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Str-STIM1-NN_ST-SBP-mCherry

Plasmid

#65263

Purpose

synchronize trafficking of ST from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Str-STIM1-NN and truncated sialyltransferase (ST6GAL1 Human)

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Mutation

contains only amino acids 1 to 43 of ST

Promoter

CMV

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only

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pMslc

Plasmid

#32095

Depositor

Christopher Buck

Article

Schowalter et al PLoS Pathog. 2011 Jul;7(7):e1002161. Epu

Insert

SLC35A1 (SLC35A1 Human)

Expression

Mammalian

Promoter

FerH

Available Since

Nov. 4, 2011

Availability

Academic Institutions and Nonprofits only

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G protein alpha-s-CFP

Plasmid

#55793

Purpose

G protein alpha-s internally tagged with ECFP and EE epitope

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

G-alpha-s-EE-ECFP (Gnas Rat)

Tags

ECFP flanked by SGGGS on each side was inserted i…

Expression

Mammalian

Mutation

His was substituted for Asn164 in ECFP (Clontech)…

Promoter

CMV

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pJAT17

Plasmid

#204298

Purpose

For introducing Cas9 under D. melanogaster nanos promoter. Markers with EYFP expressed in eyes.

Depositor

David Stern

Article

Stern et al bioRxiv 2023.06.17.545412

Insert

3XP3-EYFP

Use

CRISPR

Promoter

3XP3

Available Since

Aug. 31, 2023

Availability

Academic Institutions and Nonprofits only

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CDKN1A sgRNA

Plasmid

#138189

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDKN1A

Depositor

Wen Xue

Article

Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

Insert

CDKN1A (CDKN1A Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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G-GECO1-Orai1

Plasmid

#73561

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2016 Jan 12;11

Insert

NM_032790.3 (ORAI1 Human, Synthetic)

Tags

G-GECO1

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

March 10, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-G6-vioABE

Plasmid

#73438

Purpose

Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.

Depositor

Mattheos Koffas

Article

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

PG6-vioABE

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

PG6 (orthogonal T7-lac variant)

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pIS1 IMP-1 long UTR

Plasmid

#21640

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

IGF-II mRNA-binding protein 1 UTR (IGF2BP1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-mouse Oct4

Plasmid

#21110

Depositor

Dean Tantin

Article

Kang et al Genes Dev. 2009 Jan 15. 23(2):208-22.

Insert

Oct4 (Pou5f1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only
MSCV FLIPi P2G_Thy1.1 Dbl (p53,PTEN)

Plasmid

#19746

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting p53 and PTEN (Pten Mouse)

Use

Cre/Lox, RNAi, and Retroviral

Expression

Mammalian

Available Since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA-p160C

Plasmid

#46

Depositor

Bruce Spiegelman

Article

Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

Insert

p160 myb binding protein (Mybbp1a Mouse)

Tags

myc

Expression

Mammalian

Mutation

expresses aa580-1330

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA4/TO/GFP-TTP

Plasmid

#141190

Purpose

Expresses GFP-TTP in mammalian cells, can be used to make inducible cell line

Depositor

Simon Bekker-Jensen

Article

Tollenaere et al Cell Rep. 2019 Mar 26;26(13):3511-3521.e

Insert

TTP (ZFP36 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA4c/His-Xpress-HA-hBIG1(E793K)

Plasmid

#79433

Purpose

Expresses N-terminally His6, Xpress and HA-tagged BIG1(E793K) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Shin et al Mol Biol Cell. 2004 Dec;15(12):5283-94.

Insert

BIG1 (ARFGEF1 Human)

Tags

HA, His6, and Xpress

Expression

Mammalian

Mutation

changed Glutamic acid 793 to Lysine

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only
FRP1641_PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Plasmid

#58441

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 3 lexA boxes

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Expression

Yeast

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Available Since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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pGL3Basic-miR126-EGFL7-Promoter

Plasmid

#32244

Depositor

Charles Lowenstein

Article

Harris et al Arterioscler Thromb Vasc Biol. 2010 Oct;

Insert

miR-126 Promoter 5' UTR (MIR126, EGFL7 Human)

Use

Luciferase

Expression

Mammalian

Mutation

Promoter region 5' UTR

Promoter

5' UTR

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pPV-H1-ccdB-mEF1α-RiH

Plasmid

#100598

Purpose

piggyBac vector for sgRNA cloning

Depositor

Akitsu Hotta

Article

Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

Type

Empty backbone

Use

Piggybac vector

Expression

Mammalian

Promoter

Human H1 PolIII promoter, human FTH1 promoter, mo…

Available Since

April 2, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ALK6 Q203D

Plasmid

#80883

Purpose

mammalian expression of ALK6 Q203D

Depositor

Aristidis Moustakas

Article

Raja et al Oncotarget. 2016 Jan 12;7(2):1120-43. do

Insert

ALK6 (Bmpr1b Mouse)

Tags

HA

Expression

Mammalian

Mutation

Q203D (constitutively active)

Promoter

CMV

Available Since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only
pBabe JunD-HA neo

Plasmid

#58486

Purpose

mammalian expression of mouse JunD

Depositor

Kevin Janes

Article

Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do

Insert

JunD (Jund Mouse)

Tags

HA

Expression

Mammalian

Available Since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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rEc2

Bacterial Strain

#51946

Purpose

oriT-kan template

Depositor

Farren Isaacs

Article

Ma et al Nat Protoc. 2014 Oct;9(10):2285-300. doi

Bacterial Resistance

Kanamycin

Available Since

May 1, 2014

Availability

Academic Institutions and Nonprofits only
pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE (AAV1)

Viral Prep

#202199-AAV1

Purpose

Ready-to-use AAV1 particles produced from pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE (#202199). In addition to the viral particles, you will also receive purified pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE plasmid DNA. Synapsin-driven, Cre-dependent expression of bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized opsin PdCO. These AAV preparations are suitable purity for injection into animals.

Depositor

Ofer Yizhar

Article

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Promoter

Syn

Tags

EGFP

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only