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Showing: 41 - 60 of 476 results

  2. p426_Cas9_gRNA-ARS1206a

    Plasmid
    #87398
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1206a sequence CGAACATTTTTCCATGCGCT in yeast chromosome 12.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1206a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87398-plasmid-map-sequence-id-193441

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  3. p426_Cas9_gRNA-ARS1414a

    Plasmid
    #87400
    Purpose
    All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS1414a sequence GCGCCACAGTTTCAAGGGTC in yeast chromosome 14.
    Depositor
    Aindrila Mukhopadhyay
    Article
    Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-
    Insert
    Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS1414a
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    ADH1, pTyrosine
    Available since
    April 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    87400-plasmid-map-sequence-id-193443

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  7. R777-E109 Hs.IRS1

    Plasmid
    #70393
    Purpose
    Gateway ORF clone of human IRS1 [NM_005544.2] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    IRS1 (IRS1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70393-plasmid-map-sequence-id-125842

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  8. pAcBac1-Ma-Acridone-RS1 (A7)

    Plasmid
    #197566
    Purpose
    Expression of Methanomethylophilus alvus (Ma) Acridone RS1 (A7) tRNA synthetase/Pyl-tRNA pair for the encoding of Acridone at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    M. alvus PylRS - Acd RS1 (A7)
    M. alvus Pyl-tRNA (6) (4x copies)
    Use
    Tags
    None
    Expression
    Mammalian
    Mutation
    N166A, V168G, W239C
    Promoter
    CMV and U6/H1
    Available since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    197566-plasmid-map-sequence-id-388228

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  12. Fast-GCaMP-RS15

    Plasmid
    #52652
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Article
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-RS15
    Use
    Tags
    Expression
    Mammalian
    Mutation
    deleted G47/H48 from GCaMP3
    Promoter
    T7
    Available since
    May 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  13. Fast-GCaMP-RS14

    Plasmid
    #52651
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Article
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-RS14
    Use
    Tags
    Expression
    Mammalian
    Mutation
    deleted G47 from GCaMP3
    Promoter
    T7
    Available since
    May 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  14. Fast-GCaMP-RS12

    Plasmid
    #52650
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Article
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-RS12
    Use
    Tags
    Expression
    Mammalian
    Mutation
    deleted R40/R41/K42 from GCaMP3
    Promoter
    T7
    Available since
    May 6, 2014
    Availability
    Academic Institutions and Nonprofits only

  17. SARS1_NSP13_BV_NBIO-TEV_CTHROM-his

    Plasmid
    #234382
    Purpose
    Baculovirus expression for structure determination. May not contain entire coding region of gene.
    Depositor
    Cheryl Arrowsmith
    Insert
    Nsp13_SARS
    Use
    Baculovirus expresssion
    Tags
    LVPRGSGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSG
    Expression
    Mutation
    Promoter
    Polyhedrin
    Available since
    April 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    234382-plasmid-map-sequence-id-469458

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  19. SpyTag-Quartet [SARS1]

    Plasmid
    #214729
    Purpose
    In mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV each separated by GS-linkers with an N-terminal SpyTag fusion
    Depositor
    Mark Howarth
    Article
    Hills et al Nat Nanotechnol. 2024 May 6. doi: 10.103
    Insert
    SpyTag-Quartet [SARS1] (S Synthetic)
    Use
    Tags
    SpyTag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    214729-plasmid-map-sequence-id-435534

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Showing: 41 - 60 of 476 results