We narrowed to 81,336 results for: ELL

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FASTHDR-Cterm-HaloTag-Puromycin

Plasmid

#167208

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with HaloTag and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

HaloTag

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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Yiplac211 proGAL1-TsCC(A)-RGG-GFP-RGG-RGG

Plasmid

#176395

Purpose

Expresses GFP tagged triple RGG scaffold with N-terminal TsCC(A) coiled coil. Expression induced in media containing galactose.

Depositor

Matthew Good

Article

Garabedian et al Nat Chem Biol. 2021 Sep;17(9):998-1007.

Insert

GAL1 promoter, TsCC(A)-RGG-GFP-RGG-RGG

Expression

Yeast

Promoter

GAL1

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-mCD47-T2A-GFP

Plasmid

#205449

Purpose

lentiviral plasmid for expression of mouse CD47

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

mCD47 (Cd47 Mouse)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pMYH9-WT-V5

Plasmid

#183512

Purpose

Gateway expression vector encoding wild type MYH9 cDNA with N-terminal V5 tag. For mammalian expression.

Depositor

Emanuele Panza

Article

Panza et al Pathogenetics. 2008 Dec 1;1(1):5. doi: 1

Insert

MYH9 (MYH9 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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pmScarlet_Giantin_C1

Plasmid

#85048

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Giantin (GOLGB1 Human)

Expression

Mammalian

Mutation

aa 3131-3259

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-GFP-FLAG-cGAS E225A-D227A

Plasmid

#86674

Purpose

Expresses catalytically-inactive GFP-cGAS after lentiviral transduction. Can be used to monitor nuclear envelope rupture events.

Depositor

Nicolas Manel

Article

Raab et al Science. 2016 Apr 15;352(6283):359-62. d

Insert

GFP-FLAG-cGAS E225A/D227A

Use

Lentiviral

Available Since

April 18, 2017

Availability

Academic Institutions and Nonprofits only

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APEX2-SOPP3-TM

Plasmid

#229077

Purpose

Expresses APEX2-SOPP3 in the cell surface

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express APEX2-SOPP3 in the cell surface (APEX2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-hCD47-T2A-GFP

Plasmid

#205458

Purpose

lentiviral plasmid for expression of human CD47

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

hCD47 (CD47 Human)

Expression

Mammalian

Promoter

UBC

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pmScarlet-i_Giantin_C1

Plasmid

#85050

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Giantin (GOLGB1 Human)

Expression

Mammalian

Mutation

aa 3131-3259

Promoter

CMV

Available Since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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Cre-p2a-tdTomato

Plasmid

#176837

Purpose

Express Cre in mammalian cells and enable FACS sorting Cre expressing cells by tdTomato expression

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

Insert

Cre-P2A-tdTomato

Expression

Mammalian

Promoter

CAGGS

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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FUW-Sox10

Plasmid

#36978

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

Sox10 (SOX10 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 30, 2012

Availability

Academic Institutions and Nonprofits only

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FASTHDR-Cterm-NanoLuc-Puromycin

Plasmid

#167209

Purpose

Plasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with NanoLuc and selection with Puromycin

Depositor

Oscar Perez

Article

Perez-Leal et al CRISPR J. 2021 Nov 30. doi: 10.1089/cris

Type

Empty backbone

Use

CRISPR

Tags

NanoLuc

Expression

Mammalian

Available Since

Dec. 8, 2021

Availability

Academic Institutions and Nonprofits only

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A2_uSFFV

Plasmid

#153415

Purpose

Expresses HLA-A*02:01 MHCI gene by the UCOE-SFFV promoter

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

A*02:01-P2A-human beta2 microglobulin (B2M Human)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pWPI-N1ICD

Plasmid

#185525

Purpose

Constitutive overexpression of human NOTCH1 intracellular domain

Depositor

Sean Palecek, Eric Shusta

Article

Gastfriend et al Sci Adv. 2024 Feb 2;10(5):eadi1737. doi:

Insert

NOTCH1 intracellular domain (NOTCH1 Human)

Use

Lentiviral

Promoter

EF-1a

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector

Plasmid

#159281

Purpose

A multicistronic vector with both CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA)

Depositor

Rudolf Jaenisch

Article

Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

Insert

AsCpf1-HA-2A-GFP

Use

Multicistronic vector with both caggs promoter-dr…

Tags

3X HA

Expression

Mammalian

Promoter

U6

Available Since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-NOTUM-EF1a-TagRFP-2A-tet3G

Plasmid

#204188

Purpose

Doxycycline inducible lentiviral vector for human NOTUM overexpression

Depositor

Emma Rawlins

Article

Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

Insert

NOTUM (NOTUM Human)

Use

Lentiviral

Expression

Mammalian

Promoter

TetON

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Puro-TK-NLS-mTurq2-PCNA

Plasmid

#118617

Purpose

Lentiviral expression of mTurq2 tagged PCNA

Depositor

Jean Cook

Article

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

TK-NLS-mTurq2-PCNA (PCNA Human)

Use

Lentiviral

Tags

mTurq2

Expression

Mammalian

Promoter

hPGK

Available Since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pSB-XPRESSO-hSpCas9-EGFP

Plasmid

#237298

Purpose

Sleeping Beauty XPRESSO vector for Cas9-EGFP expression

Depositor

Lior Gepstein

Article

Wexler et al Stem Cell Reports. 2025 Aug 19:102603. d

Insert

spCas9 (cas9 Stretococcus pyogenes)

Use

CRISPR

Tags

eGFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pcS-RDG-C1C2

Plasmid

#200163

Purpose

This plasmid encodes non-binder control monobody (RDG) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.

Depositor

Masako Harada

Article

Komuro et al Bioengineering (Basel). 2022 Jan 29;9(2)

Inserts

RDG-C1C2
C1C2 domain of lactadherin

Tags

HA and HIS

Expression

Mammalian

Mutation

sequence adopted from doi: 10.1371/journal.pone.0…

Available Since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-JNKKTRrsGreenF-E-2A-ERKKTRrsFastLime-IRES-PKAKTRClover-2A-P38KTRDronpa

Plasmid

#205766

Purpose

Expression of JNK KTR rsGreenF-E, ERK KTR rsFastLime, PKA KTR Clover, and P38 KTR Dronpa in mamalian cells

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

JNK KTR rsGreenF-E-2A-ERK KTR rsFastLime-IRES-PKA KTR Clover-2A-P38 KTR Dronpa

Use

AAV

Expression

Mammalian

Mutation

wt

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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