Addgene

We narrowed to 310 results for: PLL3

Showing: 41 - 60 of 310 results

Results per page:

Sort results by:

pLL3 sgRNA(MS2)-MS2-AID3f

Plasmid

#112129

Purpose

empty sgRNA cloning vector with MS2-AID3f

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

AIDmono 3f mutant (AICDA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

N- mutated and C- terminal truncated, F115Y/C116F…

Promoter

hU6,CBh

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL3.7-K122-TEAD-p-H2B-mCherry

Plasmid

#139648

Purpose

Reporter assay

Depositor

Yutaka Hata

Article

Kawano et al J Biochem. 2015 May 14. pii: mvv051.

Insert

IRES-GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL3.7m-mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1

Plasmid

#83842

Purpose

Fluorescent probe for S/G2 cell cycle transition and G2/M transition

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mTurquoise2-SLBP(18-126)-IRES-H1-mMaroon1 (H1-0, SLBP Human, Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry

Plasmid

#128327

Purpose

Reporter to evaluate YAP1/TEAD-mediated gene transcription

Depositor

Yutaka Hata

Article

Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

Inserts

H2B mCherry
YAP1 (YAP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL3.7m-YFP-SLBP(18-126)-IRES-H1-Skylan62A

Plasmid

#205760

Purpose

Single-color FUCCI4 sensor for S/G2 cell cycle transition and G2/M transition

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

YFP-SLBP(18-126)-IRES-H1-Skylan62A

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL3.7-K122-FH-TAZ-TEAD-p-H2B-mCherry

Plasmid

#139649

Purpose

Reporter assay

Depositor

Yutaka Hata

Article

Kawano et al J Biochem. 2015 May 14. pii: mvv051.

Insert

H2B-mCherry

Use

Lentiviral

Tags

FLAG, His6

Expression

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL3.7m-Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120)

Plasmid

#83841

Purpose

Fluorescent probe for G1/S transition and M/G1 transition

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

Clover-Geminin(1-110)-IRES-mKO2-Cdt(30-120) (GMNN, CDT1 Human, Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL3.7m-rsGreenF-E-Geminin(1-110)-IRES-Dronpa-Cdt(30-120)

Plasmid

#205761

Purpose

Single-color FUCCI4 sensor for G1/S cell cycle transition and M/G1 transition

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsGreenF-E-Geminin(1-110)-IRES-Dronpa-Cdt(30-120)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL3.7 K122 -ires-GFP-Osteocalcin-promoter-H2B mCherry reporter

Plasmid

#68714

Depositor

Yutaka Hata

Insert

H2B mCherry and GFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL3.7m-rsGreenF-E-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-Skylan62A-2A-YFP-SLBP

Plasmid

#205762

Purpose

Single-color FUCCI4 senor for transitions of all four cell cylce phases

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsGFE-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-SK62A-2A-YFP-SLBP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

wt

Promoter

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLL3.7 K122 FH-TAZ-ires-GFP-Osteocalcin-promoter-H2B mCherry reporter

Plasmid

#68713

Depositor

Yutaka Hata

Insert

H2B mCherry and TAZ/WWTR1

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV for TAZ/WWTR1

Available since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only
LV 1-5

Plasmid

#68411

Purpose

Lentiviral backbone for use with GMAP

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Type

Empty backbone

Use

Lentiviral; Gmap

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2268

Plasmid

#175963

Purpose

Genetic complementation plasmid of LetA-[LetB PLL3->6] mutant

Depositor

Damian Ekiert, Gira Bhabha

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Inserts

LetA
LetB

Use

Tags

Expression

Bacterial

Mutation

Δ347-361, replace with 702-716

Promoter

T7

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1995

Plasmid

#139899

Purpose

Expression plasmid for LetB PLL3 deletion (without TM region)

Depositor

Damian Ekiert, Gira Bhabha

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetB

Use

Tags

Expression

Bacterial

Mutation

LetB(43-877; delta347-360, P346G)-6xHis

Promoter

Available since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL1888

Plasmid

#139892

Purpose

Complementation plasmid for LetAB with LetB PLL3 deletion

Depositor

Damian Ekiert, Gira Bhabha

Article

Isom et al Cell. 2020 Apr 30;181(3):653-664.e19. do

Insert

LetAB

Use

Tags

Expression

Bacterial

Mutation

LetA-LetB(delta347-360, P346G)

Promoter

Available since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBEL2265

Plasmid

#175962

Purpose

For expresson of LetA-[LetB PLL3->6] operon from E. coli. N-terminal his tag on LetA, araBAD promoter

Depositor

Damian Ekiert, Gira Bhabha

Article

Vieni et al J Mol Biol. 2022 Jan 22;434(7):167463. d

Inserts

LetA
LetB

Use

Tags

8xHis 2xQH and TEV cleavage site on LetA

Expression

Bacterial

Mutation

Δ347-361, replace with 702-716

Promoter

araBAD

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pLB

Plasmid

#11619

Purpose

pLB is a modification of pLL3.7 (Addgene#11795). Genetic elements known to prevent epigenetic silencing were added.

Depositor

Stephan Kissler

Article

Kissler et al Nat Genet. 2006 Apr . 38(4):479-83.

Type

Empty backbone

Use

Cre/Lox, Lentiviral, and RNAi

Tags

GFP

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

July 19, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pLV-shEsr1

Plasmid

#120720

Purpose

Lentiviral vector that expresses GFP and an shRNA targeting Esr1 (in pLL3.7).

Depositor

Amy Lasek

Article

Satta et al Int J Neuropsychopharmacol. 2018 Apr 1;2

Insert

Esr1 shRNA (Esr1 Mouse)

Use

Lentiviral and RNAi

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Mouse U6

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
hu6-sgCebpd_v2 (opti)

Plasmid

#177250

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses Cebpd gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgCebpd_2nd

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
hU6-sgNT1 (opti)

Plasmid

#177237

Purpose

Same as pLL3.3;U6(BstXI)-XhoI-chimeric RNA;PGK-Cre but XhoI stuffer removed, expresses non-targeting gRNA

Depositor

Monte Winslow

Article

Murray et al Nat Commun. 2022 Feb 28;13(1):1090. doi:

Insert

sgNT1

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge