We narrowed to 24,835 results for: ina
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Plasmid#58694PurposeExpresses truncated C-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with N-Terminal Split Cas9 Gyra Intein for full length SpCas9 productionDepositorInsertHumanized C-Terminal S. pyogenes Cas9 with GyrA Csplit Intein
UseAAV and CRISPRTagsGyrA Csplit Intein and NLSExpressionMammalianPromoterCBhAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-Rab35 S22N inactive
Plasmid#47426DepositorAvailable SinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
N-Terminal Split Cas9 with GyrA intein
Plasmid#58693PurposeExpresses truncated N-terminal SpCas9 domain fused to a GyrA intein, flanked by ITRs for AAV packaging. Combine with C-Terminal Split Cas9 Gyra Intein for full length SpCas9 productionDepositorInsertHumanized N-Terminal S. pyogenes Cas9 with GyrA Nsplit Intein
UseAAV and CRISPRTags3xFlag, GyrA Nsplit Intein, and NLSExpressionMammalianPromoterCBhAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
p53 (dominant negative R175H mutant)-pcw107-V5
Plasmid#64638Purpose3rd generation lentiviral transfer plasmid. When used to produce lentivirus, express physiological levels of insertDepositorAvailable SinceDec. 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLVX-EF1a-GFP-LaminA-IRES-Hygromycin
Plasmid#134867PurposeExpresses EGFP-tagged nuclear envelope markerDepositorAvailable SinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti
Plasmid#135955PurposeNeuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3bDepositorInsertPrime editor v2 (PE2) N-terminal
UseLentiviralExpressionMammalianPromoterhSynapsinAvailable SinceJan. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Myc-tagged delta N (dominant-negative form) human TCF4E pcDNA3
Plasmid#32739DepositorInsertTCF4E (Tcf7l2 Human)
TagsMycExpressionMammalianMutationThe N-terminal deletion form (delta N) lacks amin…Available SinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SpyTag002-MBP
Plasmid#102831PurposeBacterial expression of SpyTag002 fused to maltose binding proteinDepositorInsertSpyTag002-MBP
TagsHis6 and Thrombin cleavage siteExpressionBacterialMutationPeptide tag added to the N-terminus of MBP via a …PromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-SpyCatcher002
Plasmid#102829PurposeBacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagentsDepositorInsertCys-SpyCatcher002
TagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationCysteine residue in N-terminal regionPromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Avitag-SpyCatcher002
Plasmid#102828PurposeBacterial expression of SpyCatcher002 with a peptide tag enabling site-specific biotinylationDepositorInsertAvitag-SpyCatcher002
TagsAvitag, GSSGS, His6, and TEV cleavage siteExpressionBacterialPromoterT7Available SinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
lentiCas9-Blast
Plasmid#52962PurposeExpresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.DepositorHas ServiceLentiviral PrepInsertsCas9
Blasticidin resistance
UseCRISPR and LentiviralTagsFLAGExpressionMammalianPromoterEFS-NSAvailable SinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
lentiCas9-Blast (Lentiviral Prep)
Viral Prep#52962-LVPurposeReady-to-use Lentiviral Prep particles produced from lentiCas9-Blast (#52962). In addition to the viral particles, you will also receive purified lentiCas9-Blast plasmid DNA. Lentiviral particles carrying Cas9 and blasticidin resistance. This virus is used to make stable cell lines expressing Cas9.DepositorPromoterEFS-NSAvailable SinceJuly 14, 2016AvailabilityAcademic Institutions and Nonprofits only -
Human ubiquitination-related proteins CRISPR KO library
Pooled Library#174592PurposeTargets human ubiquitation-related proteins and deubiquitinating enzymes.DepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable SinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-pgk-Cre (AAV Retrograde)
Viral Prep#24593-AAVrgPurposeReady-to-use AAV Retrograde particles produced from AAV-pgk-Cre (#24593). In addition to the viral particles, you will also receive purified AAV-pgk-Cre plasmid DNA. Cre expression under the PGK promoter. These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons.DepositorPromoterpgkAvailable SinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Human Kinase Domain-Focused CRISPR Knockout Library
Pooled Library#117725PurposeDesigned for genomic deletion screening of protein kinases by targeting the kinase domains.DepositorExpressionMammalianSpeciesHomo sapiensUseCRISPR and LentiviralAvailable SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
hCRISPRa-v2, Kinases, Phosphatases, and Drug Targets (h1), top 5 sgRNAs/gene
Pooled Library#83980PurposeHuman CRISPRa Pooled Library tartgeting kinsases, phosphatases and Drug TargetsDepositorAvailable SinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCL-Eco
Plasmid#12371DepositorInsertgag/pol/env
UseRetroviralExpressionMammalianAvailable SinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K63
Plasmid#17606PurposeMammalian expression of HA tagged ubiquitin with K63 only, other lysines mutated to argininesDepositorInsertUbiquitin C (UBC Human)
TagsHAExpressionMammalianMutationK63 only, other lysines mutated to arginines. Enh…Available SinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-Ubiquitin-K48
Plasmid#17605PurposeMammalian expression of HA tagged ubiquitin with only K48, other lysines mutated to argininesDepositorInsertUbiquitin C (UBC Human)
TagsHAExpressionMammalianMutationK48 only, other lysines mutated to arginines. Enh…Available SinceMarch 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1
Plasmid#41393Purpose3rd generation, Inducible lentiviral expression, TRE-gateway; PGK-rtTA-2A-puroDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Gateway destination vector, doxycycli…ExpressionMammalianPromoterTRE promoter, Tet ONAvailable SinceNov. 30, 2012AvailabilityAcademic Institutions and Nonprofits only