPR/Cas9-mediated insertion of INSPECT into human NEAT1 locus (both isoforms). Encodes for intronic IRES-driven SigP:NLuc. Plasmid contains FRT-site flanked PuroR-TK cassette.
    Depositor
    Gil Westmeyer
    Article
    Truong et al Nat Cell Biol. 2022 Nov;24(11):1666-1676
    Insert
    NEAT1-total homology arms
    Use
    CRISPR, Luciferase, Synthetic Biology, and Unspec…
    Expression
    Mammalian
    Available Since
    July 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    197267-plasmid-map-sequence-id-400583

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Showing: 5921 - 5940 of 10081 results