Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 25,285 results for: crispr

Clear keywords
Showing: 6001 - 6020 of 25285 results

  1. pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)

    Plasmid
    #154840
    Purpose
    Insertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.
    Depositor
    Patrick Phillips
    Publication
    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.
    Insert
    homology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
    Use
    CRISPR and Cre/Lox
    Expression
    Worm
    Mutation
    HYGR∆ encodes aa 1-226
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    154840-plasmid-map-sequence-id-307910

    Enlarge

  4. pUC_sgRNA

    Plasmid
    #68710
    Purpose
    Contains the sgRNA backbone sequence (tracrRNA, 82 bp) and is used as DNA template to amplify a specific sgRNA using a forward primer with the protospacer sequence for gene targeting.
    Depositor
    Roberto Docampo
    Publication
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Available since
    Sept. 14, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    5 Articles
    68710-plasmid-map-sequence-id-123992

    Enlarge

  5. px330_SMAD exon 9 gRNA

    Plasmid
    #68352
    Purpose
    px330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter.
    Depositor
    Jannik Elverløv-Jakobsen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SMAD exon 9 gRNA
    Expression
    Mammalian
    Promoter
    U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68352-plasmid-map-sequence-id-119624

    Enlarge

  6. pY111 (pcDNA3.1-huTsCpf1)

    Plasmid
    #92267
    Purpose
    Expression of humanized TsCpf1
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    huTsCpf1
    Tags
    NLS and NLS-3xHA
    Expression
    Mammalian
    Available since
    July 18, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    92267-plasmid-map-sequence-id-183046

    Enlarge

  18. pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

    Plasmid
    #112922
    Purpose
    Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker
    Depositor
    Kosuke Yusa
    Publication
    Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo
    Use
    Gateway Cloning
    Expression
    Mammalian
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    112922-plasmid-map-sequence-id-217731

    Enlarge

  19. pT2K-CAGGS-U6-sgRNA-M5-U6-sgRNA-M7-U6-sgRNA-M9-IRES-CFP

    Plasmid
    #114729
    Purpose
    3 sgRNAs targeting a sequence upstream of the initiator ATG of the cellular Myc gene
    Depositor
    Martine Roussel
    Publication
    Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA M5, sgRNA M7, sgRNA M9
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Sept. 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    114729-plasmid-map-sequence-id-222610

    Enlarge

  20. pMOD8A-iRGR-r11

    Plasmid
    #74369
    Purpose
    This plasmid constitutively expresses gRNA-r11 from an AHD1 promoter using the insulated RGR design. Integrates in the W303 HIS locus and restores HIS function.
    Depositor
    Eric Klavins
    Publication
    Gander et al bioRxiv 041871
    Insert
    RGR-r11
    Use
    CRISPR and Synthetic Biology
    Expression
    Yeast
    Promoter
    AHD1
    Available since
    April 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    74369-plasmid-map-sequence-id-141324

    Enlarge

Showing: 6001 - 6020 of 25285 results