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TagBFP2- PIP4K2BD278K

Plasmid

#202729

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:PIP4K2B (D278K) (PIP4K2B Human)

Expression

Mammalian

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z31

Plasmid

#120074

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with V5

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

V5

Use

Pcr template

Tags

V5

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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TOPO GFP

Plasmid

#68488

Purpose

TOPO construct expressing GFP for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Green Fluorescent Protein

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pETSG

Plasmid

#159418

Purpose

Expression of proteins with a C-terminal thermostable GFP (TGP) tag in Escherichia coli

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

thermostable GFP (TGP)

Tags

polyhistidine (poly-His)

Expression

Bacterial

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Blast-moxGFP

Plasmid

#207677

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Blast-2A-moxGFP cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pFUS_B4

Plasmid

#31021

Depositor

Adam Bogdanove, Daniel Voytas

Article

Cermak et al Nucleic Acids Res. 2011 Jul;39(12):e82.

Insert

LacZ + BsaI restriction sites

Available Since

June 30, 2011

Availability

Academic Institutions and Nonprofits only

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Human p150,95

Plasmid

#8633

Depositor

Timothy Springer

Article

Corbi et al EMBO J 1987 Dec 20;6(13):4023-8.

Insert

Human p150,95 (ITGAX Human)

Expression

Mammalian

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-LIC-V5

Plasmid

#120248

Purpose

For LIC cloning with C-terminal V5 tag

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

V5

Promoter

EF1a

Available Since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Zeo-3xFLAG

Plasmid

#207706

Purpose

Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-3xFLAG cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Phat2-dr-cPla2-C2-EGFP-6H

Plasmid

#187001

Purpose

Protein Expression

Depositor

Philipp Niethammer

Article

Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

Insert

ENSDARG00000024546 (pla2g4aa Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

Aug. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pRS426 Gal-RNQ1-YFP

Plasmid

#18686

Depositor

Susan Lindquist

Article

Douglas et al Proc Natl Acad Sci U S A. 2008 May 20. 1

Insert

Rnq1 (RNQ1 Budding Yeast)

Tags

YFP

Expression

Yeast

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only
p3XFLAG-CMV 7.1_syn6

Plasmid

#50012

Purpose

Encodes syntaxin 6 with a N-terminal 3X FLAG tag

Depositor

David Hackstadt

Article

Moore et al Microbiology. 2011 Mar;157(Pt 3):830-8.

Insert

syntaxin 6 (Stx6 Rat)

Tags

3x FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pRosa26 R1-ccdB-R2 RexNeo PI-SceI

Plasmid

#24418

Depositor

Edward Hsiao

Article

Hsiao et al Stem Cell Res Ther. 2011 Mar 4. 2(2):11.

Insert

Use

Mouse Targeting

Available Since

March 16, 2011

Availability

Academic Institutions and Nonprofits only

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Flag-Rap1b

Plasmid

#118325

Purpose

Expression of bovine Rap1b

Depositor

Philip Stork

Article

Takahashi et al J Biol Chem. 2013 Sep 27;288(39):27712-2

Insert

Rap1b (RAP1B Bovine)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pMXs-Dppa3

Plasmid

#13365

Depositor

Shinya Yamanaka

Article

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

developmental pluripotency associated 3 (Dppa3 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available Since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pOGG037

Plasmid

#113995

Purpose

SC module. Super-folder GFP green fluorescence reporter

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Super-folder GFP

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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Cdk9-Halo donor

Plasmid

#113113

Purpose

Donor plasmid for knock-in HaloTag fusing to the c-terminal of mouse Cdk9 coding sequence

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

Cdk9 donor region including homology arms and HaloTag

Use

Synthetic Biology

Available Since

Aug. 6, 2018

Availability

Academic Institutions and Nonprofits only

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FepA

Plasmid

#26039

Depositor

Johann Deisenhofer

Article

Buchanan et al Nat Struct Biol. 1999 Jan . 6(1):56-63.

Insert

FepA (fepA E Coli)

Expression

Bacterial

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only
pMB1609_pRR-EGFP

Plasmid

#65852

Purpose

EGFP-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9

Depositor

Marc Bühler

Article

Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:

Insert

split EGFP coding sequence

Use

CRISPR and TALEN

Expression

Mammalian

Promoter

SV40

Available Since

Aug. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pCMU-AUTr

Plasmid

#61204

Purpose

Fluorescent reporter for autophagosomes mCherry-MtLC3 expressed under AtUBQ10 promoter

Depositor

Maria Harrison

Article

Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

Insert

LC3(ATG8)

Tags

mCherry

Expression

Plant

Promoter

AtUBQ10

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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