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Plasmid#78462PurposeInducible expression in E. coliDepositorInsertPolymerase theta (POLQ Human)
UseTagsHis and SUMO3ExpressionBacterialMutationdelta 1-1791; Q2513R (please see depositor commen…PromoterT7Available sinceJuly 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET11a-SUMO1
Plasmid#53138PurposeE.coli expression of untagged mature human SUMO1 (amino acids 1-97)DepositorInsertSUMO1 (SUMO1 Human)
UseTagsExpressionBacterialMutationmature form (amino acids 1-97)PromoterT7Available sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET11a-SUMO3
Plasmid#53143PurposeE.coli expression of untagged mature human SUMO3 (amino acids 1-93)DepositorInsertSUMO3 (SUMO2 Human)
UseTagsExpressionBacterialMutationmature form (amino acids 1-93)PromoterT7Available sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
hSUMO1-HIS
Plasmid#118361PurposeThis plasmid expresses the full length human SUMO1.DepositorInserthSUMO1 (SUMO1 Human)
UseTagsHISExpressionMammalianMutationPlease see Depositor CommentsPromoterAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11a-SUMO2
Plasmid#53142PurposeE.coli expression of untagged mature human SUMO2 (amino acids 1-92)DepositorInsertSUMO2 (SUMO3 Human)
UseTagsExpressionBacterialMutationmature form (amino acids 1-92)PromoterT7Available sinceJune 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO-TtAgo
Plasmid#210191PurposeBacterial expression of the His- and SUMO-tagged Argonaute protein of Thermus thermophilusDepositorInsertTtAgo (TT_P0026 Thermus thermophilus)
UseTags6x His tag and SUMO tagExpressionBacterialMutationPromoterT7Available sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEYFP SUMO-1
Plasmid#13380DepositorInsertSUMO-1 (SUMO1 Human)
UseTagsEYFPExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2007AvailabilityAcademic Institutions and Nonprofits only -
pESUMO-hRORg1
Plasmid#170330PurposeExpresses RORg isoform 1 with N-terminal HisSUMO tag in bacteriaDepositorInsertRORG isoform 1 (RORC Human)
UseTagsHisSUMOExpressionBacterialMutationPromoterT7Available sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-(SUMO)3
Plasmid#127093PurposeBacterial expression plasmid for eGFP-(SUMO)3 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP - 3 repeats of human SUMO3 each separated by (GGS)4 (SUMO3 Human)
UseTagsEGFPExpressionBacterialMutationPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
6598-HisSUMO1GG
Plasmid#13376DepositorInsertSUMO1 (SUMO1 Human)
UseT7 in vitro translationTagsHisExpressionMammalianMutationmature form ending with GGPromoterAvailable sinceDec. 8, 2006AvailabilityAcademic Institutions and Nonprofits only -
PE-SUMO CRYAB
Plasmid#217670PurposeFor expression of SUMO tagged human alpha B crystallinDepositorInsertCRYAB (CRYAB Human)
UseTags6XHis and Smt3ExpressionBacterialMutationPromoterAvailable sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
PE-SUMO CRYBB1
Plasmid#217675PurposeFor expression of SUMO tagged human beta B1 crystallinDepositorInsertCRYBB1 (CRYBB1 Human)
UseTags6XHis and Smt3ExpressionBacterialMutationPromoterT7Available sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
SUMO_TwinStrep_Cas7-11_crRNA
Plasmid#197612PurposeFor protein purification of Cas7-11DepositorInsertSUMO_TwinStrep_Cas7-11 with crRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
hSUMO1 GFP
Plasmid#118360PurposeThis plasmid expresses the full length human SUMO1.DepositorInserthSUMO1 (SUMO1 Human)
UseTagsEGFPExpressionMammalianMutationWTPromoterAvailable sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pE-SUMO CRYAA
Plasmid#80756PurposeExpression of human alpha A-crystallin in E. coliDepositorInsertCRYAA (CRYAA Human)
UseTags6xHIS and Smt3 SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET28-MKH8SUMO
Plasmid#79526PurposeBacterial expression plasmid used for T7 promoter driven expression of recombinant proteins with N-terminal 8X His, thrombin cleavage site, SUMO, and TEV cleavage site.DepositorTypeEmpty backboneUseTags8xHis-Thrombin-SUMO-TEV and TEV cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 16, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
(SUMO)10-(SIM)5
Plasmid#126866PurposeBacterial expression plasmid for multivalent scaffold of (SUMO)10-(SIM)5 biomolecular condensates to recruit SIM-containing client proteins.DepositorInsert10 repeats of human SUMO3 and 5 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
UseTagsExpressionBacterialMutationPromotertacAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-(SUMO)1
Plasmid#127089PurposeBacterial expression plasmid for eGFP-(SUMO)1 client to assess SUMO-SIM scaffolds.DepositorInsertEGFP human SUMO3 (SUMO3 Human)
UseTagsEGFPExpressionBacterialMutationPromoterunknownAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSUMO-YHRC
Plasmid#54336PurposeExpression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.DepositorTypeEmpty backboneUseTags6His-SUMO (yeast gene: SMT3)ExpressionBacterialMutationPromoterT7-LacOAvailable sinceAug. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
(SUMO)5-(SIM)10
Plasmid#126923PurposeBacterial expression plasmid for multivalent scaffold of (SUMO)5-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.DepositorInsert5 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
UseTagsExpressionBacterialMutationinternal BamHI site between (SUMO)5 and (SIM)10PromotertacAvailable sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pE-SUMO CRYBB2
Plasmid#80749PurposeExpression of human beta B2-crystallin in E. coliDepositorInsertCRYBB2 (CRYBB2 Human)
UseTags6xHIS and Smt3 SUMOExpressionBacterialMutationPromoterT7Available sinceMarch 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmRFP-SUMO1
Plasmid#13989DepositorInsertSUMO1 (Sumo1 Mouse)
UseTagsmRFPExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2007AvailabilityAcademic Institutions and Nonprofits only -
RFP-(SUMO)3
Plasmid#127150PurposeMammalian expression plasmid for mCherry-(SUMO)3 client to assess SUMO-SIM scaffolds.DepositorInsert3 copies of human SUMO1 separated by (GGS)4 (SUMO3 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
ShadowG-SUMO1
Plasmid#175239PurposeBacterial expression and purification, conjugatable SUMO for SUMOylationDepositorInsertSUMO1 (SUMO1 Human)
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Rhodoplanes-bGSDM
Plasmid#212129PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Rhodoplanes species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertRhodoplanes bGSDM
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Bacteroidetes-bGSDM
Plasmid#212124PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Bacteroidetes (metagenomic) species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertBacteroidetes bGSDM
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Runella-bGSDM
Plasmid#212123PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by Runella zeae. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertRunella bGSDM
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Vitiosangium-bGSDM
Plasmid#212127PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Vitiosangium species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertVitiosangium bGSDM
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Gillisia-bGSDM
Plasmid#212128PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Gillisia species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertGillisia bGSDM
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Bradyrhizobium-bGSDM
Plasmid#212126PurposeFor expression of the bacterial gasdermin (bGSDM) encoded by a Bradyrhizobium species. Protein is expressed with an N-terminal SUMO2 tag.DepositorInsertBradyrhizobium bGSDM
UseTags6xHis-SUMO2ExpressionBacterialMutationN-terminal methionine deleted and replaced by sin…PromoterT7Available sinceJan. 8, 2024AvailabilityAcademic Institutions and Nonprofits only