Addgene

We narrowed to 2,334 results for: crispr system

Showing: 581 - 600 of 2334 results

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p426*-SpSgH

Plasmid

#136993

Purpose

Helper plasmid for SpCas9 gRNA cloning and expression

Depositor

Huimin Zhao

Article

Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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p426*-LbSgH

Plasmid

#136992

Purpose

Helper plasmid for LbCpf1 gRNA cloning and expression

Depositor

Huimin Zhao

Article

Lian et al Nat Commun. 2019 Dec 19;10(1):5794. doi:

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

May 21, 2020

Availability

Academic Institutions and Nonprofits only

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DmU6 3p3 GFP LgRNA

Plasmid

#182207

Purpose

For generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Drosophila

Depositor

Craig Montell

Article

Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

Insert

3XP3-GFP

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pJMP2480

Plasmid

#160080

Purpose

Z. mobilis CRISPRi, promoter C, empty guide

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2132

Plasmid

#160075

Purpose

Z. mobilis CRISPRi, promoter B, empty guide

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pJMP2046

Plasmid

#160073

Purpose

Z. mobilis CRISPRi, promoter A, empty guide, sfGFP

Depositor

Jason Peters

Article

Banta et al Appl Environ Microbiol. 2020 Nov 10;86(2

Insert

empty sgRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pU6-gRNA

Plasmid

#53062

Purpose

Transcription of guide RNA (gRNA) in wheat cells

Depositor

Caixia Gao

Article

Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

Insert

wheat U6 promoter - gRNA

Use

CRISPR; Plant expression

Tags

Expression

Mutation

Promoter

wheat U6

Available since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only

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pYPQ131B2.0

Plasmid

#99885

Purpose

Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtU3

Available since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ132B2.0

Plasmid

#99888

Purpose

Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtU3

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pYPQ133B2.0

Plasmid

#99892

Purpose

Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtU3

Available since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462)

Plasmid

#48141

Purpose

NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
pRS156

Plasmid

#182707

Purpose

CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

Depositor

Rebecca Shapiro

Article

Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BE-zCas9

Plasmid

#101739

Purpose

expression of an optimized Cas9 for base-editing in zebrafish

Depositor

Shuo Lin

Article

Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

Insert

rAPOBEC1-XTEN-zCas9-UGI-NLS

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A

Promoter

CMV

Available since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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A3Bctd-max

Plasmid

#198888

Purpose

Express A3Bctd-max BE4max-based base editor

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)

Use

Tags

6x His and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pACYC-T7-Nla-repeat-BbsI

Plasmid

#180215

Purpose

For cloning Neisseria lactamica Type I-C crRNA for protein purification in E.Coli

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Type

Empty backbone

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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HiFi dCas9 VPR

Plasmid

#188510

Purpose

Expresses FLAG tagged HiFi dCas9 VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A/R691A/H840A

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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AsdCas12a VPR

Plasmid

#188512

Purpose

Expresses FLAG tagged AsdCas12a VPR

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12a

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D908A

Promoter

EF1a

Available since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-BE-VQRCas9

Plasmid

#101740

Purpose

expression of an optimized Cas9 for base-editing in zebrafish

Depositor

Shuo Lin

Article

Zhang et al Nat Commun. 2017 Jul 25;8(1):118. doi: 1

Insert

rAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS

Use

CRISPR

Tags

Expression

Mammalian

Mutation

D10A, VQR mutations

Promoter

CMV

Available since

Jan. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRH2502

Plasmid

#84379

Purpose

Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter

Depositor

Robert Husson

Article

Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

Insert

dcas9

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Aspartate at position 10 changed to Alanine, Hist…

Promoter

uv15tetO

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pMK-Cas9-gate

Plasmid

#113743

Purpose

Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants.

Depositor

Magdalena Bezanilla

Article

Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

Insert

Cas9

Use

CRISPR; Gateway destination vector

Tags

Expression

Mutation

Promoter

Z. Mays ubiquitin promoter, 35S promoter

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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