We narrowed to 14,429 results for: nts

1. pRS416-Aga1p-FAPB2.3.6L1TAG

   Plasmid

   #158148

   Purpose

   Yeast display reporter for non-display yeast strains (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   Aga1p-FAPB2.3.6
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Cloned in a TAG codon at the first position of th…

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL2-100bp

   Plasmid

   #16294

   Depositor

   Wafik El-Deiry

   Article

   Wang et al Cancer Res. 2006 Jul 15. 66(14):6982-9.

   Insert

   p53 promoter fragment (TP53 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   fragment -88 to +12 relative to first transcripti…

   Promoter

   Available since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJG363

   Plasmid

   #91183

   Purpose

   BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase (nAtCas9_D10A+gNt_R2+donor)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   nAtCas9_D10A+gNt_R2+donor
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJG370

   Plasmid

   #91187

   Purpose

   BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A single nickase no gRNA control (nAtCas9_D10A+gRNActrl+donor)

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   nAtCas9_D10A+gRNActrl+donor
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL2-50bp

   Plasmid

   #16295

   Depositor

   Wafik El-Deiry

   Article

   Wang et al Cancer Res. 2006 Jul 15. 66(14):6982-9.

   Insert

   p53 promoter fragment (TP53 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   fragment -38 to +12 relative to first transcripti…

   Promoter

   Available since

   Dec. 14, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGWB528

   Plasmid

   #74870

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with TAP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   July 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMOD_A0601

   Plasmid

   #91016

   Purpose

   Module A, Promoter: 35S, Gene: Csy4-P2A-AtCas9_D10A nickase, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Csy4-P2A-AtCas9_D10A nickase
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A

   Promoter

   35S

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCTCON2-GFP-TAG

   Plasmid

   #158131

   Purpose

   Single fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)

   Depositor

   James Van Deventer

   Article

   Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

   Insert

   sfGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Introduced a stop codon in place of the tyrosine …

   Promoter

   GAL1-10

   Available since

   Jan. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pDGE652

   Plasmid

   #153230

   Purpose

   Recipient plant transformation vector containing selection markers and a Cas9 expression cassette, but lacking guide RNAs

   Depositor

   Sylvestre Marillonnet

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FusionRed-VASP-5

    Plasmid

    #56136

    Purpose

    Localization: Focal Adhesions, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    VASP
    

    Use

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    A209T in VASP

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pcDNA3 WT MYOC eGLuc2 FLAG

    Plasmid

    #192820

    Purpose

    Expresses wild-type myocilin with a C-terminal enhanced Gaussia luciferase and FLAG tag

    Depositor

    John Hulleman

    Article

    Nakahara et al Curr Eye Res. 2022 Jun;47(6):918-922. do

    Insert

    myocilin (MYOC Human)
    

    Use

    Tags

    FLAG and enhanced Gaussia luciferase (M43I/M110I)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBABE-neo largeTcDNA delta434-444

    Plasmid

    #8582

    Depositor

    Bob Weinberg

    Article

    Hahn et al Mol Cell Biol 2002 Apr;22(7):2111-23.

    Insert

    large T antigen (SV40gp6 simian virus)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    deletion in part of the bipartite p53 binding dom…

    Promoter

    Available since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

13. mCer-Vamp2(qv/fw)

    Plasmid

    #53238

    Purpose

    Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    VAMP2 (Vamp2 Rat)
    

    Use

    Tags

    4aa-linker and mCerulean

    Expression

    Mammalian

    Mutation

    Mutated Q76 to V; F77 to W.

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

14. pAAV-TRE-DIO-eYFP-f

    Plasmid

    #104057

    Purpose

    An AAV genome with tet-inducible, Cre-dependent expression of the fluorescent protein eYFP with a C-terminal Hras farnesylation sequence

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Insert

    EYFP-f
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUSE-Src-KD-UniRapR-mCerulean-myc

    Plasmid

    #45382

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Proc Natl Acad Sci U S A. 2013 Apr 23;11

    Insert

    Src-KD-UniRapR-mCerulean-myc
    

    Use

    Tags

    mCerulean and myc

    Expression

    Mammalian

    Mutation

    mSrc R388D (kinase dead), Y529F

    Promoter

    CMV

    Available since

    Aug. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3 IKKe T7

    Plasmid

    #27238

    Depositor

    Tom Maniatis

    Article

    Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

    Insert

    I-kappa-B kinase epsilon (IKBKE Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pMOD_C2813

    Plasmid

    #91092

    Purpose

    Module C, Promoter: PvUbi2, Gene: Csy4 (+ BaeI site for GT donor cloning), Terminator: pea rbcsE9

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    Csy4 (+ BaeI site for GT donor cloning)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    PvUbi2

    Available since

    July 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA_DiCre_59.F2

    Plasmid

    #60207

    Purpose

    Expresses a fusion protein of FKBP12-linker peptide 2- Cre(19-59)

    Depositor

    Jean-Paul Herman

    Article

    Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

    Insert

    Cre recombinase (CD207 Bacteriophage P1, Synthetic, Human)
    

    Use

    Tags

    FKBP12 and Nuclear localisation signal

    Expression

    Mammalian

    Mutation

    amino acids 19-59 of the humanized version of Cre…

    Promoter

    CMV

    Available since

    Jan. 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHA-ΔN335-Kin1A

    Plasmid

    #31606

    Depositor

    Anthony Brown

    Article

    Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

    Insert

    ΔN335 kinesin-1A (Kif5a Mouse)
    

    Use

    Tags

    HA-tag

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-335

    Promoter

    CMV

    Available since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

20. KHBD00233

    Plasmid

    #34377

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG7803 (z Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only