We narrowed to 32,650 results for: Eng

1. pcDNA3.3-hALDOA D34S

   Plasmid

   #210810

   Purpose

   To reduce the catalytic constant (kcat) for conversion of FBP to dihydroxyacetone phosphate (DHAP) and glyceraldehyde-3-phosphate (G3P)

   Depositor

   Sheng-cai Lin

   Article

   Zhang et al Nature. 2017 Aug 3;548(7665):112-116. do

   Insert

   ALDOA (ALDOA Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   residues 34 of ALDOA mutated to serine

   Promoter

   CMV

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. BE3-hA3A-Y130F

   Plasmid

   #132945

   Purpose

   Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

   Depositor

   Hui Yang

   Article

   Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

   Insert

   BE3-hA3A(Y130F)
   

   Use

   CRISPR; Base editor

   Expression

   Mammalian

   Mutation

   BE3-hA3A(Y130F)

   Available Since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV V5-TbID-emerin

   Plasmid

   #175099

   Purpose

   Lentiviral expression of V5-TbID-tagged mouse Emd

   Depositor

   Larry Gerace

   Article

   Cheng et al J Proteome Res. 2022 Sep 2;21(9):2197-22

   Insert

   Emerin (Emd Mouse)
   

   Use

   Lentiviral

   Tags

   V5-TbID

   Expression

   Mammalian

   Available Since

   Jan. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMT-zic2a-cytoBirA-2A-mCherry_Ras

   Plasmid

   #80064

   Purpose

   Zic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Promoter

   Zic2a enhancer driving c-fos promoter

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMT-ubb-NLS-BirA-2a-mCherry

   Plasmid

   #79886

   Purpose

   Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Tags

   3x HA

   Expression

   Bacterial

   Promoter

   Ubiquitin

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMT-MCS-NLS-BirA-2A-mCherry_Ras

   Plasmid

   #80059

   Purpose

   MCS for cloning promoter to drive HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
   

   Use

   Zebrafish biotagging

   Tags

   3x HA

   Expression

   Bacterial

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. Cas9_YTHDF2_sgRNA

   Plasmid

   #186673

   Purpose

   YTHDF2 sgRNA plasmid

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   YTHDF2 KO sgRNA Plasmid (YTHDF2 Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-GABARα1N-HA

   Plasmid

   #128110

   Purpose

   Express GABARα1 N terminal domain

   Depositor

   Huda Zoghbi

   Article

   Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

   Insert

   Gabra1 (Gabra1 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEM-BirA-2A-H2B-Dendra2-SV40pA-FKF

   Plasmid

   #119864

   Purpose

   Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), H2B-tagged Dendra2 fluorophore and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

   Depositor

   Tatjana Sauka-Spengler

   Article

   Weinberger et al Dev Cell. 2020 Mar 9;52(5):574-590.e6. d

   Insert

   BirA, 2A, H2B-tagged Dendra2 and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
   

   Tags

   HA-tagged biotin ligase (BirA), Thosea asigna vir…

   Expression

   Bacterial

   Available Since

   March 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Mouse GFP-PRMT7 LDIG(69-72)AAAA

    Plasmid

    #65044

    Purpose

    Expresses Catalytically inactive GFP-PRMT7 LDIG(69-72)AAAA mutant

    Depositor

    Steven Clarke

    Article

    Feng et al J Biol Chem. 2013 Dec 27;288(52):37010-2

    Insert

    PRMT7 (Prmt7 Mouse)
    

    Use

    Bacterial propagation

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    L69A D70A I71A G72A

    Promoter

    CMV

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

11. tdEos-Keratin-17

    Plasmid

    #57629

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

    Insert

    Keratin (KRT18 Human)
    

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available Since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. Egr1-1POK(E239Y,L349K)-dMBP-V5

    Plasmid

    #239428

    Purpose

    Egr1 signal monomer

    Depositor

    Changyang Linghu

    Article

    Zheng et al bioRxiv 2025.05.10.653182

    Insert

    CytoTape-V5 (EGR1 Synthetic)
    

    Use

    AAV

    Tags

    V5-dMBP

    Expression

    Mammalian

    Promoter

    Egr1 promoter

    Available Since

    Oct. 29, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. PATagRFP-GRP78

    Plasmid

    #238419

    Purpose

    Expresses GRP78 fused to the photoactivatable Tag-red fluorescent protein (PATagRFP-GRP78)

    Depositor

    Amy Shiu Lee

    Article

    Tseng et al Life Sci Alliance. 2019 Aug 15;2(4):e201

    Insert

    78-kDa glucose-regulated protein (HSPA5 Human)
    

    Tags

    PATagRFP

    Expression

    Mammalian

    Available Since

    Sept. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. GST-GRP78-N Terminus (pGEX4T1)

    Plasmid

    #238422

    Purpose

    Expresses GRP78 N terminus fused to the GST Tag

    Depositor

    Amy Shiu Lee

    Article

    Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

    Insert

    78-kDa glucose-regulated protein (HSPA5 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. GST-GRP78-C Terminus (pGEX4T1)

    Plasmid

    #238423

    Purpose

    Expresses GRP78 C Terminus fused to the GST Tag

    Depositor

    Amy Shiu Lee

    Article

    Tseng et al Neoplasia. 2019 Aug;21(8):837-848. doi:

    Insert

    78-kDa glucose-regulated protein (HSPA5 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Aug. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX4T-1 AMPK αKD(27-290aa)

    Plasmid

    #227853

    Purpose

    To express in E. coli AMPK αKD(27-290aa)

    Depositor

    Sheng-cai Lin

    Article

    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

    Insert

    AMPK (PRKAA1 Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 27-290 only

    Available Since

    July 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA 3.3 N-MYC TB182

    Plasmid

    #227882

    Purpose

    Used for transfection of cells for expression of C-MYC TB182

    Depositor

    Sheng-cai Lin

    Article

    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

    Insert

    TB182 (TNKS1BP1 Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Available Since

    July 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA 3.3 N-MYC PDHA1

    Plasmid

    #227877

    Purpose

    Used for transfection of cells for expression of C-MYC PDHA1

    Depositor

    Sheng-cai Lin

    Article

    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

    Insert

    PDHA1 (PDHA1 Human)
    

    Tags

    MYC

    Expression

    Mammalian

    Available Since

    July 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA 3.3 C-MYC pdzd-8(536A)

    Plasmid

    #227885

    Purpose

    Used for transfection of cells for expression of C-MYC pdzd-8(536A)

    Depositor

    Sheng-cai Lin

    Article

    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

    Insert

    pdzd-8 (C53B4.4 Nematode)
    

    Tags

    MYC

    Expression

    Mammalian

    Mutation

    S536A

    Available Since

    July 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA 3.3 N-MYC pdzd-8

    Plasmid

    #227844

    Purpose

    Used for transfection of cells for expression of C-MYC pdzd-8

    Depositor

    Sheng-cai Lin

    Article

    Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

    Insert

    pdzd-8 (C53B4.4 Nematode)
    

    Tags

    MYC

    Expression

    Mammalian

    Available Since

    June 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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