Addgene

We narrowed to 11,728 results for: cel.2;

Showing: 581 - 600 of 11728 results

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P-del (pTransluc-miR-145-2)

Plasmid

#21500

Depositor

Kenneth Kosik

Article

Xu et al Cell. 2009 May 15. 137(4):647-58.

Insert

0.9 kb 5' of miR-145 (MIR145 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

June 23, 2009

Availability

Academic Institutions and Nonprofits only

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pJ204-loxP-100RPU.2-ETN-lox2272

Plasmid

#154833

Purpose

Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of etanercept

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Insert

ETN

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

100RPU.2

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pJ204-loxP-100RPU.2-coEPO-lox2272

Plasmid

#154828

Purpose

Donor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing one copy of erythropoietin

Depositor

Lise Marie Grav

Article

Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102

Insert

coEPO

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Codon-optimized (Cricetulus griseus)

Promoter

100RPU.2

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO human shRNA 2 c-MAF

Plasmid

#29433

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 13. ():.

Insert

c-MAF shRNA 2 (MAF Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2011

Availability

Academic Institutions and Nonprofits only
pMKO.1 puro p53 shRNA 2

Plasmid

#10672

Depositor

William Hahn

Article

Masutomi et al Cell. 2003 Jul 25. 114(2):241-53.

Insert

p53 shRNA (TP53 Human)

Use

RNAi and Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2006

Availability

Academic Institutions and Nonprofits only

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pPGK-T7/2-CD44v6-10 (fl)

Plasmid

#137820

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v6-10 (fl) (CD44 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

full length

Promoter

PGK

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pPGK-T7/2-CD44v8-10 (fl)

Plasmid

#137823

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v8-10 (fl) (CD44 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

full length

Promoter

PGK

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pBiFC-HA-hCaspase 2 prodomain-VC155

Plasmid

#49261

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 prodomain (CASP2 Human)

Use

Tags

HA and split Venus C

Expression

Mammalian

Mutation

delete aa 148-435

Promoter

CMV

Available since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pBIFC-flag-hCaspase 2 prodomain-VN173

Plasmid

#49262

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 prodomain (CASP2 Human)

Use

Tags

flag and split Venus N

Expression

Mammalian

Mutation

delete aa 148-435

Promoter

CMV

Available since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pBiFC-HA-hCaspase 2 CARD-VC155

Plasmid

#49259

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

Caspase-2 CARD (CASP2 Human)

Use

Tags

HA and split Venus C

Expression

Mammalian

Mutation

deleted aa 123-435

Promoter

CMV

Available since

Dec. 9, 2013

Availability

Academic Institutions and Nonprofits only
pBiFC-flag-hCaspase 2 CARD-VN173

Plasmid

#49260

Purpose

Expresses split fluorescence protein to measure caspase-2 dimerization in mammalian cells.

Depositor

Douglas Green

Article

Bouchier-Hayes et al Mol Cell. 2009 Sep 24;35(6):830-40. doi:

Insert

caspase-2 CARD (CASP2 Human)

Use

Tags

Flag and split Venus N

Expression

Mammalian

Mutation

delete aa 123-435

Promoter

CMV

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only
pPGK-T7/2-CD44v6-10 (-cyt)

Plasmid

#137821

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v6-10 (-cyt) (CD44 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region

Promoter

PGK

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pPGK-T7/2-CD44v8-10 (-cyt)

Plasmid

#137822

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v8-10 (-cyt) (CD44 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region

Promoter

PGK

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pPGK-T7/2-CD44v2-10 (fl)

Plasmid

#137824

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v2-10 (fl) (CD44 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

full length and mutations A282T, T483A, N535D, S5…

Promoter

PGK

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pPGK-T7/2-CD44v2-10 (-cyt)

Plasmid

#137825

Purpose

expression of CD44 proteins in mammalian cells

Depositor

Ursula Gunthert

Article

Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

Insert

CD44v2-10 (-cyt) (CD44 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

without cytoplasmic region - N213S on the CD44 re…

Promoter

PGK

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pαH-S-GSAS-OMICRON.BA.2-RBD

Plasmid

#184830

Purpose

Mammalian cell expression of RBD of SARS-CoV-2 spike protein of variant OMICRON.BA.2 with furin side replaced by GSAS and with 2 Proline substitution at 986 and 987

Depositor

Priyamvada Acharya

Article

Stalls et al Cell Rep. 2022 Jun 28;39(13):111009. doi

Insert

S-GSAS-OMICRON.BA2-RBD (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

6X His tag

Expression

Mammalian

Mutation

SARS-CoV-2 Spike RBD protein of OMICRON.BA.2 vari…

Promoter

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-S (Wuhan)

Plasmid

#215812

Purpose

Expresses SARS-CoV-2 Spike protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Spike (S )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-HiBiT-N

Plasmid

#215815

Purpose

Expresses N protein with HiBiT tag in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 HiBiT Nucleocapsid (N )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SARS-CoV-2-S (D614G)

Plasmid

#215838

Purpose

Expresses SARS-CoV-2 Spike protein in mammalian cells

Depositor

Thomas Gallagher

Article

Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

Insert

SARS-CoV-2 Spike (S )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-GFP-G3BP1(2)

Plasmid

#136059

Purpose

G3BP1 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GTATTACACACTGCTGAACC)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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