Addgene

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pET-22b(+)-HpENR

Plasmid

#12651

Depositor

SeWon Suh

Article

Lee et al Acta Crystallogr D Biol Crystallogr. 200

Insert

noyl-acyl carrier protein reductase (fabI Helicobacter pylori)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pET21a.MJ0285

Plasmid

#11304

Depositor

Sung-Hou Kim

Article

Kim et al J Struct Biol. 1998 Jan . 121(1):76-80.

Insert

small heat shock protein (MJ_0285 M. jannaschii)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 10, 2006

Availability

Academic Institutions and Nonprofits only

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pET28a-hdNadV

Plasmid

#83362

Purpose

Expresses nadV from Haemophilus ducreyi in Escherichia coli

Depositor

George Cătălin Marinescu

Article

Marinescu et al Sci Rep. 2018 Mar 13;8(1):4433. doi: 10.

Insert

nadV

Use

Tags

none

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 18, 2016

Availability

Academic Institutions and Nonprofits only

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TrmD_HaeIn/pET28b(+) N,C-tag

Plasmid

#12665

Depositor

SeWon Suh

Article

Kim et al Acta Crystallogr D Biol Crystallogr. 200

Insert

tRNA (guanine-N(1)-)-methyltransferase (trmD Haemophilus influenzae)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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RCAS Cyp26 (CC#62)

Plasmid

#15154

Depositor

Connie Cepko

Article

Sen et al Development. 2005 Dec . 132(23):5147-59.

Insert

Cyp26 (CYP26A1 Chicken)

Use

Retroviral; Avian expression

Tags

Expression

Mutation

Promoter

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pISH-Cck

Plasmid

#105989

Purpose

To prepare riboprobe for in situ hybridization

Depositor

Peter Mombaerts

Article

Walz et al J Neurobiol. 2006 Jul . 66(8):835-46.

Insert

cholecystokinin (Cck Mouse)

Use

To generate riboprobe

Tags

Expression

Mutation

Promoter

Available since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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LDB221

Plasmid

#185427

Purpose

Bacterial expression of Nup1 C-terminal residues 777-1076 as GST fusion

Depositor

Ken Belanger, Laura Davis

Article

Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

Insert

NUP1

Use

Tags

GST

Expression

Bacterial

Mutation

Nup1 C-terminal residues 777-1076

Promoter

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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LDB203

Plasmid

#185424

Purpose

Bacterial expression of N-terminus of NUP1 nucleoporin as GST-Nup1-N fusion

Depositor

Ken Belanger, Laura Davis

Article

Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

Insert

NUP1

Use

Tags

GST

Expression

Bacterial

Mutation

Nup1 N-terminal domain only

Promoter

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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LDB205

Plasmid

#185425

Purpose

Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion

Depositor

Ken Belanger, Laura Davis

Article

Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

Insert

NUP1

Use

Tags

GST

Expression

Bacterial

Mutation

Nup1 C-terminal domain only

Promoter

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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KBB101

Plasmid

#185064

Purpose

Bacterial expression of C-terminus of NUP1 nucleoporin as GST-Nup1-C fusion truncated after the last FXFG repeat

Depositor

Ken Belanger

Article

Belanger et al CBE Life Sci Educ. 2009 Fall;8(3):214-25

Insert

NUP1

Use

Tags

GST

Expression

Bacterial

Mutation

Nup1 C-terminal region, truncated

Promoter

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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HI0058/pET-21a(+)c-tag

Plasmid

#13097

Depositor

SeWon Suh

Article

Ku et al Acta Crystallogr D Biol Crystallogr. 200

Insert

3-deoxy-manno-octulosonate cytidylyltransferase (kdsB Haemophilus influenzae)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

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BS.YxiN.Twin1.a207-368.wt

Plasmid

#13531

Depositor

David McKay

Article

Caruthers et al Acta Crystallograph Sect F Struct Biol C

Insert

B. subtilis DEAD-box helicase YxiN second domain

Use

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

deleted amino acid residues 1-206 and 369-479

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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pBMNb-cALFA

Plasmid

#159991

Purpose

For the protein expression with ALFA tag at C-terminus in mammalian cells

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Type

Empty backbone

Use

Tags

HRV3C protease cleavage site-ALFA tag-twin Strep …

Expression

Mammalian

Mutation

CMV promoter, WPRE

Promoter

Available since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pRFHUE-eGFP

Plasmid

#89469

Purpose

GFP labelling of filamentous fungi by Agrobacterium tumefaciens mediated transformation

Depositor

Luis González-Candelas

Article

Crespo-Sempere et al Int J Food Microbiol. 2011 Aug 2;148(2):

Insert

eGFP

Use

Binary plasmid for atmt of filamentous fungi

Tags

Expression

Mutation

Promoter

A. nidulans gpdA

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pDawn-sfGFP

Plasmid

#107741

Purpose

Expresses superfolder GFP under the control of a blue-light transcriptional regulator

Depositor

Ingmar Riedel-Kruse

Article

Jin et al Proc Natl Acad Sci U S A. 2018 Apr 3;115

Insert

superfolder GFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

pLambda

Available since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS-ChrimsonR-mRuby2-ST

Plasmid

#105447

Purpose

Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

Insert

ChrimsonR-mRuby2-ST

Use

Tags

mRuby2 and soma targeting motif

Expression

Mammalian

Mutation

K176R in Chrimson

Promoter

pCAGGS

Available since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pGGF_RT_FAST_RFP

Plasmid

#136979

Purpose

Goldengate/Greengate module marker for transgenic Arabidopsis seed coat fluorescence

Depositor

Klaus Theres

Article

Rossmann et al Plant J. 2019 Oct 30. doi: 10.1111/tpj.1

Insert

seed coat marker RFP

Use

Tags

Expression

Bacterial

Mutation

BsaI site domestication in pOLE1:OLE1-RFP 688 G &…

Promoter

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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BbZIP-cALFA

Plasmid

#160498

Purpose

For the protein expression of BbZIP with ALFA tag at C-terminus in E. coli

Depositor

Motoyuki Hattori

Article

Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

Insert

BbZIP

Use

Tags

HRV3C protease cleavage site-ALFA tag-8histidine

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pRG/III-hs-MBP-T43NTR-TrxA-H10

Plasmid

#121677

Purpose

Bacterial expression of neurotensin receptor fusion protein

Depositor

Richard Henderson, Reinhard Grisshammer

Article

Grisshammer et al Protein Expr Purif. 1997 Oct;11(1):53-60

Insert

neurotensin receptor (Ntsr1 Rat)

Use

Tags

TrxA-H10

Expression

Bacterial

Mutation

deleted amino acids 1-42

Promoter

lac

Available since

March 5, 2019

Availability

Academic Institutions and Nonprofits only
C413-E22: attB4-polyhedrinp>-attB1r

Plasmid

#162922

Purpose

Gateway attB4/attB1r entry clone for baculovirus expression using the AcMNPV polyhedrin promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

AcMNPV polyhedrin promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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