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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,171 results for: tef

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Showing: 581 - 600 of 3171 results

  5. BB3cK_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104910
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418
    Depositor
    Brigitte Gasser
    Publication
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    April 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    104910-plasmid-map-sequence-id-206061

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  9. pAAH1

    Plasmid
    #41825
    Purpose
    Used as a PCR template for tagging yeast proteins with the DHFR tag (c-terminal histag) by homologous recombination and selection for hygromycin resistance.
    Depositor
    Melissa Moore
    Publication
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Expression
    Bacterial and Yeast
    Promoter
    T7, TEF
    Available since
    Oct. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    41825-plasmid-map-sequence-id-56469

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  10. pAAH6

    Plasmid
    #41830
    Purpose
    Used as a PCR template for tagging yeast proteins with the SNAP tag by homologous recombination and selection for ClonNAT resistance
    Depositor
    Melissa Moore
    Publication
    Hoskins et al Science. 2011 Mar 11;331(6022):1289-95.
    Type
    Empty backbone
    Use
    Pcr template
    Expression
    Bacterial and Yeast
    Promoter
    T7,TEF
    Available since
    Oct. 29, 2013
    Availability
    Academic Institutions and Nonprofits only
    41830-plasmid-map-sequence-id-69212

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  13. pCASB

    Plasmid
    #190175
    Purpose
    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence
    Depositor
    Jessica Tyler
    Publication
    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:
    Insert
    sgRNA cloning site
    Expression
    Bacterial and Yeast
    Mutation
    Added 5'-agagacc-3' upstream and 5'…
    Available since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    190175-plasmid-map-sequence-id-372326

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  15. pBV65

    Plasmid
    #108363
    Purpose
    A nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.
    Depositor
    Scott Moye-Rowley
    Publication
    Vu et al mSphere. 2018 Mar 28;3(2). pii: mSphere0
    Insert
    loxP-natMX6-CgMET3prom-cre-loxP
    Use
    Gene disruption marker for candida glabrata
    Promoter
    C. glabrata MET3
    Available since
    May 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    108363-plasmid-map-sequence-id-209915

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  19. pMZ376

    Plasmid
    #74213
    Purpose
    Combined sgRNA/Cas9 vector with KanMX marker
    Depositor
    Mikel Zaratiegui
    Inserts
    Cas9
    rrk1:sgRNA
    Use
    CRISPR
    Expression
    Yeast
    Mutation
    Silent muation of the CspCI site
    Promoter
    adh1 and rrk1
    Available since
    March 31, 2016
    Availability
    Academic Institutions and Nonprofits only
    74213-plasmid-map-sequence-id-139302

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Showing: 581 - 600 of 3171 results