We narrowed to 35,108 results for: Sis;

1. pFS_0350_pET30-RT-CRISPR_Cellulomonas_bogoriensis_69B4_ARRAY_1_RC

   Plasmid

   #116962

   Purpose

   Expresses CbRT-Cas1-Cas2 under pT7lac promoter, encodes CbCRISPR array 1 RC, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Cellulomonas bogoriensis 69B4 RT-Cas1-Cas2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pmCherry_C1 I3(resist)

   Plasmid

   #61399

   Purpose

   Expresses mCherry tagged Inhibitor-3 resistant to I3 S2 siRNA in mammalian cells

   Depositor

   Hemmo Meyer

   Article

   Eiteneuer et al EMBO J. 2014 Oct 8. pii: e201489054.

   Insert

   Inhibitor-3 (PPP1R11 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   silent mutations of bases C183T T192C T189C

   Promoter

   CMV

   Available since

   Sept. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pR26 CAG AsiSI/MluI

   Plasmid

   #74286

   Purpose

   Gene targeting vector for the mouse Rosa26 locus, including a CAG promoter and loxP flanked stop cassette, for cloning into AsiSI or Mlui

   Depositor

   Ralf Kuehn

   Article

   Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

   Insert

   Rosa26 5-homology region
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-h53BP1 (siRNA resistant)

   Plasmid

   #110301

   Purpose

   Mammalian expression of a EGFP-tagged full length human 53BP1 (resistant to siRNA targeting AGAACGAGGAGACGGTAATAGTGGG)

   Depositor

   Chris Kok-Lung Chan

   Article

   Tiwari et al Nat Commun. 2018 Feb 14;9(1):677. doi: 1

   Insert

   p53-binding protein 1 (TP53BP1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   GAACGAGGA to GAGCGGGGC

   Promoter

   Available since

   May 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. BAP1-C91A (siRNA resistant)

   Plasmid

   #108438

   Purpose

   Stable expression of BAP1-C91A (catalytically inactive mutant) in mammalian cells, gene has an introduced siRNA-resistance against siBAP1.

   Depositor

   Emanuela Felley-Bosco, Rolf Stahel

   Article

   Okonska et al Mol Cancer Ther. 2019 Oct 16. pii: 1535-

   Insert

   BAP1 (BAP1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C91A: c.271T>G, c.272G>C; others: c.1641C&g…

   Promoter

   CMV

   Available since

   Nov. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLYS1-EMRE/RNAi resistant

   Plasmid

   #50056

   Purpose

   Expression of human EMRE in mammalian cells with silent mutation making it resistant to RNAi

   Depositor

   Vamsi Mootha

   Article

   Sancak et al Science. 2013 Nov 14.

   Insert

   EMRE-RNAi resistant (SMDT1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   RNAi resistant, silent mutant

   Promoter

   Available since

   Feb. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR FL CPAP RNAi resistant

   Plasmid

   #46390

   Depositor

   Pierre Gonczy

   Article

   Kitagawa et al J Cell Sci. 2011 Nov 15;124(Pt 22):3884-

   Insert

   CPAP RNAi resistant mutant (CENPJ Human)
   

   Use

   Entry vector

   Tags

   Expression

   Mutation

   silent mutation that renders it resistant to siRN…

   Promoter

   Available since

   July 12, 2013

   Availability

   Academic Institutions and Nonprofits only

8. 6975 pPROEX-HTb-SIS1

   Plasmid

   #1233

   Depositor

   Susan Lindquist

   Article

   Shorter et al Science 2004 Jun 18;304(5678):1793-7. Ep

   Insert

   sis1 (SIS1 Budding Yeast)
   

   Use

   Tags

   his tag (tev cleavable)

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 18, 2005

   Availability

   Academic Institutions and Nonprofits only

9. XE100 Pertussis toxin A protomer

   Plasmid

   #16678

   Depositor

   Randall Moon

   Article

   Slusarski et al Nature. 1997 Nov 27;390(6658):410-3. doi

   Insert

   Pertussis toxin A protomer (ptxA Bordetella pertussis)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

10. pBABE-Src-Dasatinib-resistant

    Plasmid

    #26980

    Depositor

    Joan Massague

    Article

    Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

    Insert

    c-Src (SRC Chicken)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    T338I

    Promoter

    Available since

    Jan. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFRT/TO/GFP-SCAF4 (CRISPR_resistant)

    Plasmid

    #122471

    Purpose

    Dox-inducible GFP (N-terminal tag) SCAF4 expression

    Depositor

    Jesper Svejstrup

    Article

    Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

    Insert

    SCAF4 (SCAF4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Synonymous mutations conferring resistance to gRN…

    Promoter

    CMV

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCDNA3_shRNA resistant PKA-Cα-mEGFP

    Plasmid

    #114143

    Purpose

    Mammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.

    Depositor

    Haining Zhong

    Article

    Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

    Insert

    mouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. E17TAG GFP zeo resistance

    Plasmid

    #69118

    Purpose

    For E17TAG GFP reporter expression in non-zeocin resistant strains

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    E17TAG GFP
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    E17 to TAG

    Promoter

    Available since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pEB1deltaC-2xEGFP shRNA resistant

    Plasmid

    #37925

    Depositor

    Torsten Wittmann

    Article

    Gierke et al Curr Biol. 2012 May 8;22(9):753-62. Epub

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deletion after aa248, TTCCTGT=>TCCCGGT

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFRT/TO/GFP-SCAF8 (CRISPR_resistant)

    Plasmid

    #122472

    Purpose

    Dox-inducible GFP (N-terminal tag) SCAF8 expression

    Depositor

    Jesper Svejstrup

    Article

    Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

    Insert

    SCAF8 (SCAF8 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Synonymous mutations conferring resistance to gRN…

    Promoter

    CMV

    Available since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRFP shRNA-resistant TrkB WT

    Plasmid

    #72714

    Purpose

    Mammalian expression of TrkB fused to RFP in C-terminus, shRNA resistant

    Depositor

    Freddy Jeanneteau

    Article

    Arango-Lievano et al Proc Natl Acad Sci U S A. 2015 Nov 16. p

    Insert

    TrkB
    

    Use

    Tags

    RFP

    Expression

    Mammalian

    Mutation

    shRNA-resistant TrkB (T300C, T301A, C302G, C306A,…

    Promoter

    CMV

    Available since

    Feb. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pET15b-Fab1 (Francisella tularensis)

    Plasmid

    #61690

    Purpose

    Inducible expression of the FabI enzyme (enoyl reductase) from Francisella tularensis

    Depositor

    Michael Johnson

    Article

    Hevener et al J Med Chem. 2012 Jan 12;55(1):268-79. do

    Insert

    enoyl-[acyl-carrier-protein] reductase (NADH)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

18. FLAG-PPP2R5E siRNA-resistant

    Plasmid

    #120861

    Purpose

    expresses dox-inducible PPP2R5E resistant to siRNA pool 2 used in manuscript

    Depositor

    Emily Foley

    Article

    Lee et al J Cell Sci. 2017 May 15;130(10):1760-177

    Insert

    PPP2R5E (PPP2R5E Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    silent mutation at aa 282-284 in PPP2RE to genera…

    Promoter

    Available since

    Feb. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX389 Fluviicola taffensis Cas9

    Plasmid

    #68326

    Purpose

    Fluviicola taffensis Cas9

    Depositor

    Feng Zhang

    Insert

    FtCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX378 Sutterella wadsworthensis Cas9

    Plasmid

    #68315

    Purpose

    Sutterella wadsworthensis Cas9

    Depositor

    Feng Zhang

    Insert

    SwCas9
    

    Use

    CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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