We narrowed to 31,524 results for: REP

1. RepairTemplate_NIPBL_mEGFP_mAID

   Plasmid

   #217664

   Purpose

   Repair template to endogenously tag NIPBL with mEGFP-mAID Tag

   Depositor

   Daniel Gerlich

   Article

   Batty et al EMBO J. 2023 Aug 15;42(16):e113475. doi:

   Insert

   NIPBL_mEGFP_mAID (NIPBL Human)
   

   Tags

   mAID and meGFP

   Expression

   Bacterial and Mammalian

   Available Since

   July 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. 50 pLIB_STREP

   Plasmid

   #160275

   Purpose

   N-term 3C cleavable 2xStrepII tag for insect cell expression

   Depositor

   John Weir

   Article

   Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

   Type

   Empty backbone

   Tags

   2xStrepII

   Expression

   Insect

   Promoter

   polyhydrin

   Available Since

   Dec. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKERepPar

   Plasmid

   #211827

   Purpose

   Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein and a RP4 partitioning system to increase segregational stability.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pREP

   Plasmid

   #51491

   Purpose

   pMDC32 with Rep/RepA coding sequence from the mild bean yellow dwarf virus

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Insert

   Rep/RepA
   

   Use

   Plant expression t-dna

   Mutation

   none

   Promoter

   2x35S

   Available Since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBz15_Strep_Rubisco

   Plasmid

   #140839

   Purpose

   wild-type Halothiobacillus neapolitanus Form I Rubisco with StrepII tag (CbbL-StrepII, CbbS), pET-14b backbone

   Depositor

   David Savage

   Article

   Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

   Insert

   Form IA Rubisco; CbbL, CbbS
   

   Tags

   Strep II

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pReporter_12

   Plasmid

   #60572

   Purpose

   A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Reporter_12
   

   Expression

   Bacterial

   Promoter

   BBa_J23119

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKERep

   Plasmid

   #211826

   Purpose

   Medium copy number protein expression plasmid, suitable for electroporation in C. necator. Containes eGFP as model protein.

   Depositor

   Anita Emmerstorfer-Augustin

   Article

   Arhar et al Microb Cell Fact. 2024 Jan 3;23(1):9. do

   Insert

   eGFP
   

   Expression

   Bacterial

   Promoter

   T5

   Available Since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRep

   Plasmid

   #199599

   Purpose

   Expresses AAV2 rep gene; accessory plasmid for the pCMV-Cap SSM library.

   Depositor

   George Church

   Article

   Ogden et al Science. 2019 Nov 29;366(6469):1139-1143

   Insert

   AAV2 rep
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. gRNA_tdtomato_reporter1_SP-2x

   Plasmid

   #79370

   Purpose

   Assays activity of transcriptional activators fused to SP Cas9

   Depositor

   George Church

   Article

   Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

   Insert

   tdtomato
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   April 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. WT (REP10)

    Plasmid

    #21368

    Depositor

    Gregory Germino

    Article

    Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 4, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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11. SUMO_TwinStrep_Csx30

    Plasmid

    #197611

    Purpose

    For protein purification of Csx30

    Depositor

    Feng Zhang

    Article

    Strecker et al Science. 2022 Nov 25;378(6622):874-881.

    Insert

    SUMO_TwinStrep_Csx30
    

    Expression

    Bacterial

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pREPK219H

    Plasmid

    #51492

    Purpose

    pREP with a mutation in the ATPase domain (K219H). This is predicted to result in loss of geminivirus replication.

    Depositor

    Daniel Voytas

    Article

    Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

    Insert

    Rep/RepA
    

    Use

    Plant expression t-dna

    Mutation

    changed lysine 219 to histidine

    Promoter

    2x35S

    Available Since

    May 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. 28 pCOLI_S_STREP

    Plasmid

    #160262

    Purpose

    N-term 2xStrepII 3C cleavable Spec resistant bacterial expression vector

    Depositor

    John Weir

    Article

    Altmannova et al Protein Sci. 2020 Sep 21. doi: 10.1002/p

    Type

    Empty backbone

    Tags

    2xStrepII

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pYZ_3WJrep_N20_switch

    Plasmid

    #132722

    Purpose

    T7 RNAP-driven expression of 3WJ repressor index 20 switch RNA with a GFP-ASV reporter

    Depositor

    Alexander Green, Peng Yin

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_N20_switch
    

    Expression

    Bacterial

    Available Since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pYZ_3WJrep_N20_trigger

    Plasmid

    #132726

    Purpose

    T7 RNAP-driven expression of 3WJ repressor index 20 trigger RNA

    Depositor

    Alexander Green, Peng Yin

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_N20_trigger
    

    Expression

    Bacterial

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAVS1_Puro_PGK1_3xFLAG_Twin_Strep_EZH2

    Plasmid

    #79902

    Purpose

    Gene Addition of 3xFLAG_Twin_Strep_EZH2 to the AAVS1 Genomic Safe Harbor Locus

    Depositor

    Yannick Doyon

    Article

    Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

    Insert

    3xFLAG_Twin_Strep_EZH2
    

    Use

    CRISPR and TALEN

    Tags

    3xFLAG_Twin_Strep

    Expression

    Mammalian

    Promoter

    PGK1

    Available Since

    July 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYZ_3WJrep_N24_switch

    Plasmid

    #132725

    Purpose

    T7 RNAP-driven expression of 3WJ repressor index 24 switch RNA with a GFP-ASV reporter

    Depositor

    Alexander Green, Peng Yin

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    3WJ_N24_switch
    

    Expression

    Bacterial

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. Bxb1Donor_WT_ADRB2_LucReporter

    Plasmid

    #129788

    Purpose

    encodes a donor vector for recombination with the Bxb1 recombinase with the WT Beta-2 Adrenergic Receptor and a cAMP-responsive luciferase reporter gene

    Depositor

    Sriram Kosuri

    Article

    Jones et al Elife. 2020 Oct 21;9. pii: 54895. doi: 1

    Insert

    ADRB2 (ADRB2 Human)
    

    Use

    Synthetic Biology

    Tags

    3x FLAG

    Expression

    Mammalian

    Promoter

    N/A

    Available Since

    Sept. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPReporter

    Plasmid

    #65007

    Purpose

    E. coli reporter vector with MCS for inserting bacterial promoter region through 5' end of gene of interest, upstream and in-frame with reporter cloning site

    Depositor

    Mattheos Koffas

    Article

    Cress et al ACS Synth Biol. 2015 Mar 30.

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    none

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK_ToeRepG2_N02_switch

    Plasmid

    #132742

    Purpose

    T7 RNAP-driven expression of second-generation toehold repressor index 2 switch RNA with a GFP-ASV reporter

    Depositor

    Alexander Green, Peng Yin

    Article

    Kim et al Nat Chem Biol. 2019 Dec;15(12):1173-1182

    Insert

    ToeRepG2_N02_switch
    

    Expression

    Bacterial

    Available Since

    Nov. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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