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343 pTol2-fli1epDest

Plasmid

#73486

Purpose

Tol2 vector with chimeric endothelial enhancer/promoter upstream of attR1/R2 cassette and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

fli1ep

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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mRuby-BaxAlpha-C-15

Plasmid

#55847

Purpose

Localization: , Excitation: 558, Emission: 605

Depositor

Michael Davidson

Insert

BaxAlpha

Tags

mRuby

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits
T7 promoter + rpsL reporter plasmid

Plasmid

#156455

Purpose

A mutational reporter plasmid that can gain Streptomycin and/or Tetracycline resistance upon mutation. Contains T7 promoter.

Depositor

Matthew D. Shoulders

Article

Moore et al J Am Chem Soc. 2018 Sep 19;140(37):11560

Inserts

rpsL
TcR

Expression

Bacterial

Mutation

ATG start codon mutated to ACG

Promoter

tac and tet

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mEos4b (mEosFP4b-V69T)

Plasmid

#99226

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos4b (mEos4b-V69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos4b

Expression

Bacterial

Mutation

V69T for primed conversion applications

Promoter

T7

Available Since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pNP2157

Plasmid

#222732

Purpose

pNP1950 - J23119-IS621 bRNA TBL-WT, DBL-WT, HDV; T7-IS621 Recombinase

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Insert

IS621 Helper (WT)

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pNP1830

Plasmid

#222728

Purpose

pNP561 - T5 Target with T7 Inducible IS621 Recombinase

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Insert

IS621 Recombinase

Expression

Bacterial

Available Since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-SYN-sDarken

Plasmid

#184796

Purpose

Serotonin Sensor sDarken under the control of a hSyn Promoter.

Depositor

Olivia Masseck

Article

Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

Insert

sDarken

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pMAK463

Plasmid

#140701

Purpose

NBαMs-IgK

Depositor

Takayuki Iwaki

Article

Iwaki et al Protein Expr Purif. 2020 Feb 13;170:1056

Insert

NBαMs-IgK

Expression

Bacterial

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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pNB2289

Plasmid

#113396

Purpose

Galactose-inducible red fluorescent protein (RFP) codon optimized for S. cerevisiae

Depositor

Suzanne Sandmeyer

Insert

Red fluorescent protein, codon optimized for S. cerevisiae

Expression

Yeast

Available Since

Aug. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pBS-IT10

Plasmid

#66871

Purpose

Inntag 10: a small self-structured epitope aimed at innocuous protein tagging

Depositor

Marti Aldea

Article

Georgieva et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

Insert

Inntag 10

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only
pUPD2 RDF ( GB1498)

Plasmid

#160579

Purpose

Streptomyces phage PhiC31-encoded recombination directionality factor (RDF) (PhiC31 gp3)

Depositor

Diego Orzaez

Article

Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

Insert

RDF

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAG330 td-FAST

Plasmid

#130810

Purpose

Expresses td-FAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Biochemistry. 2018 Oct 2;57(39):5648-565

Insert

td-FAST

Tags

myc-tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pJMP3654

Plasmid

#222349

Purpose

Expression vector with GFP expressed under PabstBR promoter, kanR marker, and replicates in A. baumannii and E. coli.

Depositor

Jason Peters

Article

Bacon et al Microbiol Spectr. 2024 Nov 5;12(11):e013

Insert

sfGFP

Expression

Bacterial

Promoter

PabstBR

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pNIC-HIS6-MBP-TEV-GG

Plasmid

#215800

Purpose

Cloning of target gene with N terminal His6 MBP tag and TEV Cleavage

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pSF11-wElectra1

Plasmid

#184932

Purpose

Codon optimized blue fluorescent protein Electra1 in C.elegans expresison plasmid

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

Electra1

Expression

Worm

Promoter

tag-168

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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mUAVbla:tetR 5′

Plasmid

#149379

Purpose

mUAVbla containing a tetracycline resistance (tetA) cassette for building Gene Doctoring donor plasmids from pDOC-GG, using BsaI Golden Gate assembly with GGAG and CGCT overhangs

Depositor

Mark Pallen

Article

Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi

Insert

tetA

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCO601

Plasmid

#139733

Purpose

Module plasmid, individual component of Fungal Bioluminescent Pathway, Hisps

Depositor

Daniel Voytas

Article

Khakhar et al Elife. 2020 Mar 25;9. pii: 52786. doi: 1

Insert

pMOD_C'_p35S:NnHisps:t35S

Use

Luciferase

Expression

Plant

Promoter

35S

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_WTDHFR_WTTS

Plasmid

#91226

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pAJM.721

Plasmid

#108522

Purpose

PSalTTC-YFP Reporter. Sal sensor for use in Marionette strain.

Depositor

Christopher Voigt

Article

Meyer et al Nat Chem Biol. 2018 Nov 26. pii: 10.1038

Insert

PSalTTC-YFP

Expression

Bacterial

Available Since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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CytERM-dTomato

Plasmid

#98834

Purpose

In vivo visualization of the ER (can be used for colocalization studies and the OSER assay)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

CytERM

Tags

dTomato

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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