We narrowed to 6,854 results for: itch
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Plasmid#60033PurposeExpresses a fusion of full length CRY2 (E490G) with mCherryDepositorInsertCRY2 E490G (deltaNLS) (CRY2 Mustard Weed)
ExpressionMammalianMutationE490G, NLS mutatedPromoterCMVAvailable SinceOct. 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDBTrp-LexABD-CRY2FL
Plasmid#78210PurposeUse with Addgene 40615 for blue light transcription control in yeastDepositorInsertLexABD-CRY2 (CRY2 Mustard Weed)
TagslexA binding domainExpressionYeastMutationcontains endogenous CRY2 NLSPromoterADH1Available SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDB22
Plasmid#61342PurposeMammalian expression of mCherry fused to LINuS, a light-inducible nuclear localization signal (cMycP1A NLS/PKIt NES)DepositorInsertmCherry
TagsGS linker-AsLov2-cMyc1A NLS and PKIt NESExpressionMammalianPromoterCMVAvailable SinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
B2094_CRY2olig-miRFP
Plasmid#215114PurposeExpresses CRY2olig (E490G CRY2 1-498) fused to miRFP in mammalian cellsDepositorInsertCRY2olig-miRFP (CRY2 Mustard Weed)
TagsmiRFPExpressionMammalianMutationE490GPromoterCMVAvailable SinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCherry-OptoPB
Plasmid#113935PurposeExpress a LOV2 fusion protein to enable light-dependent cytosol-to-PM translocation and dissect protein-phosphoinositide interactionsDepositorInsertOptoPB
TagsmCherryExpressionBacterial and MammalianPromoterCMVAvailable SinceJuly 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-HA-β2AR-NNES-NanoLuc-15aa linker-AsLOV2(V416L)
Plasmid#125224PurposeExpresses β2AR-NanoLuc-AsLOV2(V416L) in mammalian cellsDepositorInsertHA-β2AR-NNES-NanoLuc-15aa linker-AsLOV2(V416L)
UseAAVTagsHAExpressionMammalianPromoterCMVAvailable SinceMay 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM0646
Plasmid#129007PurposeIntegration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2 with loxable KIURA3 markerDepositorInsertKanR-ColE1 URA 5'-pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZCRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-loxP-KIURA3-loxP-URA3 3'
ExpressionYeastAvailable SinceOct. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CRY2-mCh-superPLDx10
Plasmid#188986PurposeA lentiviral plasmid encoding CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type)DepositorInsertPLD
TagsCRY2 and mCherryExpressionMammalianMutationF163L, K249R, G328S, G381V, G429D, D480GPromoterCMVAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pVG122
Plasmid#211765PurposeExpresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.DepositorInsertstrongLOV
UseFor single-copy integration (if cut with pmei)TagsSV40 NLS and VP16ExpressionYeastMutationcompared to EL222, strongLOV contains Glu84Asp mu…Available SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTMEM-PhoCl-Insert
Plasmid#219674PurposeCaging at cytosolic N-ter of POI: TMEM-PhoCl-rtta3mScarletDepositorInsertTMEM-PhoCl-rtTA3
TagsPhoClExpressionMammalianPromoterCMVAvailable SinceJuly 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
Opto-casp9_VfAU1-LOV_casp9
Plasmid#131220PurposeExpresses Opto-casp9_VfAU1-LOV_casp9 (light-activated caspase9)DepositorInsertOpto-casp9 (VfAU1-LOV_casp9)
ExpressionMammalianPromoterCMVAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CRY2-mCh-superPLDx30
Plasmid#188988PurposeA lentiviral plasmid encoding CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type)DepositorInsertPLD
TagsCRY2 and mCherryExpressionMammalianMutationK109R, P245A, G328S, G381V, G429DAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-LexA-WW-CIB1-biLINuS
Plasmid#171757PurposeLentiviral vector for constitutive expression of fusion protein LexA-WW-CIB1-biLINuSDepositorInsertSynthetic gene containing LexA-WW-CIB1-biLINuS
UseLentiviralExpressionMammalianPromoterPGKAvailable SinceSept. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDN42
Plasmid#61345PurposemCherry fused to LINuS, a light-inducible nuclear localization signal (biNLS10/PKIt NES)DepositorInsertmCherry
TagsGS linker-AsLov2-biNLS10 and PKIt NESExpressionMammalianPromoterCMVAvailable SinceMay 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
CLPIT Cry2PHR-mCh-RhoA
Plasmid#42959DepositorInsertCry2PHR-mCherry-RhoA (RHOA Human, Mustard Weed)
UseRetroviralExpressionMammalianPromoterTet-Off promoterAvailable SinceApril 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
rAAV-syn::FLEX-rev::PSAML141F:GlyR-IRES-GFP
Plasmid#32479PurposeCre-dependent expression of PSAM-GlyR (L141F) neuronal inhibitor, with IRES-EGFP markerDepositorInsertPSAML141F-GlyR-IRES-GFP
UseAAV and Cre/Lox; Adeno-associated virus, flex swi…TagsIRES-EGFPPromoterSynapsinAvailable SinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMM0638
Plasmid#129005PurposeIntegration vector for optogenetic machinery (pSTRONG-VP16-CIB1/pMEDIUM-ZCRY2PHR) and pZF-mRUBY2DepositorInsert5' URA3 -pTEF1-SV40NLS-VP16-CIB1-tENO1-pRPL18B-SV40NLS-ZiF268DBD-CRY2PHR-tSSA1-pZF(BS)-mRUBY2-tADH1-URA3 URA3 3'- KanR-ColE1
ExpressionYeastAvailable SinceOct. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CibN-dCas9
Plasmid#60551PurposeExpresses CibN-dCas9 in mammalian cellsDepositorInsertCibN-dCas9
UseCRISPRTagsFLAG and SV40 NLSExpressionMammalianMutationinactivating Cas9 mutations D10A and H840APromoterCMVAvailable SinceFeb. 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
B2375_CRY2olig-mEGFP
Plasmid#215109PurposeExpresses CRY2olig (E490G CRY2 1-498) fused to monomeric EGFP in mammalian cellsDepositorInsertCRY2olig-mEGFP (CRY2 Mustard Weed)
TagsmEGFPExpressionMammalianMutationE490G, 1-498PromoterCMVAvailable SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
optoPKAW196R/Y204A
Plasmid#104546PurposeExpresses Cry2-mCherry-PKA W196R/Y204A in mammalian cells.DepositorInsertCry2-mCherry-PKA W196R/Y204A
TagsmCherryExpressionMammalianMutationPKA W196R and Y204APromoterCMVAvailable SinceMarch 12, 2018AvailabilityAcademic Institutions and Nonprofits only